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Cao Li‑chuang, Liu Yu-huan, Xie Wei.*, and Wang Z.(*)Structures of a glucose-tolerant β-glucosidase provide insights into its mechanism. Journal of Structural Biology. 2017,198 (3): 154-162.
Xiaolong Liu, Mingjun Liang 1 Yuhuan Liu and Xinjiong Fan*. Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability. Microb Cell Fact. 2017. 16:81
Guang-hui Ren, Li-chuang Cao, Wei Kong, Zhi-jun Wang, and Yu-huan Liu* . Efficient Secretion of the β‑Galactosidase Bgal1‑3 via both Tat- Dependent and Tat-Independent Pathways in Bacillus subtilis. J. Agric. Food Chem. 2016, 64, 5708−5716
Xiwen Liu, Li-chuang Cao, Xin-jiong Fan, Yu-huan Liu* & Wei Xie*. Engineering of a thermostable esterase Est816 to improve its quorum- quenching activity and the underlying structural basis. Scientific Reports. 2016, 6:38137
Lichuang Cao, Zhijun Wang, Guang‑hui Ren, Wei Kong, Liang Li, Wei Xie and Yuhuan Liu*. Engineering a novel glucose-tolerant β- glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration. Biotechnol. Biofuels. 2015, 8:202. 2015.
Qingnan Tian, Caiyan Wang, Yuhuan Liu and Wei Xi*. Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase. Nucleic Acids Research. 2015, 43: 5, 2015
Li-chuang Cao#, Ran Chen#, Wei Xie*, and Yuhuan Liu*. Enhancing the Thermostability of Feruloyl Esterase EstF27 by Directed Evolution and the Underlying Structural Basis. J. Agric. Food Chem. 2015, 63, 8225−8233.
Liang Li#, Gang Li#, Li-chuang Cao, Guang-hui Ren, Wei Kong, Si-di Wang, Geng-shan Guo,and YuHuan Liu*. Characterization of the Cross-Linked Enzyme Aggregates of a Novel β-Galactosidase, a Potential Catalyst for the Synthesis of Galacto-Oligosaccharides. J. Agric. Food Chem. 2015, 63, 894−901.
Jian Yao, Geng Shan Guo, Guang Hui Ren, Yuhuan Liu*. Production, characterization and applications of tannase. J. Mol. Catal. B: Enzym. 2014, 101: 137– 147.
Si-di Wang, Geng-shan Guo, Liang Li, Li-chuang Cao, Ling Tong,Guang-hui Ren, Yuhuan Liu*. Identification and characterization of an unusualglycosyltransferase-like enzyme with β-galactosidaseactivity from a soil metagenomic library. Enzyme and Microbial Technology, 2014, 57: 26–35.
Jian Yao, Qinglong Chen, Guoxiang Zhong, Wen Cao, An Yu and Yuhuan Liu*.Immobilization and Characterization of Tannase from a Metagenomic Library and Its Use for Removal of Tannins from Green Tea Infusion J. Microbiol. Biotechnol. 2014, 24(1): 80–86.
Jian Yao, Qing Long Chen, Ai Xi Shen, Wen Cao, Yuhuan Liu*. A novel feruloyl esterase from a soil metagenomic library with tannase activity. J. Mol. Catal. B: Enzym. 2013, 95: 55– 61.
Sidi Wang, Kui Wang, Liang Li , Yuhuan Liu*. Isolation and characterization of a novel organic solvent-tolerant and halotolerant esterase from a soil metagenomic library. J. Mol. Catal. B: Enzym. 2013, 95: 1– 8.
Xia Zhang#, He Li#, Chang-Jie Li, Teng Ma, Gang Li *.and Yuhuan Liu*. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of TurpanBasin. BMC Microbiol. 2013, 13:237.
Gang Li, Yang Jiang, Xin-jiong Fan, Yuhuan Liu*.Molecular cloning and characterization of a novel b-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil metagenomic library. Bioresource Technol. 2012, 123; 15–22.
Xinjiong Fan, Xiaolong Liu, Yuhuan Liu*. The cloning and characterization of one novel metagenome-derived thermostable esterase acting on N-acylhomoserine lactones. J. Mol. Catal. B: Enzym. 2012, 83:29-37.
Xinjiong Fan, Xiaolong Liu, Rui Huang and Yuhuan Liu*. Identification and characterization of a novel thermostable pyrethroid-hydrolyzing enzyme isolated through metagenomic approach. Microbial Cell Factories. 2012, 11:33.
Shi-Qin Yu, Yuhuan Liu*. Expression and one-step purification of β-galactosidase by fusion with elastin-like polypetides. Process Biochem. 2012, 47: 1108–1114,
Kui Wang, Yi Lu, Wei Qu Liang, Si Di Wang, Yang Jiang, Rui Huang, and Yu Huan Liu*. Enzymatic Synthesis of Galactooligosaccharides in an Organic−Aqueous Biphasic System by a Novel β-Galactosidase from a Metagenomic Library. J. Agric. Food Chem. 2012, 60, 3940−3946,
Shangke Chen, Kui Wang, Yuhuan Liu* and Xiaopeng Hu*. Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library. Acta Crystallographica Section F Structural Biology and Crystallization Communications (Acta Cryst. ). 2012, F68: 767-770.
Jian Yao, Xin Jiong Fan, Yi Lu, Yuhuan Liu*. Isolation and Characterization of a Novel Tannase from a Metagenomic Library. J. Agric. Food Chem. 2011, 59: 3812–3818.
