个人简介

Emory University School of Medicine, Ph.D., Biochemistry and Molecular Biology, 1998 Georgia Institute of Technology, B.S., Chemistry, 1991

研究领域

Analytical Chemistry/Inorganic Chemistry/Organic Chemistry/Physical Chemistry/Biological Chemistry/Chemical Education/Computational/Theoretical Chemistry/Environmental/Atmospheric Chemistry/Materials Chemistry and Nanoscience

Our research is aimed at increasing our understanding of how increased functional diversity leads to finer control of biological processes. The hope is that by understanding the role of PTMs, we will not only more accurately describe fundamental biological processes but will also elucidate novel therapeutic targets in disease states such as type II diabetes, congenital muscular dystophy and cancer in which these processes have become dysregulated.

近期论文

查看导师最新文章 （温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

JL Praissman, L. Wells. 2014. Mammalian o-mannosylation pathway: glycan structures, enzymes, and protein substrates. Biochemistry 53(19): 3066-3078. PMID:24786756 L Zhang, ZA Syed, I van Dijk Hard, JM Lim, L Wells, KG Ten Hagen. 2014. O-Glycosylation regulates polarized secretion by modulating Tango1 stability. Proc Natl Acad Sci USA 111(20): 7296-7301. PMID:24799692 WS York, S. Agravat, KF Aoki-Kinoshita, R. McBride, MP Campbell, CE Costello, A. Dell, T Feizi, SM Haslam, N.G. Karlsson, KH Khoo, D. Kolarich, Y. Liu, M.V. Novotny, NH Packer, JC Paulson, E. Rapp, R Razinger, PM Rudd, DF Smith, WB Struwe, M. Tiemeyer, L. Wells, J. Zaia, C Kettner. 2014. MIRAGE: the minimum information required for a glycomics experiment. Glycobiology 24(5): 402-406. PMID:24653214 K Vaidyanathan, S Durning, L Wells. 2014. Functional O-GlcNAc modifications: implications in molecular regulation and pathophysiology. Crit Rev Biochem Mol Biol Mar-Apr 49(2): 140-163. PMID:24524620 VM Panin, L Wells. 2014. Protein O-mannosylation in metazoan organisms. Curr Protoc Protein Sci Feb. 3; 75: Unit 12.12: doi: 10.1002/047-ps1212s75. PMID:24510673 M Porterfield, P Zhao, H Han, J Cunningham, K Aoki, DD Von Hoff, MJ Demeure, JM Pierce, M Tiemeyer, L Wells. 2014. Discrimination between Adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis. J Proteome Res Feb 7; 13(2): 395-407. PMID:24328148 JL Praissman, DH Live, S Wang, A. Ramiah, ZS Chinoy, GJ Boons, KW Moremen, L Wells. 2014. B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of alpha-dystroglycan. . eLife Oct 3;3: doi:10.7554/-eLife.03943. PMID:25279697 JL Praissman, DH Live, S. Wang, A. Ramiah, ZS Chinoy, GJ Boons, KW Moremen, L. Wells. 2014. B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of alpha-dystroglycan.. eLife Oct 3;3: doi:10.7554/-eLife.03943. PMID:25279697 L. Wells, G.W. Hart. 2013. Glycomics: Building upon proteomics to advance glycosciences. Mol. Cell Proteomics 12: 833-835. PMID:23378519 J. Kang, Z. Shen, J.M. Lim, H. Handa, L. Wells, D. Tantin. 2013. Regulation of Oct1/Pou2f1 transcription activity by O-GlcNAcylation. FASEB J. 7: 2807-2817. PMID:23580612 L. Wells. 2013. The O-mannosylation pathway: Glycosyltransferases and proteins implicated in congenital muscular dystrophy. J. Biol. Chem. 288: 6930-6395. PMID:23329833 P. Zhao, S.H. Stalnaker, L. Wells. 2013. Approaches for site mapping and quantification of O-linked glycopeptides. Methods Mol. Biol. 951: 229-244. PMID:23296534 S. Liu, H. Im, A. Bairoch, M. Cristofanilli, R. Chen, E.W. Deutsch, S. Dalton, D. Fenyo, S. Fanayan, C. Gates, P. Gaudet, M. Hincapie, S. Hanash, H. Kim, S.K. Jeong, E. Lundberg, G. Mias, R. Menon, Z. Mu, E. Nice, Y.K. Paik, M. Uhlen, L. Wells, S.L. Wu, F. Yan, F. Zhang, Y. Zhang, M. Snyder, G.S. Omenn, R.C. Beavis, W.S. Hancock. 