个人简介
I first discovered the beauty and elegance of phages during my Ph.D. studies, which were focused on the DNA packaging enzyme of E. coli phage lambda. During my post-doctoral work at MIT, I switched to the field of protein structure and folding. I continued this work in my own lab investigating the thermodynamics and kinetics of folding of the SH3 domain. I have also worked extensively on the functioning of SH3 domains in budding yeast. Luckily, as time went on in my own lab, I was able to move back into the phage field.
研究领域
My laboratory focuses on bacteriophages (phages), the viruses that infect bacteria. We are interested in:
How phage particles assemble. For these studies we combine techniques of structural biology (X-ray crystallography, NMR, and electron microscopy) with molecular biology and in vivo studies.
How phage genomes found within bacterial genomes (prophages) alter the physiology of the host bacteria. We pursue this work primarily in the pathogenic bacteria, Pseudomonas aeruginosa. We seek to understand how prophages affect virulence and pathogenesis of this organism. This work is relevant to Cystic Fibrosis patients in whom P. aeruginosa is a major cause of illness.
Phage-related entities encoded in bacterial genomes. These entities, such as R- and F-pyocins in P. aeruginosa or Photorhabdus Virulence Cassettes in other species, can mediate killing of other bacterial species or eukaryotic cells. Little is known of how these entities function.
CRISPR-Cas systems. CRISPR-Cas systems are an adaptive immunity system in bacteria. They operate in a manner similar to RNAi in eukaryotes and are widespread in both bacteria and archaea. Recently CRISPR-Cas systems have been adapted for genome editing in a wide variety of species including humans. We discovered phage-encoded genes that inhibit the CRISPR-Cas systems of P. aeruginosa, and we are currently studying the prevalence of these anti-CRISPR systems and how they work.
A major goal of my research program is to provide a fruitful training ground for my students where they can gain experience in as many techniques as possible, and develop independent projects based on their own interests and strengths.
近期论文
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A new group of phage anti-CRISPR genes inhibits the type I-E CRISPR-Cas system of Pseudomonas aeruginosa
Pawluk A, Bondy-Denomy J, Cheung VH, Maxwell KL, Davidson AR.
MBio 25, e00896 (2014) Read
HNH proteins are a widespread component of phage DNA packaging machines
Kala S, Cumby N, Sadowski PD, Hyder BZ, Kanelis V, Davidson AR, Maxwell KL
Proc Natl Acad Sci U S A. 111, 6022-6027 (2014) Read
Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system
Bondy-Denomy, J., Pawluk, A., Maxwell, K.L. & Davidson, A.R.
Nature 493, 429–432 (2013) Read
Structural and functional studies of gpX of Escherichia coli phage P2 reveal a widespread role for LysM domains in the baseplates of contractile-tailed phages
Maxwell KL, Fatehi Hassanabad M, Chang T, Paul VD, Pirani N, Bona D, Edwards AM, Davidson AR
J Bacteriol. 195, 5461-5468 (2013) Read
Tail tip proteins related to bacteriophage λ gpL coordinate an iron-sulphur cluster
Tam, W., Pell, L.G., Bona, D., Tsai1, A., Dai, X.X., Hendrix, R.W., Maxwell, K.L., and Davidson, A.R.
J. Mol. Biol. 425, 2450–2462 (2013) Read
The CRISPR/Cas Adaptive Immune System of Pseudomonas aeruginosa Mediates Resistance to Naturally Occurring and Engineered Phages
Cady, K.C., Bondy-Denomy, J., Heussler, G.E., Davidson, A.R. & O'Toole, G.A.
J Bacteriol. 194, 5728-5738 (2012) Read
Assembly mechanism is the key determinant of the dosage sensitivity of a phage structural protein.
Cardarelli. L., Maxwell, K.L., Davidson, A.R.
Proc Natl Acad Sci U S A 108, 10168-10173 (2011) Read
Phages have adapted the same protein fold to fulfill multiple functions in virion assembly
Cardarelli, L., Pell, L.G., Neudecker, P., Pirani, N., Liu, A., Baker, L.A., Rubinstein, J.L., Maxwell, K.L., Davidson, A.R.
Proc Natl Acad Sci U S A 107, 14384-14389 (2010) Read
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