研究领域

Analytical Chemistry

The research in my group focuses on the chemical and physical basis of migration mechanisms for biopolymers and colloids as model systems. Tailored micro- and nanofluidic devices are developed wherein migration processes of biomolecules (e.g. DNA, proteins) are studied by optical single molecule as well as ensemble techniques. A second focus lies on the development of micro- and nanofluidic devices as platforms for novel single cell analysis tools. The ultimate goal consists in elucidating novel bioanalytical and biotechnological tools as well as efficient lab-on-a-chip systems for proteom research and systems nanobiology. Migration mechanisms of biomolecules are studied via fluorescence video microscopy with subsequent particle tracking and on-chip separation experiments. A major interest lies in the dielectrophoretic behaviour of biomolecules which can be advantageously used in separation experiments. A very important part of our work is dedicated to adequate surface design, providing not only for the control of electrokinetic properties in the micro-and nanodevices but also as an additional design tool for novel migration mechanisms. Soft lithography serves as a primary fabrication method for tailored microdevices, whereas other appropriate micro- and nanofabrication methods find application for specific structuring problems. Our single cell studies focus on biologically relevant questions in organisms such as insect cells and bacteria. Single cell manipulation is developed for reproducible and parallel cell positioning. Our work also includes the integration of ultra sensitive detection methods in the microfluidic chip such as laser induced fluorescence as well as off-chip coupling to sensitive state of the art detection methods.

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A. Nakano, F. Camacho-Alanis, A. Ros, Insulator-Based Dielectrophoretisis with Proteins in Nanostructured Devices, Analyst (2014) in press, DOI: 10.1039/C4AN01503G F. Camacho-Alanis, A. Ros, Protein Dielectrophoresis and the Link to Dielectric Properties, Bioanalysis (2014) in press A. Nakano, F. Camacho-Alanis, A. Ros, Insulator-Based Dielectrophoretic Behavior of β-Galactosidase under DC and Low Frequency AC Conditions, 18th µTAS Proceedings (2014) 2468-2470 B. G. Abdallah, M. Sawtelle, A. Ros, High Throughput Nanoparticle Sorting for Serial Femtosecond Crystallography, 18th µTAS Proceedings (2014) 1305-1307 A. Nakano, J. Luo, A. Ros, Temporal and Spatial Temperature Measurement in Insulator-based Dielectrophoretic Devices, Analytical Chemistry (2014) 86, 6516−6524 DOI:10.1021/ac501083h J. Luo, B. G. Abdallah, G. G. Wolken, E. A. Arriaga, A. Ros, Insulator-based dielectrophoresis of mitochondria, Biomicrofluidics (2014) 8, 021801 DOI: 10.1063/1.486685 S. Bhattacharya, T.-C. Chao, N. Ariyasinghe, Y. Ruiz, D. Lake, R. Ros, A. Ros, Selective trapping of single mammalian breast cancer cells by insulator-based dielectrophoresis, Analytical and Bioanalytical Chemistry (2014) 406:1855–1865 DOI:10.1007/s00216-013-7598-2 W. Christenson, I. Yermolenko, B. Plochberger, F. Camacho-Alanis, A. Ros, T.P. Ugarova, R. Ros, Combined single cell AFM manipulation and TIRFM for probing the molecular stability of multilayer fibrinogen matrices Ultramicroscopy (2014) 136, 211-215 DOI: 10.1016/j.ultramic.2013.10.009 C. Kupitz et al., Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laserNature (2014) 513, 261-265 DOI:10.1038/nature13453 B. G. Abdallah, C. Kupitz, P. Fromme, A. Ros, Crystallization of the Large Membrane Protein Complex Photosystem I in a Microfluidic Channel ACS Nano (2013) 7, 9129-9137 DOI: 10.1021/nn402515q B.G. Abdallah, T.-C. Chao, C. Kupitz, P. Fromme, A. Ros, Dielectrophoretic Sorting of Membrane Protein Nanocrystals ACS Nano (2013) 7, 10534–10543, DOI: 10.1021/nn403760q L. Gan, T.-C. Chao, F. Camacho-Alanis, A. Ros, Six Helix Bundle and Triangle DNA Origami Insulator- Based Dielectrophoresis Analytical Chemistry (2013) 85, 11427-11434, DOI: 10.1021/ac402493u J.-U. Kreft, C. M. Plugge, V. Grimm, C. Prats, J. H. J. Leveau, T. Banitz, S. Baines, J. Clark, A. Ros, I. Klapper, C. J. Topping, A. J. Field, A. Schuler, E. Litchman, F. L. Hellweger, Mighty small: Observing and Modeling Individual Microbes Becomes Big Science, PNAS (2013) 110, 18027–18028 DOI: 10.1073/pnas.1317472110 M. Yang, T.-C. Chao, R. Nelson, A. Ros, Protein identification and quantification for single cell analysis by coupling a microfluidic platform with MALDI-TOF, 17th µTAS Proceedings(2013) 1039-1041 A. Nakano, A. Ros, Protein Dielectrophoresis: Advances, Challenges and Applications, Electrophoresis (2013) 34, 1085–1096 DOI: 10.1002/elps.201200482 R. Martinez-Duarte, F. Camacho-Alanis, P. Renaud, A. Ros, Dielectrophoresis of DNA using 3D Carbon Electrodes, Electrophoresis (2013) 34, 1113-1122 DOI: 10.1002/elps.201200447 B. Abdallah, A. Ros, Surface coatings for microfluidic-based medical devices, Microfluidic devices for biomedical applications, XiuJun Li & Yu Zhou (Editors), Woodhead Publishing (2013) ISBN-13: 978 0 85709 697 5