个人简介

1963 Ph.D., Cornell University 1960 B.S., Massachusetts Institute of Technology

研究领域

Analytical Chemistry, Bioanalysis, Proteomics

Prof. Karger’s research focuses on the development and application of microscale separations and MS analysis to problems of biological relevance. Current research involves (1) comprehensive characterization of complex proteins at the low fmole level using LC coupled to a hybrid linear ion trap – FT mass spectrometer; (2) ultra-narrow (5-20 µm i.d.) monolithic and porous layer open tubular columns for low attomole LC/MS proteomic analysis; (3) proteomic tissue analysis for disease biomarker discovery using 10,000 cells or less; and (4) multiplex LC coupled to MALDI-MS using a 2 kHz repetition rate laser. Professor Karger’s research group collaborates with clinicians, microbiologists and fundamental cell biologists to apply the developed technologies. As an example, the group is interacting with an industrial group on the discovery and validation of biomarkers for low and high grade stained cells from Pap smears (cervical scrapings). In another example, they are collaborating with a microbiologist on the study of growth of anaerobic bacteria (Archaea). The discovery of overexpressed enzymes has led to an understanding of important pathways for energy conservation. In a third example, the group is studying the kinetics of phosphorylation of tyrosine kinase receptors (EGFR) upon signal stimulation in order to elucidate important signaling pathways in tumor cancer cells. In these and other examples, the technology in ultratrace LC/MS allows generation of information not readily obtained by current methodologies.

近期论文

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An Integrated Platform for Isolation, Processing and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood S Li, BD Plouffe, AM Belov, S Ray, X Wang, SK Murthy, BL Karger, ... Molecular & Cellular Proteomics, mcp. M114. 045724 Immunological mechanisms of the antitumor effects of supplemental oxygenation SM Hatfield, J Kjaergaard, D Lukashev, TH Schreiber, B Belikoff, R Abbott, ... Science translational medicine 7 (277), 277ra30-277ra30 Physical detection of influenza A epitopes identifies a stealth subset on human lung epithelium evading natural CD8 immunity DB Keskin, BB Reinhold, GL Zhang, AR Ivanov, BL Karger, EL Reinherz Proceedings of the National Academy of Sciences 112 (7), 2151-2156 Constitutively oxidized CXXC motifs within the CD3 heterodimeric ectodomains of the T cell receptor complex enforce the conformation of juxtaposed segments. KN Brazin, RJ Mallis, C Li, DB Keskin, H Arthanari, Y Gao, SL Wu, ... Journal of Biological Chemistry 290 (1), 239-239 Pathway analysis and transcriptomics improve protein identification by shotgun proteomics from samples comprising small number of cells-a benchmarking study J Sun, GL Zhang, S Li, AR Ivanov, D Fenyo, F Lisacek, SK Murthy, ... BMC genomics 15 (Suppl 9), S1 Systemic oxygenation weakens the hypoxia and hypoxia inducible factor 1α-dependent and extracellular adenosine-mediated tumor protection SM Hatfield, J Kjaergaard, D Lukashev, B Belikoff, TH Schreiber, ... Journal of Molecular Medicine 92 (12), 1283-1292 Development of LC-MS methods for quantitation of hepcidin and demonstration of siRNA-mediated hepcidin suppression in serum S Li, T Nakayama, A Akinc, SL Wu, BL Karger Journal of pharmacological and toxicological methods Near‐zero growth kinetics of Pseudomonas putida deduced from proteomic analysis NS Panikov, M Mandalakis, S Dai, LR Mulcahy, W Fowle, WS Garrett, ... Environmental microbiology Constitutively oxidized CXXC motifs within the CD3 heterodimeric ectodomains of the T cell receptor complex enforce the conformation of juxtaposed segments KN Brazin, RJ Mallis, C Li, DB Keskin, H Arthanari, Y Gao, SL Wu, ... Journal of Biological Chemistry 289 (27), 18880-18892 Narrow ID monolithic capillary columns for high efficiency separation and high sensitivity analysis of biomolecules AR Ivanov, L Zang, BL Karger US Patent 8,691,088 480 Identification of novel small molecules as selective PAK4 allosteric modulators (PAMs) by stable isotope labeling of amino acids in cells (SILAC) W Senapedis, Y Landesman, M Schenone, B Karger, S Wu, S Shacham, ... European Journal of Cancer 50, 156 Tumor antigens as proteogenomic biomarkers in invasive ductal carcinomas LR Olsen, B Campos, O Winther, DC Sgroi, BL Karger, V Brusic BMC medical genomics 7 (Suppl 3), S2 Narrow bore porous layer open tube capillary column and uses thereof BL Karger, J Zhang US Patent 8,580,570 Comparative proteomic analysis reveals mechanistic insights into Pseudomonas putida F1 growth on benzoate and citrate M Mandalakis, N Panikov, S Dai, S Ray, BL Karger AMB Express 3 (1), 64 EQUILIBRIA IN HIGH-PERFORMANCE LIQUID BL Karger, JN LePage, N Tanakat High-Performance Liquid Chromatography: Advances and Perspectives, 113 Narrow bore porous layer open tube capillary column and uses thereof BL Karger, J Zhang US Patent App. 14/048,378 Congratulations, Frank Svec! BL Karger Journal of Separation Science 36 (17) Monoclonal antibody based biomarker discovery and development platform L Takacs, A Guttman, WS Hancock, BL Karger, M Duval, P Berna US Patent 8,512,959 Human folliculin delays cell cycle progression through late S and G2/M-phases: effect of phosphorylation and tumor associated mutations LA Laviolette, J Wilson, J Koller, C Neil, P Hulick, T Rejtar, B Karger, ... PloS one 8 (7), e66775 Comparability analysis of anti-CD20 commercial (rituximab) and RNAi-mediated fucosylated antibodies by two LC-MS approaches C Li, A Rossomando, SL Wu, BL Karger MAbs 5 (4), 565-575