个人简介

Education Ph. D., University of California at Davis (1996) Postdoctoral Fellow: University of Pennsylvania

研究领域

Biophysical, magnetic resonance spectroscopy

The research in the Lorigan laboratory is focused upon the utilization of magnetic resonance spectroscopy to investigate the structural and dynamic properties of membrane-bound proteins and peptides and their interactions within the lipid bilayer. We are specifically interested in developing and applying state-of-the-art nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopic techniques to study membrane proteins inserted into aligned phospholipid bilayers. Membrane proteins (which make up approximately one-third of the total number of proteins) are responsible for many important properties and functions of biological systems: they transport ions and molecules across the membrane, they act as receptors, and they have roles in the assembly, fusion, and structure of cells and viruses. Despite the abundance and clear importance of these membrane proteins and peptides, only a minute amount of information regarding these systems exists. High-resolution multi-nuclear solid-state NMR spectroscopy is a powerful technique that can yield pertinent structural, orientational, and dynamic information about membrane protein systems when they are aligned with the direction of the static magnetic field. State-of the-art EPR spectroscopy coupled with site-specific nitroxide spin labeling can determine motional parameters and measure the distances between spin labels separated from 8 to 70 Å.

近期论文

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Conformational dynamics of metallo-β-lactamase CcrA during catalysis investigated by using DEER spectroscopy. Aitha M, Moritz L, Sahu ID, Sanyurah O, Roche Z, McCarrick R, Lorigan GA, Bennett B, Crowder MW. J Biol Inorg Chem. 2015 Apr;20(3):585-94. doi: 10.1007/s00775-015-1244-8. Epub 2015 Feb 10. PMID: 25827593 Related citations Select item 25462949 CW dipolar broadening EPR spectroscopy and mechanically aligned bilayers used to measure distance and relative orientation between two TOAC spin labels on an antimicrobial peptide. Sahu ID, Hustedt EJ, Ghimire H, Inbaraj JJ, McCarrick RM, Lorigan GA. J Magn Reson. 2014 Oct 22;249C:72-79. doi: 10.1016/j.jmr.2014.09.020. [Epub ahead of print] PMID: 25462949 Related citations Select item 25234231 Structural investigation of the transmembrane domain of KCNE1 in proteoliposomes. Sahu ID, Kroncke BM, Zhang R, Dunagan MM, Smith HJ, Craig A, McCarrick RM, Sanders CR, Lorigan GA. Biochemistry. 2014 Oct 14;53(40):6392-401. doi: 10.1021/bi500943p. Epub 2014 Oct 3. PMID: 25234231 Related citations Select item 24853657 Characterizing the structure of lipodisq nanoparticles for membrane protein spectroscopic studies. Zhang R, Sahu ID, Liu L, Osatuke A, Comer RG, Dabney-Smith C, Lorigan GA. Biochim Biophys Acta. 2015 Jan;1848(1 Pt B):329-33. doi: 10.1016/j.bbamem.2014.05.008. Epub 2014 May 20. PMID: 24853657 Related citations Select item 24689497 Solid-state NMR (31)P paramagnetic relaxation enhancement membrane protein immersion depth measurements. Maltsev S, Hudson SM, Sahu ID, Liu L, Lorigan GA. J Phys Chem B. 2014 Apr 24;118(16):4370-7. doi: 10.1021/jp500267y. Epub 2014 Apr 11. PMID: 24689497 Free PMC Article Related citations Select item 24511878 Secondary structure, backbone dynamics, and structural topology of phospholamban and its phosphorylated and Arg9Cys-mutated forms in phospholipid bilayers utilizing 13C and 15N solid-state NMR spectroscopy. Yu X, Lorigan GA. J Phys Chem B. 2014 Feb 27;118(8):2124-33. doi: 10.1021/jp500316s. Epub 2014 Feb 18. PMID: 24511878 Free PMC Article Related citations Select item 24144541 Investigating the interaction between peptides of the amphipathic helix of Hcf106 and the phospholipid bilayer by solid-state NMR spectroscopy. Zhang L, Liu L, Maltsev S, Lorigan GA, Dabney-Smith C. Biochim Biophys Acta. 2014 Jan;1838(1 Pt B):413-8. doi: 10.1016/j.bbamem.2013.10.007. Epub 2013 Oct 19. PMID: 24144541 Free PMC Article Related citations Select item 24075840 Solid-state NMR investigations of peptide-lipid interactions of the transmembrane domain of a plant-derived protein, Hcf106. Zhang L, Liu L, Maltsev S, Lorigan GA, Dabney-Smith C. Chem Phys Lipids. 