个人简介

Ph.D. Institute of Marine Biology, Vladivostok, Russia

研究领域

Animal Biology Cell Biology Developmental Biology Quantitative Biology

Cell migration is central in a wide range of physiological and pathological processes, including development, wound healing and cancer metastasis. In the animal, cell migration is governed by a variety of extracellular cues that control activity of specific signaling pathways and regulate cytoskeletal dynamics. These cues are diverse in nature and include biochemical gradients, mechanical forces, and gradients of extracellular matrix stiffness and topology. Although several major signaling pathways have been previously implicated as master regulators of cell migration guided by the biochemical cues, the mechanisms by which cells sense mechanical stimuli and transduce them into cellular responses, such as migration, are largely unknown. We aim to uncover how migration of animal cells is controlled by mechanical signals form the extracellular environment. Our lab uses a multifaceted experimental approach, combining cell biology, molecular biology, and biophysics techniques with high-resolution quantitative optical microscopy to address the following specific questions: (i) unraveling molecular mechanisms that transduce physical signals experienced by cells into activity of signaling pathways, (ii) assess contribution of directed cell migration guided by physical cues to pathological conditions, (iii) understanding how mechanosensitive cellular responses are modulated by chemical guidance cues, such as spatial gradients of growth factors.

近期论文

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Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly.Skau CT, Plotnikov SV, Doyle AD, Waterman CM.Proc. Natl. Acad. Sci. U.S.A. 2015 Apr; pubmed Rac1-dependent phosphorylation and focal adhesion recruitment of myosin IIA regulates migration and mechanosensing.Pasapera AM, Plotnikov SV, Fischer RS, Case LB, Egelhoff TT, Waterman CM.Curr. Biol. 2015 Jan;25(2):175-86 pubmed High-resolution traction force microscopy.Plotnikov SV, Sabass B, Schwarz US, Waterman CM.Methods Cell Biol. 2014;123:367-94 pubmed Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth.Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, Waterman CM.J. Cell Biol. 2013 Jul;202(1):163-77 pubmed Guiding cell migration by tugging.Plotnikov SV, Waterman CM.Curr. Opin. Cell Biol. 2013 Oct;25(5):619-26 pubmed Force fluctuations within focal adhesions mediate ECM-rigidity sensing to guide directed cell migration.Plotnikov SV, Pasapera AM, Sabass B, Waterman CM.Cell 2012 Dec;151(7):1513-27 pubmed Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure.Chen X, Nadiarynkh O, Plotnikov S, Campagnola PJ.Nat Protoc 2012 Apr;7(4):654-69 pubmed Cardiac fibroblasts require focal adhesion kinase for normal proliferation and migration.Manso AM, Kang SM, Plotnikov SV, Thievessen I, Oh J, Beggs HE, Ross RS.Am. J. Physiol. Heart Circ. Physiol. 2009 Mar;296(3):H627-38 pubmed Measurement of muscle disease by quantitative second-harmonic generation imaging.Plotnikov SV, Kenny AM, Walsh SJ, Zubrowski B, Joseph C, Scranton VL, Kuchel GA, Dauser D, Xu M, Pilbeam CC, Adams DJ, Dougherty RP, Campagnola PJ, Mohler WA.J Biomed Opt 2008 Jul-Aug;13(4):044018 pubmed Second harmonic generation imaging microscopy studies of osteogenesis imperfecta.Nadiarnykh O, Plotnikov S, Mohler WA, Kalajzic I, Redford-Badwal D, Campagnola PJ.J Biomed Opt 2007 Sep-Oct;12(5):051805 pubmed Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres.Plotnikov SV, Millard AC, Campagnola PJ, Mohler WA.Biophys. J. 2006 Jan;90(2):693-703 pubmed Optical clearing for improved contrast in second harmonic generation imaging of skeletal muscle.Plotnikov S, Juneja V, Isaacson AB, Mohler WA, Campagnola PJ.Biophys. J. 2006 Jan;90(1):328-39 pubmed