Xinjiong Fan, Xiaolong Liu, Kui Wang, Sidi Wang, Rui Huang, Yuhuan Liu*. Highly soluble expression and molecular characterization of an organic solvent-stable and thermotolerant lipase originating from the met genome. J. Mol. Catal. B: Enzym. 2011, 72: 319– 326.
Shu Li Sang, Gang Li, Xiao Peng Hu, Yuhuan Liu*. Molecular Cloning, Overexpression and Characterization of a Novel Feruloyl Esterase from a Soil Metagenomic Library. J. Mol. Microbiol. Biotechnol. 2011, 20:196–203.
Liping Zhang, Kui Wang, ZhongxingMo, Yuhuan Liu* and Xiaopeng Hu*. Crystallization and preliminary X-ray analysis of a cold-active β- galactosidase from the psychrotrophic and halotolerant Planococcus sp. L4. Acta Crystallographica Section F Structural Biology and Crystallization Communications (Acta Cryst. ). 2011, F67: 911–913.
Yuhuan Liu, Mao Ye, Yi Lu, Xia Zhang and Gang Li. Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution. Appl. Microbiol .Biotechnol. 2011, 91:667–675.
Ye M, Li G, Liang WQ, Y.H Liu*. Molecular cloning and characterization of a novel metagenome-derived multicopper oxidase with alkaline laccase activity and highly soluble expression. Appl.Microbiol.Biotechnol. 2010, 87:1023-1031.
Kui Wang, Gang Li, Shi Qin Yu, Chen Ting Zhang, Yuhuan Liu*. A novel metagenome-derived β-galactosidase: gene cloning, overexpression, purification and characterization. Appl. Microbiol. Biotechnol. 2010, 88:155–165.
Gang Li, Kui Wang, Yuhuan Liu*. Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the Metagenome. Microbial Cell Factories. 2008, 7:38.
Lixiang Cao, Min Jiang, Zhirui Zeng, Aixue Du, Hongming Tan, Yuhuan Liu*. Trichoderma atroviride F6 improves phytoextraction efficiency of mustard (Brassica juncea (L.) Coss. var. foliosa Bailey) in Cd, Ni contaminated soils. Chemosphere. 2008, 71: 1769–1773
Ji M. Hu, He. Li, Li X. Cao, Pei C.Wu, Chen T. Zhang, Shu L.Sang, Xiao Y. Zhang, Min J.Chen, Jia Q. Lu, Yuhuan Liu*. Molecular cloning and characterization of the gene encoding cold-active β-galactosidase from a psychrotrophic and halotolerant Planococcus sp. L4. J. Agric. Food Chem. 2007, 55:2217-2224.
Li HUAPING, Lian JIE, Wang ZHIFENG, Zhong YINGCHANG, and Liu Yuhuan*. Cloning and Functional Expression of Ubiquitin-Like Protein Specific Proteases Genes from Candida. Biol. Pharm. Bull. 2007, 30(10): 1851—1855.
32. He Li, Yuhuan Liu* ,Na Luo, Xiao Y. Zhang, Tian G. Luan, Ji M. Hu, Zhuo Y. Wang, Pei C. Wu, Min J. Chen, Jia Q. Lu. Biodegradation of benzene and its derivatives by a psychrotolerant and moderately haloalkaliphilic Planococcus sp. strain ZD22. Research in Microbiology. 2006, 157:629-636.
33. H. Li, W.Q. Liang, Z.Y. Wang, N. Luo, X.Y. Wu, J.M. Hu, J.Q. Lu, X.Y. Zhang, P.C. Wu and Yuhuan Liu*,Enhanced production and partial characterization of thermostable α-galactosidase by thermotolerant Absidia sp.WL511. World Journal of Microbiology & Biotechnology, 2006, 22:1–7.
34. Pei C. Wu,Yuhuan Liu*, Zhuo Y. Wang, He Li, Wei Q. Liang, Na Luo, Ji M. Hu, Xiao Y. Zhang, Jia Q. Lu, Tian G. Luan and Li X. Cao. Molecular cloning, Purification and Biochemical Characterization of A Novel pyrethroid-hydrolyzing esterase From Klebsiella sp. strain ZD112. J. Agric. Food Chem. 2006, 54: 836-842.
35. W.Q. Liang, Z.Y. Wang, He Li, P. C. WU, J. M. HU, N. LUO, L. X. CAO, and Yuhuan Liu*. Purification and Characterization of a novel pyrethroid hydrolase from Aspergillus niger ZD11. J. Agric. Food Chem.. 2005, 53: 7415-7420.
36. Liu Yang, Zhou Shining, Chen Zhishi, Zhong Yingchang, Liu Yuhuan*. 2004. Purification and Characterization of an Isoflavone Conjugates-Hydrolyzing β-Glucosidase from Endophytic Bacterium. J. Agric. Food Chem. 2004, 52: 1940—1944.
37. Yuhuan Liu*, Liu yang, Chen zhi-shi, Lian jie, Huang xiao, and Chung ying-cheng. Purification and characterization of a novel organophosphorus pesticide hydrolase from Penicillium lilacinum BP303. Enzyme and Microbial Technology. 2004, 34:297-303.
38. Zhang Qing, Liu Yang, Yuhuan Liu*. Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168. FEMS Microbiology Letters, 2003, 228:39-44.
39. Yuhuan Liu*, Cheng, Y C., Xiong Y. Purification and characterization of Dimethoate-degrading enzyme of Aspergillus niger ZHY-256 isolated from sewage. 2001, Applied and Environmental Microbiology. 2001, 67: 3746-3749.
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