个人简介

Vanderbilt University Medical Center, NIH Postdoctoral Fellow, 2006-2008 University of Virginia, Ph.D., 2006 Mississippi State University, B.S., 2002

研究领域

Analytical

Bioanalytical Chemistry: Novel microanalytical tools for studying fundamentals of glucose homeostasis and diabetes. The Easley laboratory is focused on the development of novel microanalytical techniques that allow us to perform unique experiments on biological systems. One major focus of our laboratory involves the development of microfluidic methods to study secretions from small numbers of cells in the form of intact, primary tissue. We are interested in the consequences of cellular architecture in paracrine signaling within pancreatic islets of Langerhans, the functional units of insulin secretion that help to maintain blood glucose homeostasis. Since it is now understood that adipose tissue (fat) is an active endocrine organ, we are also using microfluidics to help measure rapid secretion from primary adipocytes (fat cells). These methods should help improve our understanding of hormone secretion from adipose tissue. Debilitating conditions such as diabetes, obesity, and metabolic syndrome are fundamentally linked to these types of tissue. The second major focus in our lab is to utilize DNA-antibody conjugates and DNA aptamers in cooperative sensing approaches, allowing picomolar detection limits of protein and small molecule analytes from small volumes of sample. Concurrently, we are developing cooperative methods to select DNA aptamers for high affinity and specificity in target binding. Research in our laboratory spans several scientific disciplines, from fundamental analytical chemistry to molecular and cellular biology, conducted through combinations of novel and traditional techniques: microfluidics, fluorescence microscopy and spectroscopy, passive flow control, molecular biology, aptamer selection, and electrophoresis. Please visit our RESEARCH WEB PAGE for more details.

