Kobryn, Kerri - University of Saskatchewan - Department of Microbiology and Immunology

个人简介

BSc. (Hon) (1992) Genetics, University of Western Ontario, Canada PhD (1997) Genetics, University of Glasgow, UK Biochemistry and Molecular Biological studies of the Lyme Disease spirochete Borrelia burgdorferi.

研究领域

Lyme disease is an important emerging infection in Canada transmitted by ticks infected with Borrelia burgdorferi. The Lyme disease spirochetes are unique among human pathogens in having a linear chromosome and plasmids terminated by unusual hairpin telomeres. The lab studies the enzyme, ResT, which forms these hairpin telomeres with a view towards eventual rational design of Borrelia-specific drugs. ResT is related to proteins that in other bacterial species control chromosome segregation. Projects on the biochemistry of this enzyme are available as well as projects to identify and characterize accessory factors required for telomere formation and maintenance. Also available is a project to investigate the origins of genome linearity by recapitulating chromosome linearization in E. coli by introduction of ResT and its substrate into E. coli and rational mutagenesis of appropriate endogenous E. coliproteins.

近期论文

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Huang, SH, Cozart, M.R., Hart, M.A., Kobryn, K. The Borrelia burgdorferi telomere resolvase, ResT, possesses ATP-dependent DNA unwinding activity. Nucl. Acids Res. 2016. doi: 10.1093/nar/gkw1243 Huang SH and Kobryn K. The Borrelia burgdorferi telomere resolvase, ResT, anneals ssDNA complexed with its cognate ssDNA-binding protein. Nucl. Acids Res. 2016. doi: 10.1093/nar/gkw344 Lucyshyn D, Huang SH, Kobryn K. Spring loading a pre-cleavage intermediate for hairpin telomere formation. Nucl. Acids Res. 2015. doi:10.1093/nar/gkv497 Mir T, Huang SH, Kobryn K. The telomere resolvase of the Lyme disease spirochete, Borrelia burgdorferi, promotes DNA single-strand annealing and strand exchange. Nucleic Acids Res. 2013 Sep 17. [Epub ahead of print] doi: 10.1093/nar/gkt832 Briffotaux J, Kobryn K. Preventing broken Borrelia telomeres: ResT couples dual hairpin telomere formation with product release. J Biol Chem. 2010 Dec 24;285(52):41010-8. Epub 2010 Oct 14. Chaconas G, Kobryn K. Structure, function, and evolution of linear replicons in Borrelia. Annu Rev Microbiol. 2010;64:185-202. Review. Kobryn K, Briffotaux J, Karpov V. Holliday junction formation by the Borrelia burgdorferi telomere resolvase, ResT: implications for the origin of genome linearity. Mol Microbiol. 2009 Mar;71(5):1117-30. Bankhead T, Kobryn K, Chaconas G. Unexpected twist: harnessing the energy in positive supercoils to control telomere resolution. Mol Microbiol. 2006 Nov;62(3):895-905 Kobryn K, Burgin AB, Chaconas G. Uncoupling the chemical steps of telomere resolution by ResT. J Biol Chem; 2005 Jul 22;280(29):26788-95.