2013 Oct-Nov;175-176:123-30. doi: 10.1016/j.chemphyslip.2013.09.002. Epub 2013 Sep 25. PMID: 24075840 Related citations Select item 24055473 NMR structure and MD simulations of the AAA protease intermembrane space domain indicates peripheral membrane localization within the hexaoligomer. Ramelot TA, Yang Y, Sahu ID, Lee HW, Xiao R, Lorigan GA, Montelione GT, Kennedy MA. FEBS Lett. 2013 Nov 1;587(21):3522-8. doi: 10.1016/j.febslet.2013.09.009. Epub 2013 Sep 18. PMID: 24055473 Free PMC Article Related citations Select item 23984855 DEER EPR measurements for membrane protein structures via bifunctional spin labels and lipodisq nanoparticles. Sahu ID, McCarrick RM, Troxel KR, Zhang R, Smith HJ, Dunagan MM, Swartz MS, Rajan PV, Kroncke BM, Sanders CR, Lorigan GA. Biochemistry. 2013 Sep 24;52(38):6627-32. doi: 10.1021/bi4009984. Epub 2013 Sep 9. PMID: 23984855 Free PMC Article Related citations Select item 23961941 Use of electron paramagnetic resonance to solve biochemical problems. Sahu ID, McCarrick RM, Lorigan GA. Biochemistry. 2013 Sep 3;52(35):5967-84. doi: 10.1021/bi400834a. Epub 2013 Aug 20. Review. PMID: 23961941 Free PMC Article Related citations Select item 23850636 Probing the interaction of Arg9Cys mutated phospholamban with phospholipid bilayers by solid-state NMR spectroscopy. Yu X, Lorigan GA. Biochim Biophys Acta. 2013 Nov;1828(11):2444-9. doi: 10.1016/j.bbamem.2013.07.003. Epub 2013 Jul 10. PMID: 23850636 Free PMC Article Related citations Select item 22966895 Determining α-helical and β-sheet secondary structures via pulsed electron spin resonance spectroscopy. Zhou A, Abu-Baker S, Sahu ID, Liu L, McCarrick RM, Dabney-Smith C, Lorigan GA. Biochemistry. 2012 Sep 25;51(38):7417-9. doi: 10.1021/bi3010736. Epub 2012 Sep 14. PMID: 22966895 Free PMC Article Related citations Select item 22908896 Enhancement of electron spin echo envelope modulation spectroscopic methods to investigate the secondary structure of membrane proteins. Liu L, Sahu ID, Mayo DJ, McCarrick RM, Troxel K, Zhou A, Shockley E, Lorigan GA. J Phys Chem B. 2012 Sep 13;116(36):11041-5. doi: 10.1021/jp304669b. Epub 2012 Aug 30. PMID: 22908896 Free PMC Article Related citations Select item 22558151 The influence of spin-labeled fluorene compounds on the assembly and toxicity of the aβ peptide. Petrlova J, Kálai T, Maezawa I, Altman R, Harishchandra G, Hong HS, Bricarello DA, Parikh AN, Lorigan GA, Jin LW, Hideg K, Voss JC. PLoS One. 2012;7(4):e35443. doi: 10.1371/journal.pone.0035443. Epub 2012 Apr 30. PMID: 22558151 Free PMC Article Related citations Select item 22397695 Differential binding between PatS C-terminal peptide fragments and HetR from Anabaena sp. PCC 7120. Feldmann EA, Ni S, Sahu ID, Mishler CH, Levengood JD, Kushnir Y, McCarrick RM, Lorigan GA, Tolbert BS, Callahan SM, Kennedy MA. Biochemistry. 2012 Mar 27;51(12):2436-42. doi: 10.1021/bi300228n. Epub 2012 Mar 15. PMID: 22397695 Related citations Select item 22379959 Distance measurements on a dual-labeled TOAC AChR M2δ peptide in mechanically aligned DMPC bilayers via dipolar broadening CW-EPR spectroscopy. Ghimire H, Hustedt EJ, Sahu ID, Inbaraj JJ, McCarrick R, Mayo DJ, Benedikt MR, Lee RT, Grosser SM, Lorigan GA. J Phys Chem B. 2012 Mar 29;116(12):3866-73. doi: 10.1021/jp212272d. Epub 2012 Mar 19. PMID: 22379959 Free PMC Article Related citations Select item 22172806 Probing the helical tilt and dynamic properties of membrane-bound phospholamban in magnetically aligned bicelles using electron paramagnetic resonance spectroscopy. Ghimire H, Abu-Baker S, Sahu ID, Zhou A, Mayo DJ, Lee RT, Lorigan GA. Biochim Biophys Acta. 2012 Mar;1818(3):645-50. doi: 10.1016/j.bbamem.2011.11.030. Epub 2011 Dec 4. PMID: 22172806 Free PMC Article Related citations Select item 22100865 Time-resolved EPR immersion depth studies of a transmembrane peptide incorporated into bicelles. Nusair NA, Mayo DJ, Dorozenski TD, Cardon TB, Inbaraj JJ, Karp ES, Newstadt JP, Grosser SM, Lorigan GA. Biochim Biophys Acta. 2012 Mar;1818(3):821-8. doi: 10.1016/j.bbamem.2011.11.009. Epub 2011 Nov 11. PMID: 22100865 Free PMC Article Related citations Select item 22085289 Reconstitution of KCNE1 into lipid bilayers: comparing the structural, dynamic, and activity differences in micelle and vesicle environments. Coey AT, Sahu ID, Gunasekera TS, Troxel KR, Hawn JM, Swartz MS, Wickenheiser MR, Reid RJ, Welch RC, Vanoye CG, Kang C, Sanders CR, Lorigan GA. Biochemistry. 2011 Dec 20;50(50):10851-9. doi: 10.1021/bi2009294. Epub 2011 Nov 22. PMID: 22085289 Free PMC Article