Rothstein, Steven - University of Guelph - Department of Molecular & Cellular Biology

个人简介

BA - Swarthmore College PhD- University of Wisconsin

研究领域

My research interest is to use molecular genetic, biochemical and genomic information to understand problems of fundamental importance in plant biology which also have an impact on the improvement of important crop plants. One of the most important issues in crop agriculture is the economic and environmental cost of using large quantities of nitrogen fertilizer. Our current principle interest is in understanding the regulation of nitrogen metabolism with the long-term goal of generating the basic knowledge necessary to be able to improve the nitrogen use efficiency of crop plants. We are utilizing the genetic and genomic resources developed by the Arabidopsis scientific community to study this problem. In particular, we are interested in identifying regulatory genes important for these processes and manipulating their expression so that we can learn how to alter this trait in a positive fashion. The genes to be tested are being identified using a combination of bioinformatics analysis of sequence information, transcript profiling, biochemical analysis and by studying a large number of defined mutations in the genes of interest.

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

D Yan, V Easwaran, V Chau, M Okamoto, M Ierullo, M Kimura, A Endo, R Yano, A Pasha, Y Gong, Y-M Bi, N Provard, D Guttman, A Krapp, SJ Rothstein and E Nambara. NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis. Nature Communications doi: 10.1038/ncomm13179, 2016. K Mahmood, Z Xu, A El-Kereamy, JA Casaretto and SJ Rothstein. The Arabidopsis Transcription Factor ANAC032 Represses Anthocyanin biosynthesis in Response to High Sucrose and Oxidative and Abiotic Stresses. Frontiers in Plant Science doi: 10.3389/fpls.2016.01548, 2016. K Mahmood, A El-Dereamy, S-H Kim, E Nambara and SJ Rothstein. ANAC032 Positively Regulates Age-Dependent and Stress-Induced Senescence in Arabidopsis thaliana. Plant and Cell Physiology 57 2029-2046, 2016. K Ranathunge, L Schreiber, Y-M Bi and SJ Rothstein. Ammonium-induces architectural and anatomic changes with altered suberin and lignin levels significantly change water and solute permeabilities of rice (Oryza sativa L.) roots. Planta 243 231-249, 2016. L Gaufichon, SJ Rothstein and A Suzuki. Asparagine metabolic pathways in Arabidopsis. Plant and Cell Physiology 19 5785-5798, 2016. JA Casaretto, A El-Kereamy, B Zeng, SM Stiegelmeyer, X Chen, Y-M Bi, and SJ Rothstein. Expression of OsMYB55 in maize activates stress-responsive genes and enhances heat and drought tolerance. BMC Genomics 17 doi: 10.1185/s12864-016-2659-5, 2015. A El-Kereamy, Y-M Bi, K. Mahmood, K. Ranathunge, MW Yaish and SJ Rothstein. Overexpression of the CC-type glutoredoxin, OsGRX6 affects hormone and nitrogen status in rice plants. Frontiers in Plant Science doi: 10.3389/fps.2015.00934, 2015. G Lu, V Coneva, JA Cassaretto, S Ying, K Mahmood, F Liu, E Nambara, Y-M Bi, and SJ Rothstein. OsPIN5b modulates rice (Oryza sativa) plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant Journal 83, 913-925, 2015. M Han, M Okamoto, PH Beatty, PH, SJ Rothstein and AG Good. The genetics of nitrogen use efficiency in crop plants. Annual Review of Genetics 49, DOI: 10.1146/annurev-genet-112414-055037, 2015. GN Nguyen, SJ Rothstein, G Spangenberg, G and S Kant. Role of microRNAs in plant response to nitrogen and phosphorous limiting conditions. Frontiers in Plant Science 6, doi 10.3389/fpls.2015.00629, 2015. V Yadav, I