Sharpe, Simon - University of Toronto - Department of Biochemistry

个人简介

Simon Sharpe obtained his BSc in Cell Biology and Biochemistry at the Memorial University of Newfoundland in 1997. After obtaining a PhD from the biochemistry department of the University of Western Ontario in 2002, he joined Robert Tycko’s group at the NIH as a postdoctoral fellow – using solid state NMR to determine the structures of peptide-antibody complex and integral membrane proteins involved in the pathogenesis of HIV-1. Dr. Sharpe joined the program in Molecular Structure and Function at The Hospital for Sick Children and the Department of Biochemistry in 2006, where his research interests include self-assembling proteins (amyloid formation in degenerative diseases, elastomeric proteins, and others), protein-membrane interactions and integral membrane proteins.

研究领域

Research in the Sharpe lab focuses on mechanisms of peptide/protein self-assembly, and the links between sequence, structure, and activity of the resulting macromolecular assemblies. We use a set of biophysical tools, which an emphasis on applying solid state and solution NMR spectroscopy to achieve a molecular-level understanding of protein structure and dynamics. For this purpose, the Sharpe lab has dedicated use of 500, 600 and 700 MHz NMR spectrometers fully equipped for biomolecular solution and solid state NMR experiments.

近期论文

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The mechanism of membrane disruption by cytotoxic amyloid oligomers formed by prion protein(106-126) is dependent on bilayer composition. Walsh P, Vanderlee G, Yau J, Campeau J, Sim VL, Yip CM, Sharpe S J Biol Chem. 2014 289:10419-10430 Read Conformational transitions of the cross-linking domains of elastin during self-assembly Reichheld SE, Muiznieks LD, Stahl R, Simonetti K, Sharpe S, Keeley FW J Biol Chem. 2014 289:10057-10068 Read Dynamic equilibria between monomeric and oligomeric misfolded states of the mammalian prion protein measured by 19F NMR Larda ST, Simonetti K, Al-Abdul-Wahid MS, Sharpe S, Prosser RS J Am Chem Soc. 2013 135:10533-10541 Read Structures of amyloid fibrils formed by the prion protein derived peptides PrP(244-249) and PrP(245-250) Yau J, Sharpe S J Struct Biol. 2012 180:290-302 Read Dimerization of the transmembrane domain of human tetherin in membrane mimetic environments Cole G, Simonetti K, Ademi I, Sharpe S Biochemistry 2012 51:5033-5040 Read Probing dynamic conformations of the high-molecular-weight αB-crystallin heat shock protein ensemble by NMR spectroscopy Baldwin AJ, Walsh P, Hansen DF, Hilton GR, Benesch JL, Sharpe S, Kay LE J Am Chem Soc. 2012 134:15343-15350 Read Structure of an intermediate state in protein folding and aggregation Neudecker P, Robustelli P, Cavalli A, Walsh P, Lundström P, Zarrine-Afsar A, Sharpe S, Vendruscolo M, Kay LE Science 2012 336:362-366 Read Solid-State NMR characterization of autofluorescent fibrils formed by the elastin-derived peptide GVGVAGVG Sharpe S, Simonetti K, Yau J, Walsh P Biomacromolecules 2011 12:1546-1555 Read