个人简介

Ph.D. Chinese Academy of Agricultural Sciences 1995, BSc, Peking University 1991

研究领域

Developmental Biology Molecular Biology Physiology Plant Biology Proteomics Systems Biology

Protein homeostatic processes include protein synthesis, folding, stabilization of conformation, refolding of misfolded proteins and clearance of misfolded proteins inside the cell. To maintain a proper cellular protein homeostasis is important for all living organisms during normal growth and under stress conditions. My laboratory is interested in the mechanisms of different cellular protein quality control systems that are players for protein homeostasis. Specifically, we are interested in the mechanism of action of HSP90 family molecular chaperones and investigate how HSP90 proteins are involved in plant development, organelle function, and stress resistance by using Arabidopsis as a model organism. We use molecular, biochemical, proteomic, and genetic approaches to study the role of HSP90s and their interacting partners in Arabidopsis. Our research focuses on how HSP90 helps its client proteins to achieve their proper physiological functions during organelle biogenesis and normal physiological conditions. Additionally, we are interested in the mechanisms of regulated cellular protein degradation pathways. We use biochemical, genetic and proteomic approaches to study how certain proteins are selectively degraded by the 26S proteasome in a ubiquitin-independent pathway, by using baking yeast as a model organism.

近期论文

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A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis.Zhang M, Wang C, Lin Q, Liu A, Wang T, Feng X, Liu J, Han H, Ma Y, Bonea D, Zhao R, Hua X.Plant J. 2015 Aug;83(4):582-599 pubmed A highly charged region in the middle domain of plant endoplasmic reticulum (ER)-localized heat-shock protein 90 is required for resistance to tunicamycin or high calcium-induced ER stresses.Chong LP, Wang Y, Gad N, Anderson N, Shah B, Zhao R.J. Exp. Bot. 2015 Jan;66(1):113-24 pubmed Cosuppression of the chloroplast localized molecular chaperone HSP90.5 impairs plant development and chloroplast biogenesis in Arabidopsis.Oh SE, Yeung C, Babaei-Rad R, Zhao R.BMC Res Notes 2014;7:643 pubmed The stability of the small nucleolar ribonucleoprotein (snoRNP) assembly protein Pih1 in Saccharomyces cerevisiae is modulated by its C terminus.Paci A, Liu XH, Huang H, Lim A, Houry WA, Zhao R.J. Biol. Chem. 2012 Dec;287(52):43205-14 pubmed Structure of minimal tetratricopeptide repeat domain protein Tah1 reveals mechanism of its interaction with Pih1 and Hsp90.Jiménez B, Ugwu F, Zhao R, Ortí L, Makhnevych T, Pineda-Lucena A, Houry WA.J. Biol. Chem. 2012 Feb;287(8):5698-709 pubmed Expression of five AtHsp90 genes in Saccharomyces cerevisiae reveals functional differences of AtHsp90s under abiotic stresses.Song H, Fan P, Shi W, Zhao R, Li Y.J. Plant Physiol. 2010 Sep;167(14):1172-8 pubmed Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity.Zhao R, Leung E, Grüner S, Schapira M, Houry WA.PLoS ONE 2010;5(4):e9934 pubmed Overexpression of AtHsp90.2, AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses.Song H, Zhao R, Fan P, Wang X, Chen X, Li Y.Planta 2009 Mar;229(4):955-64 pubmed Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation.Zhao R, Kakihara Y, Gribun A, Huen J, Yang G, Khanna M, Costanzo M, Brost RL, Boone C, Hughes TR, Yip CM, Houry WA.J. Cell Biol. 2008 Feb;180(3):563-78 pubmed Expression of a constitutively activated plasma membrane H+-ATPase alters plant development and increases salt tolerance.Gévaudant F, Duby G, von Stedingk E, Zhao R, Morsomme P, Boutry M.Plant Physiol. 2007 Aug;144(4):1763-76 pubmed Molecular interaction network of the Hsp90 chaperone system.Zhao R, Houry WA.Adv. Exp. Med. Biol. 2007;594:27-36 pubmed