Stagljar, Igor - University of Toronto - Department of Biochemistry

个人简介

Igor received his Ph.D. in Molecular Biology from ETH Zurich in Switzerland, where he worked in Markus Aebi’s lab studying protein glycosylation in yeast and humans. His postdoctoral fellowship was at the University of Zurich with Walter Schaffner and Ulrich Huebscher, where he studied RNA transcription and DNA repair. In addition, Igor was a visiting scientist at the University of Washington in Seattle with Stan Fields, the inventor of the yeast two-hybrid technology. Igor was Assistant Professor at the University of Zurich from 2002-2005, Associate Professor at the University of Toronto since 2005, and Professor since 2010.

研究领域

A key focus of the Stagljar lab is to understand the function of a wide range of pharmaceutically relevant yeast and human integral membrane proteins involved in cell signaling and membrane transport at a systems level. Despite extensive research in the past decade, there is a lack of in-depth understanding of protein networks associated with these integral membrane proteins because of their unique biochemical features, enormous complexity and multiplicity. This is a major obstacle for designing improved and more targeted therapies, and importantly, understanding the biology of deregulation of these integral membrane proteins which leads to numerous human diseases. Our lab is internationally known for the development of the split-ubiquitin Membrane Yeast Two-Hybrid (MYTH) and Mammalian Membrane Two-Hybrid (MaMTH) technologies, powerful tools for the identification of interactors of a given integral membrane protein and one of the key interactive proteomic technologies available to researchers today. To date, MYTH and MaMTH have lead to many groundbreaking discoveries, including the elucidation of functions of various integral membrane proteins involved in human health and disease. Currently, there are several large-scale, ongoing projects in the lab aimed towards elucidating how yeast and human integral membrane proteins and their interacting partners lead to either diseased or healthy states. These studies include mapping of protein interaction networks of various families of membrane proteins such as ABC Transporters, Receptor Tyrosine Kinases, G-protein Coupled Receptors, Cancer Stem Cell Receptors and Ion Channels. Subsequently, proteins and pathways identified by our initial protein interaction screens are studied in depth using various genetic, molecular, and biochemical approaches, shedding light on cell signaling and membrane transport pathways, which control cell behavior in normal and disease cells at a systems level. In addition, because the Stagljar group studies ‘druggable’ membrane proteins, they hope their work will unveil new avenues for diagnosis and treatment of many human diseases.

近期论文

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A Comprehensive Membrane Interactome Mapping of Sho1p Reveals Fps1p as a Novel Key Player in the Regulation of the HOG Pathway in S. cerevisiae Lam, M.H.Y, Snider, J., Rehal, M., Wong, V., Aboualizadeh, F., Drecun, L., Wong, O., Jubran, B., Li, M., Ali, M., Jessulat, M., Deineko, V., Miller, R., Lee, M., Park, H-O., Davidson, A., Babu, M., and Stagljar, I. J Mol Biol. 2015 Jun 5;427(11):2088-103. doi: 10.1016/j.jmb.2015.01.016. Epub 2015 Jan 30. Read Application Guide for OMICs Approaches to Cell Signaling Yao, Z., Petschnigg, J., Ketteler, R., and Stagljar, I. Nat Chem Biol. 2015 Jun;11(6):387-97. doi: 10.1038/nchembio.1809. Epub 2015 May 15 Read Fundamentals of protein interaction mapping Snider, J., Kotlyar, M., Saraon, P., Yao, Z., Jurisica, I., and Stagljar, I. Mol Syst Biol. 2015 Dec; 11(12): 848. Published online 2015 Dec 17. doi: 10.15252/msb.20156351 Read The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells Petschnigg J, Groisman B, Kotlyar M, Taipale M, Zheng Y, Kurat CF, Sayad A, Sierra JR, Mattiazzi Usaj M, Snider J, Nachman A, Krykbaeva I, Tsao MS, Moffat J, Pawson T, Lindquist S, Jurisica I, Stagljar I. Nat Methods. 2014 May;11(5):585-92. doi: 10.1038/nmeth.2895. Epub 2014 Mar 23. Read Mapping the functional yeast ABC transporter interactome Snider J, Hanif A, Lee ME, Jin K, Yu AR, Graham C, Chuk M, Damjanovic D, Wierzbicka M, Tang P, Balderes D, Wong V, Jessulat M, Darowski KD, San Luis BJ, Shevelev I, Sturley SL, Boone C, Greenblatt JF, Zhang Z, Paumi CM, Babu M, Park HO, Michaelis S, Stagljar I. Nat Chem Biol. 2013 Sep;9(9):565-72. doi: 10.1038/nchembio.1293. Epub 2013 Jul 7. Read Regulation of CD133 by HDAC6 promotes β-catenin signaling to suppress cancer cell differentiation Mak AB, Nixon AM, Kittanakom S, Stewart JM, Chen GI, Curak J, Gingras AC, Mazitschek R, Neel BG, Stagljar I, Moffat J. Cell Rep. 2012 Oct 25;2(4):951-63. doi: 10.1016/j.celrep.2012.09.016. Epub 2012 Oct 19. Read Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae Babu M, Vlasblom J, Pu S, Guo X, Graham C, Bean BD, Burston HE, Vizeacoumar FJ, Snider J, Phanse S, Fong V, Tam YY, Davey M, Hnatshak O, Bajaj N, Chandran S, Punna T, Christopolous C, Wong V, Yu A, Zhong G, Li J, Stagljar I, Conibear E, Wodak SJ, Emili A, Greenblatt JF. Nature. 2012 Sep 27;489(7417):585-9. doi: 10.1038/nature11354. Epub 2012 Sep 2. Read Regulation of epidermal growth factor receptor trafficking by lysine deacetylase HDAC6 Deribe YL, Wild P, Chandrashaker A, Curak J, Schmidt MH, Kalaidzidis Y, Milutinovic N, Kratchmarova I, Buerkle L, Fetchko MJ, Schmidt P, Kittanakom S, Brown KR, Jurisica I, Blagoev B, Zerial M, Stagljar I, Dikic I. Sci Signal. 2009 Dec 22;2(102):ra84. doi: 10.1126/scisignal.2000576. Read