Theg, Steven M. - University of California, Davis - College of Biological Sciences

研究领域

Protein transport Protein translocation across biological membranes, with a focus on chloroplast membranes. Mechanism of multimeric protein complex assembly. Chloroplast bioenergetics. The current objectives of the research in the Theg lab are to understand the events surrounding the transport of proteins across biological membranes and their assembly into larger multimeric complexes. Most of the laboratory's efforts focus on protein trafficking and assembly in chloroplasts isolated from higher plants (often peas, but also Arabidopsis and the moss Physcomitrella patens). These organelles represent an excellent model system in which to study cellular protein targeting as they are easily separated from other organelles, and retain a high level of metabolic activity after isolation. In addition, they contain a level of structural complexity that requires additional sorting of proteins within the organelle. In these sorting reactions, chloroplasts utilize different protein targeting paradigms which are individually used by other organelles. Consequently, lessons learned from the study of protein targeting to and within chloroplasts can, in principle, be applied to a number of other polypeptide translocating systems.

近期论文

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Ho, J. and Theg, S.M. (2015) The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana Benthamiana. PLOS ONE doi:10.1371/journal.pone.0146489 Wang, X, Wei, Y., Shi, L-X., Ma, X and Theg, S.M. (2015) New Isoforms and Assembly of Glutamine Synthetase in the Leaf of Wheat (Triticum aestivum L.). J. Exp. Bot. 66:6827-6834. doi: 10.1093/jxb/erv388 Liu, L., McNielage, R.T., Shi, L.-X. and Theg, S.M. (2014) ATP requirement for chloroplast protein import is set by the Km for ATP hydrolysis of the stromal Hsp70 in Physcomitrella patens. Plant Cell 26: 1246-1255. doi: 10.1105/tpc.113.121822 Bricker, T.M., Bell, A.J., Tran, L. Frankel, L.K. and Theg, S.M. (2013) Photoheterotrophic growth of Physcomitrella patens. Planta 293: 605-613. doi: 10.1007/s00425-013-2000-3 Shi, L-X. and Theg, S.M. (2013) The energetic cost of protein import across the envelope membranes of chloroplasts. Proc. Natl. Acad. Sci. USA, 110:930-935. Shi, L-X. and Theg, S.M. (2013) The chloroplast protein import system: From algae to trees. Biochim. Biophys. Acta 1833:314–331. Lo, S.M. and Theg, S.M. (2012) Role of Vesicle-Inducing Protein in Plastids 1 in cpTat Transport at the Thylakoid. The Plant J. 71:656-668. Shi, L-X. and Theg, S.M. (2011) The motors of protein import into chloroplasts. Plant Signaling & Behavior 6:1397-1401. Theg, S.M. and Tom, C. (2011) Measurement of the ΔpH and electric field developed across Arabidopsis thylakoids in the light. Methods Mol. Biol. 775:327-357. Shi, L-X. and Theg, S.M. (2010) A Stromal Hsp70 System Functions in Protein Import into Chloroplasts in the Moss Physcomitrella patens. The Plant Cell 22:1-16. Lo, S.M. and Theg, S.M.(2010) Protein Targeting Across and Into Chloroplast Membranes. Methods Mol. Biol. 684:139-157. Theg, S.M. (2010) Measurement of the Energetics of Protein Transport across the Chloroplast Thylakoid Membrane. Methods Mol. Biol. 619:323-337.