个人简介

BS, Brigham Young University (1992) Ph.D., University of California, Berkeley (1997) Runyon-Winchell Postdoctoral Fellow, Harvard University (1998-2000) Awards: Karl G. Maeser Research and Creative Arts Award, Brigham Young University, 2014. Reed M. Izatt & James J. Christensen Faculty Excellence in Research Award, Brigham Young University, 2012. Brigham Young University, Young Scholar Award, 2008. Brigham Young University, College of Physical and Mathematical Sciences Young Scholar Award, 2008. American Chemical Society, Division of Analytical Chemistry Award for Young Investigators in Separation Science, 2007. Presidential Early Career Award for Scientists and Engineers (PECASE) – National Institutes of Health, 2006.

研究领域

Analytical Chemistry

Micro-and Nanometer-Scale Chemical Manipulation and Analysis: My group works at the interface between chemistry, engineering and biology. Thus, students receive broad technical training and are well poised to contribute in these key research fields. A common theme in my research is the interrelationship between biological molecules and miniaturization. We are utilizing miniaturization tools to detect and quantify clinically relevant biomolecules, and we are also applying DNA in forming nanoscale materials. A. Integrated microfluidic systems for preterm birth risk assessment. Preterm birth (PTB) is a serious issue, with approximately 10% of pregnancies resulting in a preterm delivery, frequently coupled with complications that lead to poor outcomes and increased medical costs. We are developing microfluidic systems that combine extraction, fluorescent labeling and separation all in a single microchip (Fig. 1). These devices will provide high-throughput, point of care screening from a finger stick quantity of blood to assess risk of a preterm delivery, weeks before contractions begin. B. Biotemplated nanofabrication of electronics: My group is leading an interdisciplinary team whose objective is to explore bottom-up methods for the fabrication of nanoscale electronic systems. We fold DNA into controlled nanoscale designs that can be converted into functional electronic elements after purification and metallization (Fig. 2). We are presently applying these methods in making metal-semiconductor junctions with linewidths as small as 5 nm. C. Low-cost microfluidic systems for rapid biomarker quantitation. We are developing simple, detectorless microfluidic systems for rapid analyte quantitation in resource-limited settings. These “flow valve” microdevices utilize capillary flow of solution and constrict in response to a target analyte (Fig. 3). The flow distance, which can be easily measured by simple visual inspection, can thus tell us the risk of a concentration of target molecule in the solution.