近期论文

查看导师最新文章 （温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Leah A. Godwin, Jessica C. Brooks, Lauren D. Hoepfner, Desiree Wanders, Robert L. Judd, and Christopher J. Easley*, A Microfluidic Interface Design for the Culture and Sampling of Adiponectin from Primary Adipocytes, Analyst 2015, 140, 1019-1025. PDF Maynard, B. A.; Brooks, J. C.; Hardy, E. E.; Easley, C. J.; Gorden, A. E. V., "Synthesis, structural characterization, electronic spectroscopy, and microfluidic detection of Cu+2 and UO2+2 [di-tert-butyl-salphenazine] complexes," Dalton Trans. 2015, DOI: 10.1039/C5DT00106D. PDF Jiaming Hu, Yajiao Yu, Jessica C. Brooks, Leah A. Godwin, Subramaniam Somasundaram, Ferdous Torabinejad, Joonyul Kim, Curtis Shannon*, and Christopher J. Easley*, A Reusable Electrochemical Proximity Assay for Highly Selective, Real-Time Protein Quantitation in Biological Matrices, J. Am. Chem. Soc. 2014, 136, 8467-8474. PDF Cheryl J. DeJournette, Joonyul Kim, Haley Medlen, Xiangpeng Li, Luke J. Vincent, and Christopher J. Easley, Creating Biocompatible Oil-Water Interfaces without Synthesis: Direct Interactions between Primary Amines and Carboxylated Perfluorocarbon Surfactants, Anal. Chem. 2013, 85, 10556-10564. PDF Leah A. Godwin, Kennon S. Deal, Lauren D. Hoepfner, Louis A. Jackson, Christopher J. Easley, Measurement of Microchannel Fluidic Resistance with a Standard Voltage Meter, Anal. Chim. Acta 2013, 758, 101-107. PDF Jiaming Hu, Tanyu Wang, Joonyul Kim, Curtis Shannon*, Christopher J. Easley*, Quantitation of femtomolar protein levels via direct readout with the electrochemical proximity assay, J. Am. Chem. Soc. 2012, 134, 7066–7072. PDF Daniel W. Horn; K. P. Tracy; Christopher J. Easley, Virginia A. Davis, Lysozyme Dispersed Single-Walled Carbon Nanotubes: Interaction and Activity, J. Phys. Chem. C 2012, 116, 10341–10348. PDF Kennon S. Deal and Christopher J. Easley, A Self-Regulated, Droplet-Based Sample Chopper for Microfluidic Absorbance Detection, Analytical Chemistry 2012, 84, 1510–1516. PDF Leah A. Godwin, Meagan E. Pilkerton, Kennon S. Deal, Desiree Wanders, Robert L. Judd, and Christopher J. Easley, A passively operated microfluidic device for stimulation and secretion sampling of single pancreatic islets, Analytical Chemistry, 83 (2011) 7166–7172. PDF Jiaming Hu and Christopher J. Easley, A Simple and Rapid Approach for Measurement of Dissociation Constants of DNA Aptamers against Proteins and Small Molecules via Automated Microchip Electrophoresis, Analyst, 136 (2011) 3461-3468. PDF Joonyul Kim and Christopher J. Easley, Isothermal DNA Amplification in Bioanalysis: Strategies and Applications, Bioanalysis, 3 (2011) 227-239. Author's PDF, Publisher's PDF Joonyul Kim, Jiaming Hu, Rebecca S. Sollie, Christopher J. Easley, Improvement of sensitivity and dynamic range in proximity ligation assays by asymmetric connector hybridization, Analytical Chemistry, 82 (2010) 6976-6982. PDF Christopher J. Easley, Jonathan V. Rocheleau, W. Steven Head, and David W. Piston, Quantitative measurement of zinc secretion from pancreatic islets with high temporal resolution using droplet-based microfluidics, Analytical Chemistry, 81 (2009) 9086-9095. PDF Daniel C. Leslie, Christopher J. Easley, Erkin Seker, James M. Karlinsey, Marcel Utz, Matthew R. Begley, and James P. Landers, Frequency-specific flow control in microfluidic circuits with passive elastomeric features, Nature Physics, 5 (2009) 231-235. PDF Christopher J. Easley, Richard K. P. Benninger, Jesse H. Shaver, W. Steven Head, and David W. Piston, Rapid and inexpensive fabrication of polymeric microfluidic devices via toner transfer masking, Lab on a Chip, 9 (2009) 1119-1127. PDF Shu Mao, Richard K. P. Benninger, Yuling Yan, Chutima Petchprayoon, David Jackson, Christopher J. Easley, David W. Piston, and Gerard Marriott, Optical lock-in detection of fluorescence resonance energy transfer using synthetic and genetically-encoded optical switches, Biophysical Journal, 94 (2008) 4515-4524. PDF Christopher J. Easley, Joseph A. C. Humphrey, and James P. Landers, Thermal isolation of microchip reaction chambers for rapid non-contact DNA amplification, Journal of Micromechanics and Microengineering, 17 (2007) 1758-1766. PDF Ki-Ho Han, Rachel D. McConnell, Christopher J. Easley, Joan M. Bienvenue, Jerome P. Ferrance, James P. Landers, and A. Bruno Frazier, An active microfluidic system packaging technology, Sensors and Actuators B: Chemical, 122 (2007) 337-346. PDF Christopher J. Easley, James M. Karlinsey, Joan M. Bienvenue, Lindsay A. Legendre, Michael G. Roper, Sanford H. Feldman, Molly A. Hughes, Erik L. Hewlett, Tod J. Merkel, Jerome P. Ferrance, and James P. Landers, A fully-integrated microfluidic genetic analysis system with sample in-answer out capability, Proceedings of the National Academy of Sciences USA, 103 (2006) 19272-19277. PDF [Highlighted in Science as an Editor's Choice, 19 January: 315 (2007) 5810. PDF ; also in Nature Biotechnology as a Research Highlight, January: 25 (2007) 69; and in Analytical Chemistry as a Bio Sphere news article, February: 79 (2007) 809.] Michael G. Roper, Christopher J. Easley, Lindsay A. Legendre, Joseph A. C. Humphrey, and James P. Landers, Infrared temperature control system for a completely noncontact polymerase chain reaction in microfluidic chips, Analytical Chemistry, 79 (2007) 1294-1300. PDF