Jayasinghe, Sajith - California State University, San Marcos - Department of Chemistry and Biochemistry

个人简介

Dr. Jayasinghe joined the Chemistry & Biochemistry department in the fall of 2005 after completing postdoctoral training at the University of Southern California. At CSUSM, Dr. Jayasinghe teaches classes in biochemistry (CHEM 341: General Biochemistry, CHEM 450: Protein Structure and Function), general chemistry (CHEM 105: General, Organic, and Biochemistry), as well as chemistry for general education (CHEM 315: Science in Film and TV). Dr. Jayasinghe's research involves the use of biochemical and biophysical techniques to investigate the structure and membrane association of polypeptides. At the present time, the main research projects in the laboratory involve attempts to elucidate the structure and function of several proteins (CsgA, CsgB, CsgE, CsgF, and CsgG) involved in the formation of bacterial curli. Postdoctoral Training: 2001-2005, Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles. Postdoctoral Training: 1999-2001, Department of Physiology and Biophysics, University of California, Irvine. Ph.D., Chemistry: 1993-1998, Unviersity of Virginia, Charlottesville. B.A., Chemistry: 1989-1993, Ohio Wesleyan University, Delaware, OH.

研究领域

In a broad sense, my research involves the use of biochemical and biophysical techniques to investigate the structure and membrane association of polypeptides. At the present time, my research involves three main areas of investigation: Determining the structure of thin aggregative fimbriae (curli) of salmonella. Investigating the sequence and structural characteristics that govern the aggregation of islet amyloid polypeptide (IAPP). Determining the conformation changes associated with the structural inter-conversion and membrane association of human C-reactive protein (hCRP).

近期论文

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Ritzel, R.A., Jayasinghe, S., Hansen, J.B., Sturis, J., Langen, R., and Butler, P.C., (2010), Beta-cell selective K(ATP)-channel activation protects beta cells and human islets from human islet amyloid polypeptide induced toxicity. Regulatory Peptides, 165(2-3): 158-162. Bedrood, S., Jayasinghe, S., Sieburth, D., Chen, M., Erbel, S., Butler, P.C.,Langen, R., and Ritzel, R.A., (2009), Annexin A5 directly interacts with amyloidogenic peoteins and reduces their toxicity. Biochemisty, 48(44):10568-76. Snider, C., Jayasinghe, S.A., Hristova, K., White, S.H., (2009), Membrane Protein Explorer: A Tool for Exploring Membrane Protein Topology. Protein Science,18(12):2624-8. Apostolidou, M., Jayasinghe, S.A., Langen, R., (2008), Structure of helical membrane bound IAPP and its implications for membrane-mediated misfolding. Journal of Biological Chemistry, 283(25):17205-17210. Jayasinghe S.A. and Langen, R., (2007), Membrane interaction of islet amyloid polypeptide. Biochimica et. Biophysica Acta. 1768(8): 2002-2009. Fernandez-Vidal, M., Jayasinghe, S., Ladokhin, A.S., White, S.H., (2007),Folding amphipathic helices into membranes: amphiphilicity trumps hydrophobicity. Journal of Molecular Biology, 370: 459-470. Meier, J.J., Kayed, R., Lin, C.Y., Gurlo, T., Haataja, L., Jayasinghe, S.,Langen, R., Glabe, C.C., Butler, P.C., (2006), Inhibition of hIAPP fibril formation does not prevent beta-cell death: Evidence for distinct actions of oligomers and fibrils of hIAPP. American Journal of Physiology - Endocrinology and Metabolism, 291(6): 1217-1324. Jayasinghe, S.A., and Langen R., (2005). Lipid Membranes Modulate the Structure of Islet Amyloid Polypeptide. Biochemistry, 44: 12113-12119. Jayasinghe, S.A., and Langen R., (2004). Identifying structural features of fibrillar islet amyloid polypeptide using site-directed spin labeling. Journal of Biological Chemistry, 279:48420-48425. Ellena, J.F., Moulthrop J., Wu, J., Rauch, M., Jayasinghe, S., Castle, J.D.,and Cafiso, D.S., (2004). Membrane position of a basic aromatic peptide that sequesters phosphatidylinositol 4,5 bisphosphate determined by site directed spin labeling and high-resolution NMR. Biophysical Journal, 87:3221-3233. Isas, J.M., Patel, D.R., Jao, C., Jayasinghe, S., Cartailler, J.P., Haigler,H.T., and Langen, R.(2003). Global structural changes in annexin 12- The role of phospholipids, Ca2+, and pH. Journal of Biological Chemistry,278:30227-30234. Jayasinghe, S., Hristova, K., and White, S.H. (2001). Transmembrane helix energetics and prediction accuracy. Journal of Molecular Biology, 312:927-934. Jayasinghe, S., Hristova, K., and White, S.H. (2001). MPtopo: A database of membrane protein topology. Protein Science, 10:455-458.