Chuong, Simon D.X. - University of Waterloo

个人简介

Dr. Chuong carries out research in plant cell and molecular biology. His research program aims to investigate factors that are involved in the development of single-cell C4 photosynthesis in several species of land plants. By understanding how these fascinating single-cell systems work, he hopes to provide researchers with the tools to improve yields, carbon fixation and stress tolerance of everyday crops. Plant Cell and Molecular Biology Plant Physiology C4 Photosynthesis Light and Electron Microscopy Histological and Immunohistological techniques Laurier’s Graduate Faculty; Department of Biology, Wilfrid Laurier University Journal Editorship Horticulture, Environment and Biotechnology Frontiers in Biology Professional Associations Canadian Botanical Association Canadian Botanical Association, Plant Development Section Chair Canadian Society of Plant Biologists University of Waterloo Service Undergraduate Advisor (Honours Biology, China 2+2) Faculty of Science Committee Representative Department of Biology Safety Coordinator 2003 PhD Plant Cellular and Molecular Biology, University of Calgary 1998 MSc Developmental Plant Biology, University of Calgary 1992 BSc Botany, University of Calgary

研究领域

Research in the Chuong’s lab focuses on understanding the cellular and molecular mechanisms that regulate the structure and function of single-cell C4 photosynthesis in two genera of terrestrial plants. In particular, his research group investigates factors that are involved in the movement of organelles and localization of macromolecules leading to the establishment of anatomical and biochemical polarity within two cytoplasmic regions of the cell. Dr. Chuong’s research uses a multifaceted approach, combining biochemistry, molecular biology, physiology, and cell biology techniques, from standard immunofluorescence and high resolution immunoelectron microscopy to the latest imaging techniques involving fluorescently-tagged proteins in living cells to address these research interests. The results will provide crucial information on the development and evolution of single-cell C4 photosynthesis in terrestrial plants. Understanding the underlying regulatory mechanisms responsible for the development of the intracellular compartmentalization of organelles and biochemistry in these fascinating single-cell C4 systems is important as it can provide opportunities for introducing their unique characteristics into C3 crop species to increase carbon fixation and yield under unfavourable conditions such as drought, high temperatures, and salinity where CO2 availability becomes limiting.

近期论文

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Northmore JA, Rustum A, Sigurdson D, Schoor S and Chuong SDX. 2016. Thidiazuron induces high-frequency indirect shoot organogenesis of Bienertia sinusperisici: a single-cell C4 species. Plant Cell Tiss Organ Cult.126:141-151. Lung SC, Sigurdson D, Schoor S, Yanagisawa M, Yeung K, Liu MQ and Chuong SDX. 2015. Protoplast isolation and staining. In: Plant Microtechniques and Protocols. Eds. Yeung ECT, Stasolla C, Sumner MJ, Huang BQ. Springer International Publishing. pp. 197-211. Lung SC, Smith MD, Gwynne W, Weston, K, Secord N and Chuong SDX. 2014. The C-terminus of Bienertia sinuspersici Toc159 receptor contains essential motifs for its targeting and anchorage to the chloroplast outer membrane. Front Plant Sci 5:722. Lung SC and Chuong SDX. 2012. A transit peptide-like sorting signal at the C-terminus directs the Bienertia sinuspersici preprotein receptor Toc159 to the chloroplast outer membrane. Plant Cell 24:1560-1578. Lung SC, Yanagisawa M and Chuong SDX. 2012. Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici. Plant Methods 8:8 Lung SC, Yanagisawa M and Chuong SDX. 2011. Protoplast isolation and transient gene expression in the single-cell C4 species, Bienertia sinuspersici. Plant Cell Rep. 30:473-484. Chuong SDX, Franceschi VR and Edwards GE. 2006. The cytoskeleton maintains organelle partitioning required for single cell C4 photosynthesis in Chenopodiaceae species. Plant Cell 18:2207-2223.