2013. A chromosome-centric human proteome project (C-HPP) to characterize the sets of proteins encoded in chromosome 17. J. Proteome Res. 12: 45-57. PMID:23259914 D. Live, L. Wells, G.J. Boons. 2013. Dissecting the molecular basis for the role of O-mannosylation pathway in disease: alpha-dystroglycan and forms of muscular dystrophy. ChemBioChem Dec 16; 14(18): 2392-2402. PMID:24318691 L. Meng, F. Forouhar, D. Thieker, Z. Gao, A. Ramiah, H.A. Moniz, J. Seetharaman, S. Milaninia, M. Su, R. Bridger, L. Veillon, P. Azadi, G. Kornhaber, L. Wells, G.T. Montelione, R.J. Woods, L. Tong, K.W. Moremen. 2013. Enzymatic basis for N-glycan sialylation: Structure of mammalian alpha2,6-sialytransferase (ST6GAL1) reveals conserved and unique features for glycan sialytion. J. Biol. Chem. 288(48): 34680-34698. PMID:24155237 M. Porterfield, Z Peng, H Haiyong, J Cunningham, K. Aoki, D Von, J Demeure, M. Pierce, M. Tiemeyer, L. Wells. 2013. Discrimination between Adenocarcinoma and Normal Pancreatic Ductal Fluid by Proteomic and Glycomic Analysis. J. Proteome Res. In press. L Meng, F Forouhar, D Thieker, Z Gao, A Ramiah, H Moniz, Y Xiang, J Seetharaman, S Milianinia, M Su, R Bridger, L Veillon, P Azadi, G Kornhaber, L Wells, GT Montelione, RJ Woods, L Tong, KW Moremen. 2013. Enzymatic Basis for N-Glycan Sialylation: STRUCTURE OF RAT alpha2,6-SIALYLTRANSFERASE (ST6GAL1) REVEALS CONSERVED AND UNIQUE FEATURES FOR GLYCAN SIALYATION. J. Biol. Chem. 288(48): 34680-34698. PMID:24155237 P. Zhao, A.V. Nairn, S. Hester, K.W. Moremen, R.M. ORegan, G. Oprea, L. Wells, M. Pierce, K.L. Abbott. 2012. Proteomic identification of glycosylphosphatidylinositol anchor-dependent membrane proteins elevated in breat carcinoma. J.Biol Chem. 287: 25230-25240. PMID:22654114 A.V. Nairn, K. Aoki, M. Dela, M. Porterfield, J.M. Lim, M. Kulik, J.M. Pierce, L. Wells, S. Dalton, M. Tiemeyer, K.W. Moremen. 2012. Regulation of glycan structures in murine embryonic stem cells: Combined transcript profiling of glycan-related genes and glycan structural analysis. J. Biol. Chem. 287: 37835-37856. PMID:22988249 J.K. Lee, R.T. Matthews, J.M. Lim, K. Swanier, L. Wells, J.M. Pierce. 2012. Developmental expression of the neuron-specific N-caetylglucosaminyltransferase Vb (GnT-Vb/lX) and identification of its in vivo glycan products in comparison with those of its paralog, GnT-V . J.Biol. Chem. 287: 28526-28536. PMID:22715095 J.J. Fong, B.L. Nguyen, R. Bridger, E.E. Medrano, L. Wells, S. Pan, R.N. Sifers. 2012. O-GlcNAc is a novel regulator of mitosis-specific phosphorylations on histone H3. J.Biol. Chem. 287: 12195-12203. PMID:22371497 D.T. Tran, J.M. Lim, M. Liu, S.H. Stainaker, L. Wells, K.G. Ten Hagen, D. Live. 2012. Glycosylation of alpha-dystroglycan: O-mannosylation influences the subsequent addition of GalNAc by ppGalNAcTs. J. Biol. Chem. 287: 20967-20974. PMID:22549772 S.J. Polizzi, R.M. Walsh, Jr., W.B. Peeples, J.M. Lim, L. Wells, Z.A. Wood. 2012. Human UDP-α-D-xylose synthase and Escherichia coli ArnA conserve a conformational shunt that controls whether xylose or 4-keto-xylose is produced. Biochemistry 51: 8844-8855. PMID:23072385 P. Zhao, R. Viner, C.F. Teo, G.J. Boons, D. Horn, L. Wells. 2011. Combining high-energy C-trap dissociation and electron transfer dissociation for protein O-GlcNAc modification site assignment. J. Proteome Res. 10: 4088-4104. K. Mo, T. Fang, S. Stainaker, P.S. Kirby, M. Liu, L. Wells, M. Pierce, D.H. Live, G.J. Boons. 