Schilling, Thomas - University of California, Irvine - Department of Developmental and Cell Biology

研究领域

Zebrafish developmental genetics – Our laboratory uses genetics and molecular biology to study pattern formation in the early zebrafish embryo. The rapid development and simple anatomy of this teleost embryo, together with recently developed techniques for reverse genetics and a nearly complete genome sequence, make zebrafish a powerful molecular genetic system for studying the mechanisms of development. We focus on two areas: (i) neural crest specification and formation of the skeleton in the early embryo and (ii) long-range signals, morphogens, that pattern the anterior-posterior (A-P) axis of the nervous system. In both cases, we are interested in how gene functions translate into cell behaviours and the formation of tissues and organs. A major focus of the work in our laboratory is to define as fully as possible the genetic and molecular pathways that establish the neural crest and its derivatives. Neural crest is a population of highly migratory cells that arise from the crests of the forming neural tube as it folds, and give rise to all of the body’s pigmentation, most of its peripheral nervous system, as well as the head skeleton. We have been analyzing and cloning the genes underlying a large library of mutations identified by their defects in the craniofacial skeleton. We have also identified downstream targets that we now believe control neural crest migration, and these share many similarities with other migrating cell types, such as metastatic cancer cells. A second, more recent focus of our lab is to understand the genetic and molecular pathways that specify the identities of cells along the A-P axis in the early embryo, including neural crest. Here we have begun to take a more computational, systems approach. The vitamin A derivative, retinoic acid (RA), is thought to be a diffusible factor that promotes posterior development. Zebrafish mutants in an enzyme that synthesizes RA, called Retinaldehyde dehydrogenase (Raldh2), have defects in the formation of segments in the hindbrain, known as rhombomeres, that each contain unique sets of interneurons and motor neurons. More recently, my lab has studied requirements for: 1) other Raldh enzymes, 2) RA receptors (RARs), 3) cellular RA binding proteins (CRABPs), and 4) RA degrading enzymes (Cyp26s), in signaling.

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Wang W, Holmes WR, Sosnik J, Schilling TF and Nie Q (2017). Cell sorting and noise-induced plasticity coordinate to sharpen boundaries between gene expression domains. PLoS Computational Biology 13(1):e1005307. doi: 10.1371/journal.pcbi.1005307. Muto A and Schilling TF (2017). Zebrafish as a model to study cohesin and cohesinopathies. In K Yokomori and Katsuhiko Shirahige (Eds.). Cohesin and Condensin: Methods and Protocols. Methods in Mol Biol. Vol 1515, pp. 177-196 Springer Press. Vibert L, Aquino G, Gehring I, Subkhankulova T, Schilling TF, Rocco A and Kelsh RN (2016). An ongoing role for Wnt signaling in differentiating melanocytes in vivo. Pigment Cell Melanoma Research doi: 10.1111/pcmr.12568. Nichols J, Dowd J, Watson S, Parthasarathy R, Brooks E, Subramanian A, Nachtrab G, Poss KD, Schilling TF, and Kimmel CB. (2016). Repetitive element silencing buffers a mef2ca dependent ligament versus bone fate decision in the zebrafish craniofacial skeleton. Development 143, 4430-4440. Schilling TF, Sosnik J and Q Nie (2016). Visualizing retinoic acid morphogen gradients. In HW Detrich III, M Westerfield and LI Zon (Eds.). The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology (pp. 139-163). Methods in Cell Biol Vol 133. Elsevier Inc., Academic Press. Kawauchi S, Santos R, Muto A, Lopez-Burks M, Schilling TF, Lander AD and Calof AL (2016). Using vertebrate animal models to understand the etiology of developmental defects in Cornelia de Lange Syndrome. Am J Hum Genet C Semin Med Genet doi:10.1002/ajmg.c.31484. Sosnik J, Zheng L, Rackauckas CV, Gratton E, Nie Q, and Schilling TF. (2016). Noise modulation of retinoic acid signaling controls sharpening of segmental gene expression boundaries in the zebrafish hindbrain. eLife 5:e14034. doi:10.7554/eLife.14034. Le Pabic P, Cooper J and Schilling TF (2016). Developmental basis of phenotypic integration in two Lake Malawi cichlids. EvoDevo 21 7:3 doi: 10.1186/s13227-016-0040-z. Subramanian A, and Schilling TF (2015). Tendon development and musculoskeletal assembly: emerging roles for the extracellular matrix. Development 142, 4191-4204. doi: 10.1242/dev.114777. Cruz IA, Kappedal R, Mackenzie S, Hoffman TL, Schilling TF and Raible DW (2015). Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance. Developmental Biology 402, 229-238. doi: 10.1016/j.ydbio.2015.03.019. Boer, EF, Howell, ED, Schilling, TF, Jette, CA and Stewart RA (2015). Fascin1-dependent filopodia are required for directional migration of a subset of neural crest cells. PLoS Genetics 11(1):e1004946. doi: 10.1371/journal.pgen.1004946. Le Pabic P, Ng C and Schilling TF (2014). Fat-Dachsous signaling coordinates cartilage differentiation and polarity during craniofacial development. PLoS Genetics 10(10):e1004726. doi: 10.1371/journal.pgen.1004726. Muto A, Ikeda S, Lopez-Burks ME, Kikuchi Y, Calof AL, Lander AD and Schilling TF (2014). Nipbl and Mediator cooperatively regulate gene expression to control limb development. PLoS Genetics 10(9):e1004671. doi: 10.1371/journal.pgen.1004671. Alexander C, Piloto S, Le Pabic P and Schilling TF (2014). Wnt signaling interacts with Bmp and Edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics 10(7):e1004479. doi: 10.1371/journal.pgen.1004479. Subramanian A and Schilling TF (2014). Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions. eLife (Cambridge) doi: 10.7554/eLife.02372.