个人简介

MS, Eotvos Lorand University, Budapest, Hungary, 1999–2004 PhD, Chemistry, The George Washington University, 2005–2009 Postdoctoral Researcher, University of Illinois–Urbana-Champaign, 2009–2011 Staff Fellow and Laboratory Leader, US Food and Drug Administration­, 2011–2013

研究领域

Bioanalytical chemistry, mass spectrometry, and chemical separations for volume-limited samples; ambient mass spectrometry for food and drug regulation

Characterization of biomolecular events is pivotal to understanding states of health and disease and developing efficient therapeutic agents. The success of these investigations largely depends on the availability of sophisticated analytical toolsets, especially when samples are present in small amounts and high chemical complexity. Research in the Nemes Group develops analytical advances to resolve the biomolecular composition of single cells/neurons, organs, and tissues in model organisms. Microscale sampling and high-capacity separation are introduced to measure biomarkers such as metabolites, peptides, and proteins in complex biochemical milieus. Specifically, capillary electrophoresis for volume-limited investigations and ultraperformance liquid chromatography for high-throughput analyses are developed and coupled to mass spectrometry so that complementary types of compounds can be detected, identified, and quantified. These platforms are used to study mechanisms of action during development of conditions such as obesity and neurodegeneration as well as interactions with environmental factors. Our work recruits high-profile and emerging model organisms in biology, Caenorhabditis elegans (free living round worm) and Danio rerio (zebra fish), which share many features that are evolutionarily conserved in higher-order mammalian systems. The bioanalytical insights gained during these studies are in turn used to address mechanisms responsible for disease development and treatment opportunities in humans.

近期论文

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R. Shroff, K. Schramm, P. Nemes, A. Vertes, J. Gershenzon, and A. Svatos, Quantification of plant surface metabolites by MALDI mass spectrometry imaging: glucosinolates on Arabidopsis thaliana leaves, The Plant Journal 2014, in press E. J. Lanni, S. J. B. Dunham, P. Nemes, S. S. Rubakhin, J. V. Sweedler, Biomolecular imaging with a C60-SIMS/MALDI dual ion source hybrid mass spectrometer: Instrumentation, matrix enhancement and single cell analysis, J. Am. Soc. Mass Spectrom. 2014, in press, DOI: 10.1007/s13361-014-0978-9 H. Li, B. K. Smith, L. Mark, P. Nemes, J. Nazarian, and A. Vertes, Ambient molecular imaging by laser ablation electrospray ionization mass spectrometry with ion mobility separation, Int. J. Mass Spectrom. 2014, in press, DOI: 10.1016/j.ijms.2014.06.025 P. Nemes, W. J. Hoover, and D. A. Keire, High-throughput differentiation of heparin from other glycosaminoglycans by pyrolysis mass spectrometry, Anal. Chem. 2013, 85, 7405–7412, DOI: 10.1021/ac401318q P. Nemes, S. S. Rubakhin, J. Aerts, and J. V. Sweedler, Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry, Nat. Prot. 2013, 8, 783–799, DOI: 10.1038/nprot.2013.035 A. M. Knolhoff, K. M. Nautiyal, P. Nemes, S. Kalachikov, I. Morozova, R. Silver, and J. V. Sweedler, Combining small-volume metabolomic and transcriptomic approaches for assessing brain chemistry, Anal. Chem. 2013, 85, 3136–3143, DOI: dx.doi.org/10.1021/ac3032959 A. M. Knolhoff, P. Nemes, S. S. Rubakhin, and J. V. Sweedler, MS-based methodologies for single-cell metabolite detection and identification, in Methodologies for Metabolomics 2013, pp. 119–139, Experimental Strategies and Techniques, Eds. R. Wevers, N. Lutz, and J. V. Sweedler, Cambridge University Press A. Vertes, B. Shrestha, and P. Nemes, Direct metabolomics from tissues and cells: new approaches for small molecule and lipid characterization, in Methodologies for Metabolomics 2013, pp. 140–158, Experimental Strategies and Techniques, Eds. R. Wevers, N. Lutz, and J. V. Sweedler, Cambridge University Press P. Nemes, A. M. Knolhoff, S. S. Rubakhin, and J. V. Sweedler, Single-cell metabolomics: changes in the metabolome of freshly isolated and cultured neurons, ACS Chem. Neuro. 2012, 3, 782–792, DOI: 10.1021/cn300100u P. Nemes and A. Vertes, Ambient mass spectrometry for in vivo local analysis and in situ molecular tissue imaging, TrAC-Trends in Analytical Chemistry 2012, 34, 22–34, DOI: 10.1016/j.trac.2011.11.006 P. Nemes, H. Huang, and A. Vertes, Internal energy deposition and ion fragmentation in atmospheric-pressure mid-infrared laser ablation electrospray ionization, Phys. Chem. Chem. Phys. 2012, 14, 2501–2507, DOI: 10.1039/C2CP23411D S. S. Rubakhin, E. V. Romanova, P. Nemes, and J. V. Sweedler, Profiling metabolites and peptides in single cells, Nat. Methods 2011, 8, S20–S29, DOI: 10.1038/nmeth.1549 P. Nemes, A. M. Knolhoff, S. S. Rubakhin, and J. V. Sweedler, Metabolic differentiation of neuronal phenotypes by single-cell capillary electrophoresis electrospray ionization mass spectrometry, Anal. Chem. 2011, 83, 6810–6817, DOI: 10.1021/ac2015855 Highlighted by Analytical Chemistry as one of 30 articles on advances in modern metabolomics. P. Nemes and A. Vertes, Laser ablation electrospray ionization for atmospheric pressure molecular imaging mass spectrometry, Methods in Molecular Biology 2010, pp. 159–171, Eds. S. S. Rubakhin and J. V. Sweedler, Humana Press P. Nemes and A. Vertes, Atmospheric-pressure molecular imaging of biological tissues and biofilms by LAESI mass spectrometry, Journal of Visualized Experiments 2010, 43, DOI: 10.3791/2097 Available in video format at http://www.jove.com/details.php?id=2097. P. Nemes, A. S. Woods, and A. Vertes, Simultaneous small metabolite and lipid imaging in rat brain tissues and atmospheric pressure by laser ablation electrospray ionization mass spectrometry, Anal. Chem. 2010, 82, 982–988, DOI: 10.1021/ac902245p B. Shrestha, P. Nemes, and A. Vertes, Ablation and analysis of small cell populations and single cells by consecutive laser pulses, Appl. Phys. A 2010,DOI: 10.1007/s00339-010-5781-2 B. Shrestha, P. Nemes, J. Nazarian, Y. Hathout, E. P. Hoffman, and A. Vertes, Direct analysis of lipids and other metabolites in mouse brain tissue by AP IR-MALDI and reactive LAESI mass spectrometry, Analyst 2010, 135, 751–758, DOI: 10.1039/B922854C P. Nemes, A. A. Barton, and A. Vertes, Three-dimensional imaging of metabolites in tissues under native conditions by laser ablation electrospray ionization mass spectrometry,Anal. Chem. 2009, 81, 6668–6675, DOI: 10.1021/ac900745e P. Nemes, A. A. Barton, Y. Li, and A. Vertes, Ambient molecular imaging and depth profiling of live tissue by infrared laser ablation electrospray ionization mass spectrometry, Anal. Chem. 2008, 80, 4575–4582, DOI: 10.1021/ac8004082