2011. Synthetic, structural and biosynthetic studies of an unusual phosphoglycopeptide derived from α-dystroglycan. J. Am. Chem. Soc. 133: 14418-14430. S.H. Stainaker, R. Stuart, L. Wells. 2011. Mammalian O-mannosylation: unvolved questions of structure/function. Curr. Opin. Struct. Biol. 21: 603-609. PMID:21945038 J. Barnes, J.M Lim, A. Godard, F. Blanchard, L. Wells, R. Steet. 2011. Extensive mannose phosphorylation on leugemic inhibitory factor (LIF)controls its extracellular levels by multiple mechanisms. J. Biol. Chem. 286: 24855-24864. PMID:21613225 S.H. Stainaker, K. Aoki, J.M. Lim, M. Porterfield, M. Liu, J.S. Satz, S. Buskirk, Y. Xiong, P. Zhang, K.P. Campbell, H. Hu, D. Live, M. Tiemeyer, L. Wells. 2011. Glycomic analyses of mouse models of congenital muscular dystrophy. J. Biol. Chem. 286: 21180-21190. PMID:21460210 C.F. Teo, E.E. Wollaston-Hayden, L. Wells. 2010. Hexosamine flux, the O-GlcNAc modification, and the development of insulin resistance in adipocytes. Mol. Cell. Endocrinol. 318: 44-53. PMID:19799964 S. Brimble, E.E. Wollaston-Hayden, C.F. Teo, A.C. Morris, L. Wells. 2010. The role of the O-GlcNAc modification in regulating eukaryotic gene expression. Curr. Signal Transduc. Ther. 5: 12-24. T. Yoshida-Moriguchi, S. Davis, S. Hammond, L. Yu, S. Kunz, M. Madson, M.B.A. Oldstone, H. Schacter, L. Wells, K.P. Campbell. 2010. O-mannosyl phosphorylation of slpha-dystroglycan: A novel modification required for laminin-binding. Science 327: 88-92. PMID:20044576 M.A. Humbard, H.V. Miranda, J.M. Lim, D.J. Krause, J.R. Pritz, G.K. Zhou, S. Chen, L. Wells, J.A. Maupin-Farlow. 2010. Ubiquitin-like Small Archaeal Modifier Proteins (SAMPs) in Haloferaz volcanii. Nature 463: 54-60. PMID:20054389 N. Nakamura, S.H. Stainaker, D. Lyalin, O. Lavrova, L. Wells, V.M. Panin. 2010. Drosophila dystroglycan is a target of O-mannosyltransferase activity of two protein O-mannosyltransferases, Rotated Abdomen and Twisted. Glycobiology 20: 381-394. PMID:19969597 C.F. Teo, S. Ingale, G.A. Elsayed, M.A. Wolfert, L. Wells, G.J. Boons. 2010. Generation of O-GlcNAc specific monoclonal antibodies using a novel synthetic immunogen. Nature Chem. Biol. 6: 338-343. PMID:20305658 S.H. Stalnaker, S. Hashmi, J-M Lim, K. Aoki, M. Porterfield, G. Gutierrez-Sanchez, J. Wheeler, J.M. Ervasti, C. Bergmann, M. Tiemeyer, L. Wells. 2010. Site-mapping and characterization of O-glycan structures on alpha-dystroglycan isolated from rabbit skeletal muscle. J. Biol. Chem. 285: 24882-24891. PMID:20507986 S.H. Stalnaker, S. Hashmi, J-M Lim, K. Aoki, M. Porterfield, G. Gutierrez-Sanchez, J. Wheeler, J.M. Ervasti, C. Bergmann, M. Tiemeyer, L. Wells. 2010. Site-mapping and characterization of O-glycan structures on alpha-dystroglycan isolated from rabbit skeletal muscle. J. Biol. Chem. 285: 24882-24891. PMID:20507986 K.L. Abbott, J. Lim, L. Wells, B.B. Benigno, J.F. McDonald, M. Pierce. 2010. Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics 10: 470-481. PMID:19953551 A.D. Harper, S.H. Stalnaker, L. Wells, A. Darvill, R. Thornburg, W.S. York. 2010. Interaction of Nectarin 4 with a fungal protein triggers a microbial surveillance and defense mechanism in nectar. Phytochemistry 71: 1963-1969. PMID:20970816 R. Orlando, J.M. Lim, J.A. Atwood, P.M. Angel, M. Feng, G. Alvarez-Manilla, K.W. Moremen, W.S. York, M. Tiemeyer, J.M. Pierce, S. Dalton, R.L. Wells. 2009. IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J. Proteome Res. 8: 3816-3823. PMID:19449840