Callis, Judy - University of California, Davis - College of Biological Sciences

研究领域

My laboratory studies two processes. The first is the ubiquitin pathway, which is a protein modification pathway, whereby the protein ubiquitin is covalently attached to other proteins. This alters the longevity, activity or localization of the ubiquitinated protein. We are interested in understanding the specificity and regulation of the ubiquitin pathway. We try to understand how proteins are recruited to the ubiquitinating enzymes. There are are large number of ubiquitinating enzymes whose functions are not known. We are taking a reverse genetics approach to understand the function of RING E3 ligases which interact with substrate proteins and catalyze ubiquitin transfer. We use a variety of techniques- biochemistry, molecular biology and genetics. We also study a family of proteins called pfkB proteins, proteins related to E. coli phosphofructokinase, but which have diverse and uncharacterized roles. We are focusing on a subset of pfkB proteins localized to the plant plastid. Some of these are important for chloroplast transcription and we are determining their functions.

近期论文

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Gilkerson J, Kelley DR, Tam R, Estelle M, Callis J. 2015 Lysine residues are not required for proteasome-mediated proteolysis of the auxin/IAA protein IAA1. Plant Physiol., 168: 708-720. Callis J. 2014 The Ubiquitination Machinery of the Ubiquitin System, The Arabidopsis Book, invited book chapter, 12: e0174. Chen Y-T, Liu H, Stone S, Callis J. 2013 ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3. Plant J. 75: 965–976. Guerra D, Pratelli R, Kraft E, Callis J, Pilot G. 2013 Functional conservation between mammalian MGRN1 and plant LOG2 ubiquitin ligases. FEBS Letters, 587: 3400-3405. Shin L-J, Lo J, Chen G-H, Callis J, Fu H-Y, Yeh, K-C. 2013 IRT1 DEGRADATION FACTOR 1, a RING E3 ubiquitin ligase, regulates the degradation of IRON REGULATED TRANSPORTER 1 in Arabidopsis. Plant Cell 2013 25: 3039-3051. Réjane Pratelli, Damian D. Guerra, Shi Yu, Mark Wogulis, Edward Kraft, Wolf B. Frommer, Judy Callis and Guillaume Pilot 2012 The Ubiquitin E3 Ligase LOSS OF GDU2 Is Required for GLUTAMINE DUMPER1-Induced Amino Acid Secretion in Arabidopsi. Plant Physiology 158:1628-1642. Guerra D, Callis J. 2012 Ubiquitin on the Move: the ubiquitn modification pathway plays diverse roles in the regulation of ER-and membrane-localized proteins Plant Physiol. 160: 56-64. Hotton SK, Castro MF, Eigenheer RA, Callis J. 2012 Recovery of DDB1a (DAMAGED DNA BINDING PROTEIN1a) in a Screen to Identify Novel RUB-Modified Proteins in Arabidopsis thaliana. Molecular Plant 5: 1163-1166. Gilkerson J, Perez-Ruiz JM, Chory J, Callis J. 2012 The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana. BMC Plant Biology 12. Hotton, SK., Eigenheer, RA., Castro, MF., Bostick M., and Callis, J. 2011 AXR1-ECR1 and AXL1-ECR1 heterodimeric RUB-activating enzymes diverge in function in Arabidopsis thaliana Plant Mol. Biology, 75: 515-526 Hsia, MM. and Callis, J. 2010 BRIZ1 and BRIZ2 proteins form a heteromeric E3 ligase complex required for seed germination and post-germination growth in Arabidopsis thaliana. J. Biol. Chem., 285:37070-37081 Berrocal-Lobo, M, Stone, S, Yang, X, Antico, J, Callis, J, Ramonell, KM, Somerville, S. 2010 ATL9, a RING Zinc Finger Protein with E3 Ubiquitin Ligase Activity Implicated in Chitin- and NADPH Oxidase-Mediated Defense Responses. Plos One vol 5, issue 12. 10.1371/journal.pone.0014426 Gilkerson, J. Hu, J. Brown, J. Jones, J. Sun, T-P. and Callis J. 2009 Isolation and Characterization of cul1-7, a Recessive Allele of CULLIN1 that Disrupts SCF Function at the C-terminus of CUL1 in Arabidopsis thaliana Genetics, 181:945-963 Jowett, J., Ramos, J. and Callis, J. 2008 Degradation of auxin response factor ARF1 Plant J. 54: 118-128 Hotton, S. and Callis, J 2008 Regulation of Cullin-based E3 ligases Ann. Rev. Plant Biol. 59: 469-489 Kraft, E., Bostick, M., Jacobsen, S., and Callis, J. 2008 ORTH/VIM proteins that regulate DNA methylation are functional ubiquitin E3 ligases. Plant J. 56: 704-715 Dreher, K.A. and Callis, J. 2007 Ubiquitin, Hormones and Biotic Stress in Plants. Annals of Bot. 9: 787 - 822. Stone SL, Callis J. 2007 Ubiquitin ligases mediate growth and development by promoting protein death. Curr Opin Plant Biol. Sep 10; PMID: 17851112 Doelling JH, Phillips AR, Soyler-Ogretim G, Wise J, Chandler J, Callis J, Otegui MS, Vierstra RD 2007 The Ubiquitin-Specific Protease Subfamily UBP3/UBP4 Is Essential for Pollen Development and Transmission in Arabidopsis. Plant Physiol. PMID: 17905865 Scherer GF, Zahn M, Callis J, Jones AM. A role for phospholipase A in auxin-regulated gene expression. FEBS Lett. 2007 581:4205-11 Johnson, L.M., Bostick, M. Zhang, X, Kraft, E., Henderson, I., Callis, J. and Jacobsen, S.E. 2007 The SRA Methyl-Cytosine-Binding Domain Links DNA and Histone Methylation. Current Biology 17, 1-6 Stone, S.L., Williams, L.A., Farmer, L.M., Vierstra, R.D. and Callis, J. 2006 KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling. Plant Cell 18, 3415-3428 Dreher, K., Brown, J. Saw, R. and Callis, J. 2006 The Arabidopsis Aux/IAA Protein Family Has Diversified in Degradation and Auxin Responsiveness. The Plant Cell, 18, 699-714. Callis, J. 2005 Plant biology: Auxin action (Invited commentary) Nature 435, 436-437 Kraft, E., Stone, S., Ma, L., Su, N., Gao, Y., Lau, O., Deng, X-W, and Callis, J. 2005 Genome Analysis and Functional Characterization of the E2 and RING domain E3 Ligase Ubiquitination Enzymes of Arabidopsis thaliana Plant Physiol., 139, 1597-1611.