个人简介

Ph.D. University of Toronto 2000 M.Sc. University of Manitoba 1996

研究领域

Cell Biology

As a cell biologist, our lab studies the inner workings of immune cells called macrophages. Macrophages are crucial frontline cells in the body’s defense against infection. We also study the bone cells: osteoclasts and osteoblasts. We make regular use of cell culture, biochemistry and molecular biology to manipulate these cells in vitro. We analyze the cells using current fluorescent imaging techniques including spinning disk confocal, TIRF imaging and electron microscopy for high resolution understanding of morphological events at the cell surface. We are interested in how macrophages take up bacteria and destroy them intracellularly. We are also studying how some pathogens like Chlamydia trachomatis manipulate host cells to gain a bacterial advantage. We study how bone formation and bone loss is accomplished by osteoblasts and osteoclasts, respectively. Of interest we study how osteoarthritis , rheumatoid arthritis and the extreme effects of a microgravity environment influence the bone cells to cause net bone loss. Our work to date has been funded by NSERC, CIHR, CIHR New Emerging Team and Canadian Space Agency grants. Our laboratory is within the Science Wing at the University of Toronto Scarborough. As a member of the Centre for the Neurobiology of Stress facility, we have access to state-of-the-art equipment facilities and services, including cell culture facilities, imaging facilities (including confocal microscopy, scanning and transmission electron microscopy) and flow cytometry facilities, etc. The CNS is the result of a $10 million CFI award to UTSC and all of my trainees have full-time access to this facility.

近期论文

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Modulation of osteoclastogenesis with macrophage m1- and m2-inducing stimuli.Jeganathan S, Fiorino C, Naik U, Sun HS, Harrison RE.PLoS ONE 2014;9(8):e104498 pubmed EB1 levels are elevated in ascorbic Acid (AA)-stimulated osteoblasts and mediate cell-cell adhesion-induced osteoblast differentiation.Pustylnik S, Fiorino C, Nabavi N, Zappitelli T, da Silva R, Aubin JE, Harrison RE.J. Biol. Chem. 2013 Jul;288(30):22096-110 pubmed Monocytes from patients with osteoarthritis display increased osteoclastogenesis and bone resorption: the In Vitro Osteoclast Differentiation in Arthritis study.Durand M, Komarova SV, Bhargava A, Trebec-Reynolds DP, Li K, Fiorino C, Maria O, Nabavi N, Manolson MF, Harrison RE, Dixon SJ, Sims SM, Mizianty MJ, Kurgan L, Haroun S, Boire G, de Fatima Lucena-Fernandes M, de Brum-Fernandes AJ.Arthritis Rheum. 2013 Jan;65(1):148-58 pubmed Rab GTPase mediated procollagen trafficking in ascorbic acid stimulated osteoblasts.Nabavi N, Pustylnik S, Harrison RE.PLoS ONE 2012;7(9):e46265 pubmed Chlamydia trachomatis vacuole maturation in infected macrophages.Sun HS, Eng EW, Jeganathan S, Sin AT, Patel PC, Gracey E, Inman RD, Terebiznik MR, Harrison RE.J. Leukoc. Biol. 2012 Oct;92(4):815-27 pubmed Classically activated macrophages use stable microtubules for matrix metalloproteinase-9 (MMP-9) secretion.Hanania R, Sun HS, Xu K, Pustylnik S, Jeganathan S, Harrison RE.J. Biol. Chem. 2012 Mar;287(11):8468-83 pubmed Chlamydia trachomatis inclusions induce asymmetric cleavage furrow formation and ingression failure in host cells.Sun HS, Wilde A, Harrison RE.Mol. Cell. Biol. 2011 Dec;31(24):5011-22 pubmed Effects of microgravity on osteoclast bone resorption and osteoblast cytoskeletal organization and adhesion.Nabavi N, Khandani A, Camirand A, Harrison RE.Bone 2011 Nov;49(5):965-74 pubmed Measuring immune receptor mobility by fluorescence recovery after photobleaching.Silver K, Harrison RE.Methods Mol. Biol. 2011;748:155-67 pubmed Kinesin 5B is necessary for delivery of membrane and receptors during FcγR-mediated phagocytosis.Silver KE, Harrison RE.J. Immunol. 2011 Jan;186(2):816-25 pubmed Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S.J. Cell Biol. 2010 Dec;191(6):1205-18 pubmed