研究领域

Biochemistry and biophysical chemistry of membrane proteins

Our broad interests lie in addressing the fundamental questions surrounding the interactions of lipid bilayers with proteins. Using various biophysical techniques namely NMR and analytical ultracentrifugation, we aim to understand the behavior of proteins in the lipid milieu on a molecular level. Our research capitalizes on the use of bicelles, a "native-like" membrane mimic which has great potential to overcome some of the hurdles that have limited the study of membrane proteins.

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Rui, H., Root, K. T., Lee, J., Glover, K. J., and Im, W. (2014) Probing the U-shaped Confomation of Caveolin-1 in a Bilayer. Biophys J. 106, 1371-1380. Rieth, M. D., Lee, J., and Glover, K. J. (2012) Probing the Caveolin-1 P132L Mutant: Critical Insights into its Oligomeric Behavior and Structure. Biochemistry. 51, 3911-3918. Lee, J., and Glover, K. J. (2012) The Transmembrane Domain of Caveolin-1 Exhibits a Helix-Break-Helix Structure. Biochim. Biophys. Acta. 1818, 1158-1164. Mohanty, P., Lee, J., Glover, K. J., and Landskron, K. (2010) Discoid Bicelles as Efficient Templates for Pillared Lamellar Periodic Mesoporous Silicas at pH 7 and Ultrafast Reaction Times. Nanoscale Res. Lett. 6, 61-010-9813-9. Diefenderfer, C., Lee, J., Mlyanarski, S., Guo, Y., and Glover, K. J. (2009) Reliable Expression and Purification of Highly Insoluble Transmembrane Domains. Anal. Biochem. 384, 274-278. Zhang, J., Li, D., Liu, G., Glover, K. J., and Liu, T. (2009) Lag Periods during the Self-Assembly of {Mo(72)Fe(30)} Macroions: Connection to the Virus Capsid Formation Process. J. Am. Chem. Soc.131, 15152-15159. Chen, M. M., Glover, K. J., and Imperiali, B. (2007) From Peptide to Protein: Comparative Analysis of the Substrate Specificity of N-Linked Glycosylation in C. Jejuni. Biochemistry. 46, 5579-5585. Glover, K. J., Weerapana, E., Chen, M. M., and Imperiali, B. (2006) Direct Biochemical Evidence for the Utilization of UDP-Bacillosamine by PglC, an Essential Glycosyl-1-Phosphate Transferase in the Campylobacter Jejuni N-Linked Glycosylation Pathway. Biochemistry. 45, 5343-5350. Glover, K. J., Weerapana, E., and Imperiali, B. (2005) In Vitro Assembly of the Undecaprenylpyrophosphate-Linked Heptasaccharide for Prokaryotic N-Linked Glycosylation. Proc. Natl. Acad. Sci. U. S. A. 102, 14255-14259. Glover, K. J., Weerapana, E., Numao, S., and Imperiali, B. (2005) Chemoenzymatic Synthesis of Glycopeptides with PglB, a Bacterial Oligosaccharyl Transferase from Campylobacter Jejuni. Chem. Biol. 12, 1311-1315. Weerapana, E., Glover, K. J., Chen, M. M., and Imperiali, B. (2005) Investigating Bacterial N-Linked Glycosylation: Synthesis and Glycosyl Acceptor Activity of the Undecaprenyl Pyrophosphate-Linked Bacillosamine. J. Am. Chem. Soc. 127, 13766-13767. Whiles, J. A., Glover, K. J., Vold, R. R., and Komives, E. A. (2002) Methods for Studying Transmembrane Peptides in Bicelles: Consequences of Hydrophobic Mismatch and Peptide Sequence. J. Magn. Reson. 158, 149-156. Glover, K. J., Whiles, J. A., Vold, R. R., and Melacini, G. (2002) Position of Residues in Transmembrane Peptides with Respect to the Lipid Bilayer: A Combined Lipid Noes and Water Chemical Exchange Approach in Phospholipid Bicelles. J. Biomol. NMR. 22, 57-64. Glover, K. J., Whiles, J. A., Wood, M. J., Melacini, G., Komives, E. A., and Vold, R. R. (2001) Conformational Dimorphism and Transmembrane Orientation of Prion Protein Residues 110-136 in Bicelles. Biochemistry. 40, 13137-13142. Glover, K. J., Whiles, J. A., Wu, G., Yu, N., Deems, R., Struppe, J. O., Stark, R. E., Komives, E. A., and Vold, R. R. (2001) Structural Evaluation of Phospholipid Bicelles for Solution-State Studies of Membrane-Associated Biomolecules. Biophys. J. 81, 2163-2171. Whiles, J. A., Brasseur, R., Glover, K. J., Melacini, G., Komives, E. A., and Vold, R. R. (2001) Orientation and Effects of Mastoparan X on Phospholipid Bicelles. Biophys. J. 80, 280-293. Glover, K. J., Martini, P. M., Vold, R. R., and Komives, E. A. (1999) Preparation of Insoluble Transmembrane Peptides: Glycophorin-A, Prion (110-137), and FGFR (368-397). Anal. Biochem. 272, 270-274. Karpishin, T. B., Vannelli, T. A., and Glover, K. J. (1997) Copper-Driven Assembly of a Helical-Peptide-Strapped Zinc Porphyrin. J Am Chem Soc J Am Chem Soc. 119, 9063-9064.