Molina, K Ranathunge, IQ Castillo, SJRothstein and JW Reed. ABCG transporters are required for suberin and pollen wall extracellular barriers in Arabidopsis. Plant Cell 26, 3569-3588, 2014. M Misyura, DR Guevara, S Subedi, D Hudson, PD McNicholas, J Colasantiand SJ Rothstein Nitrogen limitation and high density responses in rice suggest a role for ethylene under high density stress. BMC Genomics 15 doi:1186/1471-2164-15-681, 2014. SJ Rothstein; Y-M Bi, V Coneva, M Han and AG Good, The challenges of commercializing second-generation transgenic crop traits necessitate the development of international public sector research infrastructure.  Journal of Experimental Botany 65, 5673-5682, 2014. K Ranathunge, A El-Kereamy, S Gidda, Y-M Bi and SJ Rothstein. OsAMT1;1trangenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions. Journal of Experimental Botany 65, 965-979, 2014. Y-M Bi, A Meyer, GS Downs, X Shi, A El-Keramy, L Lukens and SJ Rothstein. High throughput sequencing of a hybrid maize and its parents shows different mechanisms responsive to nitrogen limitation. BMC Genomics 15, doi:10.1186/1471-2164-15-77, 2014. DR Guevara, A El-Kereamy, MW Yaish, Y-M Bi and SJ Rothstein. Functional characterization of the rice UDP-glucose 4 epimerase 1, OsUGE1: A potential role in cell wall carbohydrate partitioning during limiting nitrogen conditions. PloS ONE 9 e96158, 2014. JL Ericson, J Ziegler, DR Guevara, S Abel, RB Klosgen, J Mathur, SJ Rothstein, and MH SchattatAgrobacterium-derived cytokinin influences plastid morphology and starch accumulation in Nicotiana benthamiana during transient assays. BMC Plant Biology 14, 127.109, 2014. C Liseron-Monfils, Y-M Bi, GS Downs, W Wu, T Signorelli, G Lu, X Chen, J Colasanti, SJ Rothstein and M Raizada. Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs. Plant Signaling and Behavior 8, e26056, 2013. D Hudson, DR Guevara, AJ Hand, Z Xu, L Hao, X Chen, T Zhu, Y-M Bi, and SJ Rothstein. Rice cytokinin GATA transcription factor1 regulates chloroplast development and plant architecture. Plant Physiology 162, 132-144, 2013. Downs, GS, Bi, Y-M., Colasanti, J., Wu, W., Chen, X., Zhu, T., Rothstein, SJ, and Lukens, L. A developmental transcriptional network for Zea mays defines coexpression modules Plant Physiology, pp112.213231 2013. Humbert, S, Subedi, S, Cohn, J, Zeng, B., Bi, Y-M., Chen, X., McNicholas, PD, and Rothstein, SJ Genome-wide expression profiling of maize in response to individual and combined water and nitrogen stresses BMC Genomics 14: doi:10.1186/1471-2164-14-3, 2013. Misyura, M., Colasanti, J and Rothstein, SJ Physiological and genetic analysis of Arabidopsis anthocyanin mutants under chronic adverse environmental J Experimental Botany 64: 229-240. doi:10, 1093/jxb/ers328106, 2013. Tian, G, Lu, Q., Kohalmi, SE, Rothstien, SJ and Cui, Y. Evidence that the Arabidopsis Ubiquitin C-terminal Hydorlases 1 and 2 assemble with the 26S proteasome and the TREX-2 complex. Plant Signaling and Behavior 7: 1415-1419, 2012. Xurong Tang, Shaomin Bian, Mingjuan Tang, Qing Lu, Shengben Li, Xigang Liu, Gang Tian, Vi Nguyen, Edward W. T. Tsang, Aiming Wang, Steven J. Rothstein, Xuemei Chen, Yuhai Cui. MicroRNA–Mediated Repression of the Seed Maturation Program during Vegetative Development in Arabidopsis. pLOS Genetics 8(11): e1003091. Doi10.1371, 2012. Kant, S., Senaweera, S., Rodin, J, Materne, M., Burch, D, Rothstein, SJ, Spangernberg, G. Improving yield potential in corps under elevated CO2: integrating photosynthetic and nitrogen utilization efficiencies. Frontiers in Plant Sciences; 3: 162; doi: 10.3389/fpls, 2012.