个人简介

Ph.D., Analytical Chemistry, University of Nebraska-Lincoln, 1999 B.S., Biochemistry, Union College, 1995 B.S., Molecular Biology, Union College, 1994

研究领域

Bioanalytical Chemistry and Mass Spectrometry

Current research projects in his laboratory include (1) investigations of kinase conformation to understand regulation and aberrant signaling in various disease states including cancer, (2) analysis of the conformation of viral accessory proteins from HIV, (3) studies of protein conformation at biological membranes, and (4) optimization and methods development in hydrogen exchange mass spectrometry.

近期论文

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Hydrogen/deuterium exchange mass spectrometry applied to IL-23 interaction characteristics: potential impact for therapeutics. Iacob RE, Krystek SR, Huang RY, Wei H, Tao L, Lin Z, Morin PE, Doyle ML, Tymiak AA, Engen JR, Chen G. Expert Rev Proteomics. 2015 Apr;12(2):159-69. doi: 10.1586/14789450.2015.1018897. Epub 2015 Feb 24. PMID: 25711416 Related citations Select item 25684709 Steric gate residues of Y-family DNA polymerases DinB and pol kappa are crucial for dNTP-induced conformational change. Nevin P, Engen JR, Beuning PJ. DNA Repair (Amst). 2015 Feb 4. pii: S1568-7864(15)00024-5. doi: 10.1016/j.dnarep.2015.01.012. [Epub ahead of print] PMID: 25684709 Related citations Select item 25684204 Inhibition of Pro-Apoptotic BAX by a Noncanonical Interaction Mechanism. Barclay LA, Wales TE, Garner TP, Wachter F, Lee S, Guerra RM, Stewart ML, Braun CR, Bird GH, Gavathiotis E, Engen JR, Walensky LD. Mol Cell. 2015 Mar 5;57(5):873-86. doi: 10.1016/j.molcel.2015.01.014. Epub 2015 Feb 12. PMID: 25684204 Related citations Select item 25398026 Applications of hydrogen/deuterium exchange MS from 2012 to 2014. Pirrone GF, Iacob RE, Engen JR. Anal Chem. 2015 Jan 6;87(1):99-118. doi: 10.1021/ac5040242. Epub 2014 Nov 14. No abstract available. PMID: 25398026 Related citations Select item 25223306 The influence of adnectin binding on the extracellular domain of epidermal growth factor receptor. Iacob RE, Chen G, Ahn J, Houel S, Wei H, Mo J, Tao L, Cohen D, Xie D, Lin Z, Morin PE, Doyle ML, Tymiak AA, Engen JR. J Am Soc Mass Spectrom. 2014 Dec;25(12):2093-102. doi: 10.1007/s13361-014-0973-1. Epub 2014 Sep 16. PMID: 25223306 Related citations Select item 25193673 A conserved isoleucine maintains the inactive state of Bruton's tyrosine kinase. Boyken SE, Chopra N, Xie Q, Joseph RE, Wales TE, Fulton DB, Engen JR, Jernigan RL, Andreotti AH. J Mol Biol. 2014 Oct 23;426(21):3656-69. doi: 10.1016/j.jmb.2014.08.018. Epub 2014 Sep 2. PMID: 25193673 Related citations Select item 25144189 Differential sensitivity of Src-family kinases to activation by SH3 domain displacement. Moroco JA, Craigo JK, Iacob RE, Wales TE, Engen JR, Smithgall TE. PLoS One. 2014 Aug 21;9(8):e105629. doi: 10.1371/journal.pone.0105629. eCollection 2014. PMID: 25144189 Free PMC Article Related citations Select item 24937475 Electron transfer control in soluble methane monooxygenase. Wang W, Iacob RE, Luoh RP, Engen JR, Lippard SJ. J Am Chem Soc. 2014 Jul 9;136(27):9754-62. doi: 10.1021/ja504688z. Epub 2014 Jun 24. PMID: 24937475 Related citations Select item 24813614 GroEL/ES chaperonin modulates the mechanism and accelerates the rate of TIM-barrel domain folding. Georgescauld F, Popova K, Gupta AJ, Bracher A, Engen JR, Hayer-Hartl M, Hartl FU. Cell. 2014 May 8;157(4):922-34. doi: 10.1016/j.cell.2014.03.038. PMID: 24813614 Related citations Select item 24613485 Conformational analysis of processivity clamps in solution demonstrates that tertiary structure does not correlate with protein dynamics. Fang J, Nevin P, Kairys V, Venclovas C, Engen JR, Beuning PJ. Structure. 