个人简介

Ph.D. University of Toronto 1990 M.Sc, McGill University 1985 B.Sc. Trent University 1983

研究领域

Cell Biology Molecular Biology Plant Biology

One area that my research group investigates is the signalling systems regulating compatible and self-incompatible pollen responses in the pistil. These are critical recognition systems for selecting which pollen grains will be accepted or rejected, and thus, selecting optimal pollen grains for fertilization. My research is focused on the complex intracellular signalling takes place in the stigma to identify and accept compatible pollen while rejecting self-incompatible pollen and ignoring foreign pollen. In a search for factors that act early in the compatible pollen responses, my research group identified the Exo70A1 exocyst subunit as a critical component in the stigma to promote pollen hydration and pollen tube growth and are currently exploring this model in more detail. In contrast to the compatible pollen recognition system is the self-incompatibility system which prevents self-fertilization and inbreeding. In the self-incompatibility response, a stigma-specific S Receptor Kinase (SRK) is responsible for binding the SP11/SCR ligand from the self-incompatible pollen and activates a signal transduction pathway in the stigmatic papilla to reject this pollen. My research group identified three proteins which are involved in this signalling pathway, THL1, THL2 and ARC1. THL1 and THL2 belong to the thioredoxin h family and act as negative regulators of SRK. ARC1 is an E3 ubiquitin ligase which is activated downstream of SRK and is proposed to inhibit compatibility factors such as Exo70A1 causing pollen rejection. My research is currently exploring in more detail how these events leads to pollen rejection.

近期论文

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Pollen Acceptance or Rejection: A Tale of Two Pathways.Doucet, Jennifer, Hyun Kyung Lee, and Daphne R Goring. 2016. Trends in plant science, no. 12 (November 11). pubmed Dominance modifier: Expanding mate options.Goring, Daphne R. 2016. Nature plants (December 22). pubmed Following the Time-Course of Post-pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations.Safavian, Darya, Jennifer Doucet, and Daphne R Goring. 2016. Methods in molecular biology (Clifton, N.J.) pubmed Yeast two-hybrid interactions between Arabidopsis lyrata S Receptor Kinase and the ARC1 E3 ligase.Indriolo E, Goring DR.Plant Signal Behav 2016 May; pubmed RNA Silencing of Exocyst Genes in the Stigma Impairs the Acceptance of Compatible Pollen in Arabidopsis.Safavian D, Zayed Y, Indriolo E, Chapman L, Ahmed A, Goring DR.Plant Physiol. 2015 Dec;169(4):2526-38 pubmed PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana.Humphrey TV, Haasen KE, Aldea-Brydges MG, Sun H, Zayed Y, Indriolo E, Goring DR.J. Exp. Bot. 2015 Jan;66(1):71-83 pubmed Autophagy in the rejection of self-pollen in the mustard family.Safavian D, Goring D.Autophagy 2014;10(12):2379-80 pubmed Reversible ubiquitylation in plant biology.Fu H, Goring DR, Genschik P.Front Plant Sci 2014;5:707 pubmed The ARC1 E3 ligase promotes a strong and stable self-incompatibility response in Arabidopsis species: response to the Nasrallah and Nasrallah commentary.Goring DR, Indriolo E, Samuel MA.Plant Cell 2014 Oct;26(10):3842-6 pubmed High humidity partially rescues the Arabidopsis thaliana exo70A1 stigmatic defect for accepting compatible pollen.Safavian D, Jamshed M, Sankaranarayanan S, Indriolo E, Samuel MA, Goring DR.Plant Reprod 2014 Sep;27(3):121-7 pubmed A conserved role for the ARC1 E3 ligase in Brassicaceae self-incompatibility.Indriolo E, Goring DR.Front Plant Sci 2014;5:181 pubmed The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis.Indriolo E, Safavian D, Goring DR.Plant Cell 2014 Apr;26(4):1525-1543 pubmed