个人简介

B.S., Chemistry, Pacific University 2005 Ph. D., Biochemistry and Molecular Biology, Oregon Health and Science University 2009. Advisor: Dr. Pierre Moenne-Loccoz Postdoctoral, University of Minnesota, Advisor Dr. Carrie Wilmot Awards and Honors National Research Service Award, National Institutes of Health, 2011-2013 Boyer-Peter Award for Postdoctoral Research, University of Minnesota, 2013

研究领域

Biochemistry

Transition metals such as iron, copper, manganese and zinc serve essential functions in biology and are required for such fundamental processes as respiration, photosynthesis and DNA synthesis among others. My lab is interested in the mechanisms by which bacteria acquire these metals from the environment, and how certain metalloproteins are used as sensors of environmental conditions. These two processes are important for virulence and survival of pathogenic bacteria within the human host. We employ X-ray crystallography as well as various spectroscopic, biophysical, and molecular biology techniques to assess the structure, function and mechanism of proteins involved in these processes. The long-term goal of these studies is to further the development of improved antibiotics against resistant bacteria, which target these metal import and sensory functions.

近期论文

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Johnson, B. J., Yukl, E. T., Klema, V. J., Klinman, J. P., Wilmot, C. M. (2013) Structural snapshots from the oxidative half-reaction of a copper amine oxidase: implications for O2 activation. J. Biol. Chem. 288 (39), 28409-17. Tarboush, N. A., Yukl, E. T., Shin, S., Feng, M., Wilmot, C. M., Davidson, V. L., (2013) Carboxyl group of Glu113 is required for stabilization of the diferrous and bis-Fe(IV) states of MauG. Biochemistry 52 (37), 6358-67. Yukl, E. T., Jensen L. M., Davidson, V. L., Wilmot, C. M. (2013) Structures of MauG in complex with quinol and quinone MADH. Acta. Cryst. F. 69, 738-43. Yukl, E. T., Liu, F., Krzystek, J., Shin, S., Jensen, L. M. R., Davidson, V. L., Wilmot, C. M., Liu, A. (2013) A di-radical intermediate within the context of tryptophan tryptophylquinone biosynthesis. Proc. Natl. Acad. Sci. U. S. A.110 (12), 4569-73. Wilmot, C. M., Yukl, E. T. (2013) MauG: A di-heme enzyme required for methylamine dehydrogenase maturation. Dalton Trans. 42 (9), 3127-35. Yukl, E. T., Wilmot, C. M. (2012) Cofactor biosynthesis through protein post-translational modification. Curr. Opin. Chem. Biol. 16 (1-2), 54-9. Feng, M., Jensen, L. M., Yukl, E. T., Wei, X., Liu, A., Wilmot, C. M., Davidson, V. L. (2012) Proline 107 is a major determinant in maintaining the structure of the distal pocket and reactivity of the high-spin heme of MauG. Biochemistry 51 (8), 1598-606. Tarboush, N. A., Jensen, L. M., Yukl, E. T., Geng, J., Liu, A., Wilmot, C. M., Davidson, V. L. (2011) Mutagenesis of tryptophan199 suggests that hopping is required for MauG-dependent tryptophan tryptophylquinone biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 108 (41), 16956-61. Yukl, E. T., Goblirsch, B. R., Davidson, V. L., Wilmot, C. M. (2011) Crystal structures of the CO and NO adducts of MauG in complex with pre-methylamine dehydrogenase: Implications for the mechanism of dioxygen activation. Biochemistry 50 (14), 2931-8. Yukl, E. T., Ioanoviciu, A., Sivaramakrishnan, S., Nakano, M. M., Ortiz de Montellano, P. R., Moënne-Loccoz, P. (2011) Nitric oxide dioxygenation reaction in DevS and the initial response to nitric oxide in Mycobacterium tuberculosis. Biochemistry 50 (6), 1023-8. Kommineni, S., Yukl, E. T., Hayashi, T., Delepine, J., Geng, H., Moënne-Loccoz, P., Nakano, M. M. (2010) Nitric oxide-sensitive and –insensitive interaction of Bacillus subtillis NsrR with a ResDE-controlled promoter. Mol. Microbiol. 78 (5), 1280-93. Yukl, E. T., Jepkorir, G., Alontaga, A. Y., Pautsch, L., Rodriguez, J. C., Rivera, M., Moënne-Loccoz, P. (2010) Kinetic and spectroscopic studies of hemin acquisition in the hemophore HasAp from Pseudomonas aeruginosa. Biochemistry 49 (31), 6646-54. Jepkorir, G., Rodriguez, J. C., Rui, H., Im, W., Lovell, S., Battaile, K. P., Alontaga, A. Y., Yukl, E. T., Moënne-Loccoz, P., Rivera, M. (2010) Structural, NMR spectroscopic, and computational investigation of hemin loading in the hemophore HasAp from Pseudomonas aeruginosa. J. Am. Chem. Soc. 132 (28), 9857-72. Yukl, E. T., de Vries, S., Moënne-Loccoz, P. (2009) The millisecond intermediate in the reaction of nitric oxide with oxymyoglobin is an iron(III)-nitrato complex, not a peroxynitrite. J. Am. Chem. Soc. 131 (21), 7234-35. Alontaga, A. Y., Rodrigurez, J. C., Shönbrunn, E., Becker, A., Funke, R., Yukl, E. T., Hayashi, T., Stobaugh, J., Moënne-Loccoz, P., Rivera, M. (2009) Structural characterization of the hemophore HasAp from Pseudomonas aeruginosa: NMR spectroscopy reveals protein−protein interactions between holo-HasAp and hemoglobin. Biochemistry 48 (1) 96-109. Yukl, E. T., Elbaz, M. A., Nakano, M. M., Moënne-Loccoz, P. (2008) Transcription factor NsrR from Bacillus subtilis senses nitric oxide with a 4Fe−4S cluster. Biochemistry 47 (49), 13084-13092. Yukl, E. T., Ioanoviciu, A., Nakano, M. M., Ortiz de Montellano, P. R., Moënne-Loccoz, P. (2008). A distal tyrosine residue is required for ligand discrimination in DevS from Mycobacterium tuberculosis. Biochemistry 47 (47), 12532-9. Yukl, E. T., Ioanoviciu, A., de Montellano. P. R., Moënne-Loccoz, P. (2007). Interdomain interactions within the two-component heme-based sensor DevS from Mycobacterium tuberculosis. Biochemistry 46 (34), 9728-36. Ioanoviciu, A., Yukl, E. T., Moënne-Loccoz, P., de Montellano P. R. (2007). DevS, a heme-containing two-component oxygen sensor of Mycobacterium tuberculosis. Biochemistry 46 (14), 4250-60. Whittaker, M. M., Pan, H. Y., Yukl E. T., Whittaker, J. W. (2007). Burst kinetics and redox transformations of the active site manganese ion in oxalate oxidase: Implications for the catalytic mechanism. J. Biol. Chem. 282 (10), 7011-23.