Zhang, Wenjun - University of California, Berkeley - Chemical & Biomolecular Engineering

个人简介

B.S., Biochemistry, Nanjing University (2002) M.S., Biochemistry, Nanjing University (2004) Ph.D., Chemical Engineering, University of California, Los Angeles (2009) Postdoctoral Research Fellow, Harvard Medical School (2009-2011)

研究领域

Biomolecular Engineering Our lab is interested in biomolecular engineering for applications related to human health and bioenergy. Microbial cells have been programmed to possess exquisite catalytic power in the synthesis of a large array of important natural products. Recent advances in understanding of these biosynthetic machineries have provided unprecedented opportunities to biosynthesize target molecules including pharmaceuticals and biofuels. Our research theme includes understanding and engineering the biosynthesis of natural products, new bioactive small molecule discovery and functional study, and development of new research toolkit to promote the natural product research with a particular focus on natural product tagging and applications.

近期论文

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Herman, N., Li, J., Bedi, R., Turchi, B., Liu, X., Miller, M., Zhang, W. "Development of a high-efficiency transformation method and implementation of rational metabolic engineering for the industrial butanol hyper-producer Clostridium saccharoperbutylacetonicum strain N1-4" Appl. Environ. Microbiol. 2016, accepted. Link Herman, N., Zhang, W. "Enzymes for fatty-acid based hydrocarbon biosynthesis" Curr. Opin. Chem. Biol. 2016, in press. Link Zhu, X., Shieh, P., Su, M., Bertozzi, C., Zhang, W. "A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool" ChemComm. 2016. Link Liu, J., Zhu, X., Kim, S., Zhang, W. "Antimycin-type depsipeptides: discovery, biosynthesis, chemical synthesis, and bioactivities" Nat. Prod. Rep. 2016, 33, 1146-1165 Link Rui, Z., Zhang, W. "Combining genome mining and biosynthesis research to discover bioactive natural products and new targets for medicinal chemistry" Curr. Top. Med. Chem. 2016, 16, 1643-1644. (Editorial) Link Rui, Z., Zhang, W."Engineering biosynthesis of non-ribosomal peptides and polyketides by directed evolution" Curr. Top. Med. Chem. 2016, 16, 1755-1762 Link Liu, J., Zhang, W. "Recent advances in understanding and engineering polyketide synthesis." F1000 Faculty Rev. 2016, 5. Link Rui, Z., Harris, N. C., Zhu, X., Huang, W., Zhang, W. "Discovery of a family of desaturase-like enzymes for 1-alkene biosynthesis." ACS Catal. 2015, 5, 7091-7094. Link Huang, W., Kim, S., Liu, J., Zhang, W. "Identification of the polyketide biosynthetic machinery for the indolizidine alkaloid cyclizidine." Org. Lett. 2015, 17, 5344-5347. Link Liu, J., Zhu, X., Zhang, W. "Identifying the minimal enzymes required for biosynthesis of epoxyketone proteasome inhibitors." ChemBioChem. 2015, 16, 2585-2589. Link Zhu, X., Su, M., Manickam, K., Zhang, W. "Bacterial genome mining of enzymatic tools for alkyne biosynthesis." ACS Chem. Biol. 2015, 10, 2785-2793. Link Rui, Z., Huang, W., Xu, F., Han, M., Liu, X., Lin, S., Zhang, W. "Sparsomycin biosynthesis highlights unusual module architecture and processing mechanism in non-ribosomal peptide synthetase." ACS Chem. Biol. 2015, 10, 1765-1769. Link Zhu, X., Zhang, W. "Tagging polyketides/non-ribosomal peptides with a clickable functionality and applications." Front. Chem. 2015, 3: 11. Link Zhu, X., Liu, J., Zhang, W. "De novo biosynthesis of terminal alkyne-labeled natural products." Nat. Chem. Biol. 2015, 11, 115-120. Link News and Views NCB's Greatest hits Rui, Z., Li, X., Zhu, X., Liu, J., Domigan, B., Barr, I., Cate, J. H. D., Zhang, W. "Microbial biosynthesis of medium-chain 1-alkenes by a non-heme iron oxidase." Proc. Natl. Acad. Sci. U. S. A. 2014, 111, 18237-18242. Link Highlight in Angew. Chem. Liu, J., Zhu, X., Seipke, R. F., Zhang, W. “Biosynthesis of antimycins with a reconstituted 3-formamidosalicylate pharmacophore in Escherichia coli.” ACS Synth. Biol. 2015, 4, 559-565. Link Liu, J., Ng, T., Rui, Z., Ad, O., Zhang, W. “Unusual acetylation-dependent reaction cascade in the biosynthesis of the pyrroloindole drug physostigmine.” Angew. Chem. Int. Ed. Engl. 2014, 53, 136-139. Link Sandy, M., Zhu, X., Rui, Z., Zhang, W. “Characterization of AntB, a promiscuous acyltransferase involved in antimycin biosynthesis.” Org. Lett. 2013, 15, 3396-3399. Link Rui, Z., Sandy, M., Jung, B., Zhang, W. "Tandem enzymatic oxygenations in biosynthesis of epoxyquinone pharmacophore of manumycin-type metabolites." Chem. Biol. 2013, 20, 859-860. Link Sandy, M., Rui, Z., Gallagher, J., Zhang, W. “Enzymatic synthesis of dilactone scaffold of antimycins.” ACS Chem. Biol. 2012, 7, 1956-1961. Link Rui, Z., Ye, M., Wang, S., Fujikawa, K., Akerele, B., Aung, M., Floss, H., Zhang, W., Yu, T. “Insights into a divergent phenazine biosynthetic pathway governed by a plasmid-born esmeraldin gene cluster.” Chem. Biol. 2012, 19, 1116-1125. Link Walsh, C. T., Zhang, W. “Chemical logic and enzymatic machinery for biological assembly of peptidyl nucleoside antibiotics.” ACS Chem. Bio. 2011, 6, 1000-1007. Link Ames, B. D., Lee, M. Y., Moody, C. L., Zhang, W., Tang, Y., Tsai, S. C. “Structural and biochemical characterization of ZhuI aromatase/cyclase from the R1128 polyketide pathway.” Biochemistry 2011, 50, 8392-8406. Link Zhang, W., Ntai, I., Kelleher, N. L., Walsh, C. T. “tRNA-dependent peptide bond formation by the transferase PacB in biosynthesis of the pacidamycin group of pentapeptidyl nucleoside antibiotics.” Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 12249-12253. Link Zhang, W., Ames, B. D., Walsh, C. T. “Identification of phenylalanine 3-hydroxylase for meta-tyrosine biosynthesis.” Biochemistry 2011, 50, 5401-5403. Link