2014 Apr 8;22(4):572-81. doi: 10.1016/j.str.2014.02.001. Epub 2014 Mar 6. PMID: 24613485 Free PMC Article Related citations Select item 24512177 A conformational investigation of propeptide binding to the integral membrane protein γ-glutamyl carboxylase using nanodisc hydrogen exchange mass spectrometry. Parker CH, Morgan CR, Rand KD, Engen JR, Jorgenson JW, Stafford DW. Biochemistry. 2014 Mar 11;53(9):1511-20. doi: 10.1021/bi401536m. Epub 2014 Feb 26. PMID: 24512177 Free PMC Article Related citations Select item 24259466 Therapeutic targeting of oncogenic K-Ras by a covalent catalytic site inhibitor. Lim SM, Westover KD, Ficarro SB, Harrison RA, Choi HG, Pacold ME, Carrasco M, Hunter J, Kim ND, Xie T, Sim T, Jänne PA, Meyerson M, Marto JA, Engen JR, Gray NS. Angew Chem Int Ed Engl. 2014 Jan 3;53(1):199-204. doi: 10.1002/anie.201307387. Epub 2013 Nov 20. PMID: 24259466 Free PMC Article Related citations Select item 24215480 Hydrogen exchange-mass spectrometry measures stapled peptide conformational dynamics and predicts pharmacokinetic properties. Shi XE, Wales TE, Elkin C, Kawahata N, Engen JR, Annis DA. Anal Chem. 2013 Dec 3;85(23):11185-8. doi: 10.1021/ac403173p. Epub 2013 Nov 14. PMID: 24215480 Free PMC Article Related citations Select item 24136070 Investigating monoclonal antibody aggregation using a combination of H/DX-MS and other biophysical measurements. Iacob RE, Bou-Assaf GM, Makowski L, Engen JR, Berkowitz SA, Houde D. J Pharm Sci. 2013 Dec;102(12):4315-29. doi: 10.1002/jps.23754. Epub 2013 Oct 17. PMID: 24136070 Free PMC Article Related citations Select item 24035710 Conformational transition of membrane-associated terminally acylated HIV-1 Nef. Akgun B, Satija S, Nanda H, Pirrone GF, Shi X, Engen JR, Kent MS. Structure. 2013 Oct 8;21(10):1822-33. doi: 10.1016/j.str.2013.08.008. Epub 2013 Sep 12. PMID: 24035710 Free PMC Article Related citations Select item 24018862 Analysis of overlapped and noisy hydrogen/deuterium exchange mass spectra. Guttman M, Weis DD, Engen JR, Lee KK. J Am Soc Mass Spectrom. 2013 Dec;24(12):1906-12. doi: 10.1007/s13361-013-0727-5. Epub 2013 Sep 10. PMID: 24018862 Free PMC Article Related citations Select item 23982207 Activation loop dynamics determine the different catalytic efficiencies of B cell- and T cell-specific tec kinases. Joseph RE, Kleino I, Wales TE, Xie Q, Fulton DB, Engen JR, Berg LJ, Andreotti AH. Sci Signal. 2013 Aug 27;6(290):ra76. doi: 10.1126/scisignal.2004298. PMID: 23982207 Free PMC Article Related citations Select item 23928097 Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications. Wei H, Mo J, Tao L, Russell RJ, Tymiak AA, Chen G, Iacob RE, Engen JR. Drug Discov Today. 2014 Jan;19(1):95-102. doi: 10.1016/j.drudis.2013.07.019. Epub 2013 Aug 6. Review. PMID: 23928097 Free PMC Article Related citations Select item 23795559 Active-site inhibitors modulate the dynamic properties of human monoacylglycerol lipase: a hydrogen exchange mass spectrometry study. Karageorgos I, Wales TE, Janero DR, Zvonok N, Vemuri VK, Engen JR, Makriyannis A. Biochemistry. 2013 Jul 23;52(29):5016-26. doi: 10.1021/bi400430k. Epub 2013 Jul 8. PMID: 23795559 Free PMC Article Related citations Select item 23747483 Insights into Notch3 activation and inhibition mediated by antibodies directed against its negative regulatory region. Tiyanont K, Wales TE, Siebel CW, Engen JR, Blacklow SC. J Mol Biol. 2013 Sep 9;425(17):3192-204. doi: 10.1016/j.jmb.2013.05.025. Epub 2013 Jun 6. PMID: 23747483 Free PMC Article Related citations