近期论文

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Pagaduan, J.P.; Ramsden, M.; O’Neill, K.; Woolley, A.T. Microchip Immunoaffinity Electrophoresis of Antibody-Thymidine Kinase 1 Complex. Electrophoresis in press (2015). Kumar, S.; Stout, J.M.; Hawkins, A.R.; Woolley, A.T. Ionic Strength Effects on Protein Trapping in Thin-Film Fabricated Nanochannels. In Micro Total Analysis Systems 2014, Jacobson, S.C and Kutter, J.P, Eds. Chemical and Biological Microsystems Society: San Diego, CA, 2014; pp 1344-1346. Chatterjee, D.; Yeakley, F.; Woolley, A.T. Flow-Valve Microfluidic Devices for Simple, Detectorless and Label-Free Quantitation of Nucleic Acids. In Micro Total Analysis Systems 2014, Jacobson, S.C and Kutter, J.P, Eds. Chemical and Biological Microsystems Society: San Diego, CA, 2014; pp 1021-1023. Chatterjee, D.; Mansfield, D.S.; Woolley, A.T. Microfluidic Devices for Label-Free and Non-Instrumented Quantitation of Unamplified Nucleic Acids by Flow Distance Measurement. Anal. Meth. 6, 8173-8179 (2014). Yang, R.; Pagaduan, J.V.; Yu, M.; Woolley, A.T. On Chip Preconcentration and Fluorescence Labeling of Model Proteins by Use of Monolithic Columns: Device Fabrication, Optimization, and Automation. Anal. Bioanal. Chem. in press (2014). Gates, E.P.; Dearden, A.M.; Woolley, A.T. DNA-templated Lithography and Nanofabrication for the Fabrication of Nanoscale Electronic Circuitry. Crit. Rev. Anal. Chem. 44, 354-370 (2014). Uprety, B.; Gates, E.P.; Geng, Y.; Woolley, A.T.; Harb, J.N. Site-specific Metallization of Multiple Metals on a Single DNA Origami Template. Langmuir 30, 1134-1141 (2014). Woolley, A.T.; Kumar, S.; Xuan, J.; Lee, M.L.; Tolley, H.D.; Hawkins, A.R. Size-based Protein Fractionation in Nanofluidic Channel Arrays. In Micro Total Analysis Systems 2013, Zengerle, R. Ed. Chemical and Biological Microsystems Society: San Diego, CA, 2013; pp 110-112. Rogers, C.I.; Oxborrow, J.B.; Anderson, R.R.; Tsai, L.-F.; Nordin, G.P.; Woolley, A.T. Microfluidic Valves Made From Polymerized Polyethylene Glycol Diacrylate. Sens. Actuators B 191, 438-444 (2014). Kumar, S.; Xuan, J.; Lee, M.L.; Tolley, H.D.; Hawkins, A.R.; Woolley, A.T. Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation.Lab Chip 13, 4591-4598 (2013). Liu, J.; Uprety, B.; Gyawali, S.; Woolley, A.T.; Myung, N.V.; Harb, J.N. Fabrication of DNA-Templated Te and Bi Te Nanowires by Galvanic Displacement. Langmuir 29 2 3 11176-11184 (2013). Gao, C.; Sun, X.; Woolley, A.T. Fluorescent Measurement of Affinity Binding Between Thrombin and its Aptamers using On-chip Affinity Monoliths.J. Chromatogr. A 1291, 92- 96 (2013). Geng, Y.; Pearson, A.C.; Gates, E.P.; Uprety, B.; Davis, R.C.; Harb, J.N.; Woolley, A.T. Electrically Conductive Gold and Copper Metallized DNA Origami Nanostructures. Langmuir 29, 3482-3490 (2013). Nge, P.N.; Rogers, C.I.; Woolley, A.T. Advances in Microfluidic Materials, Functions, Integration and Applications. Chem. Rev. 113, 2550-2583 (2013). Ness, S.J.; Anderson, R.R.; Hu, W.; Richards, D.C.; Oxborrow, J.; Gustafson, T.; Tsai, B.; Kim, S.; Mazzeo, B.; Woolley, A.; Nordin, G.P. Weak Adsorption-Induced Surface Stress for Streptavidin Binding to Biotin Tethered to Silicon Microcantilever Arrays. IEEE Sensors 13, 959-968 (2013). Nge, P.N.; Pagaduan, J.V.; Yu, M.; Woolley, A.T. Microfluidic Chips with Reversed- Phase Monoliths for Solid Phase Extraction and On-Chip Labeling.J. Chromatogr. A 1261, 129-135 (2012). Chatterjee, D.; Mansfield, D.S.; Anderson, N.G.; Subedi, S.; Woolley, A.T. “Flow Valve” Microfluidic Devices for Simple, Detectorless and Label-Free Analyte Quantitation.Anal. Chem. 84, 7057-7063 (2012). Pearson, A.C.; Liu, J.; Pound, E.; Uprety, B.; Woolley, A.T.; Davis, R.C.; Harb, J.N. DNA Origami Metallized Site Specifically to Form Electrically Conductive Nanowires. J. Phys. Chem. B 116, 10551-10560 (2012). Ness, S.J.; Kim, S.; Woolley, A.T.; Nordin, G.P. Single-Sided Inkjet Functionalization of Silicon Photonic Microcantilevers. Sens. Actuat. B 161, 80-87 (2012). Xuan, J.; Hamblin, M.N.; Stout, J.M.; Tolley, H.D.; Maynes, D.R.; Woolley, A.T.; Hawkins, A.R.; Lee, M.L. Surfactant Addition and Alternating Current Electrophoretic Oscillation During Size Fractionation of Nanoparticles in Channels with Two or Three Different Height Segments. J. Chromatogr. A 1218, 9102-9110 (2011).