研究领域

Cellular origins of plant shape Microtubules, cell walls and cell shape How patterns of cell division, expansion, and differentiation influence the growth and morphology of tissues, organs, and organism

The cellular origins of plant shape Plant shape is defined by controlling where, when, and how cells divide and expand. Our group is interested in how patterns of cell division, expansion, and differentiation influence the growth and morphology of tissues, organs and organism. The main subcellular components governing cellular differentiation include the microtubule and actin cytoskeletons, the cell wall, and membrane/endomembrane system. Using the model plant Arabidopsis thaliana, we use molecular-genetics and live-cell imaging of green fluorescent protein variants to dissect these elements in terms of their role in cell development. Microtubules, cell walls and cell shape Control of cell division and differentiation relies heavily on the microtubule cytoskeleton; a complex network of tubulin polymers that dynamically arrange into a variety of three-dimensional configurations. As in all eukaryotic cells, microtubules in plants also function to define cellular polarity and shape, separate chromosomes, drive cytokinesis, and carry a variety of organelles and molecules around the cell. With respect to cell wall function, two plant-specific microtubule assemblies—the cytokinetic phragmoplast and the interphase cortical array—are paramount. The phragmoplast drives cell plate formation and expansion during cytokinesis, and the cortical array lines the cell cortex in varied microtubule patterns (parallel, net-like, and large bundles), where it influences the orientation and structure of cellulose microfibrils within the surrounding cell wall, thereby defining how cells expand in response to cellular turgor pressure.

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Katherine Celler, Miki Fujita, Eiko Kawamura, Chris Ambrose, Klaus Herburger, Andreas Holzinger, and Geoffrey O. Wasteneys. "Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy and Live Cell Imaging.." In Cytoskeleton Methods and Protocols, 3rd, edited by Gavin, Ray H, 155-184. New York: Springer, 2015. Ambrose, J, Geoffrey Wasteneys. "Microtubule initiation from the nuclear surface controls cortical microtubule growth polarity and orientation in Arabidopsis thaliana." Plant Cell and Physiology55, 9 (2014): 1636-1645. Ambrose, J, Yuan Ruan, John Gardiner, Laura Tamblyn, Amanda Catching, Viktor Kirik, Jan Marc, Robyn Overall, Geoffrey O. Wasteneys. "CLASP Interacts with Sorting Nexin 1 to Link Microtubules and Auxin Transport via PIN2 Recycling in Arabidopsis thaliana." Develpomental Cell24, 6 (2013): 649-659. Ambrose JC*, Allan DeBono*, Geoffrey O. Wasteneys . "Cell geometry guides the dynamic targeting of apoplastic GPI-linked Lipid Transfer Protein to cell wall elements and cell borders in Arabidopsis thaliana." PLOS One8, 11 (2013): e81215-*Authors contributed equally. Ambrose, J, Allard JF, Cytrynbaum EN, Wasteneys GO. "A cell-edge-barrier mechanism drives cell-wide cortical microtubule orientation in Arabidopsis." Nature Communications2, 430 (2011): 430. Ambrose, J, Wasteneys, GO. "Cell edges accumulate gamma tubulin complex components and nucleate microtubules following cytokinesis in Arabidopsis thaliana cells." PLOS One6, 11 (2011). Ambrose, J. "Nanoscale and geometric influences on the microtubule cytoskeleton in plants; thinking inside and outside the box." Protoplasma249 (2011). Allard JF, Ambrose JC, Wasteneys GO, Cytrynbaum EN. "A mechanochemical model explains interactions between cortical microtubules in plants." Biophysical Journal4 (2010): 1082-1090. Wasteneys, GO, Ambrose, JC. "Spatial organization of plant cortical microtubules: close encounters of the 2D kind." Trends in Cell Biology19 (2009): 62-71. Ambrose, Chris, Cyr, Richard. "Mitotic Spindle Organization by the Preprophase Band.." Mol Plant1 (2008): 950-960. Ambrose, C, Wasteneys, GO. "CLASP modulates microtubule-cortex interaction during self-organization of acentrosomal microtubules." Mol Biol Cell19 (2008): 4730-4737. Ambrose, J, Cyr, RJ. "Mitotic Spindle Assembly and Function." In Cell Division Control in Plants, edited by D.P.S. Verma and Z. Hong, 141-167. Berlin Heidelberg: Springer-Verlag, 2007. Ambrose, J.C., Li, W., Marcus, A., Ma, H., and Cyr, R. "A minus-end-directed kinesin with plus-end tracking protein activity is involved in spindle morphogenesis.." Mol Biol Cell16 (2005): 1584-1592. Marcus, A.I., Ambrose, J.C., Blickley, L., Hancock, W.O., and Cyr, R.J. . "Arabidopsis thaliana protein, ATK1, is a minus-end directed kinesin that exhibits non-processive movement.." Cell Motil Cytoskeleton52 (2002): 144-150.