研究领域

Analytical Chemistry/Biochemistry/Inorganic & Materials Chemistry/Organic Chemistry/Physical Chemistry

My research is focused on developing new technology for ultrasmall volume biological fluids and tissue analysis. New technologies will allow the full chemical and bioactive analysis of incredibly small samples—on the order of a few nanoliters or a cube about one-tenth the diameter of a human hair. The idea of these ultrasmall volume biological assays opens the door to a wide variety of revolutionary applications, including inexpensive disposable clinical assays chips, implantable micro health monitoring systems, millions of parallel assays from a single microscopic sample (proteomic and genomic application—leading to personalized medical care), the ability to chemically map tissues at high spatial resolution, non-invasive sampling and local disease treatment among other interesting applications. To develop and apply these new technologies, our group’s research interests span chemistry, physics, biochemistry, engineering, medical science and biology. Much of the details of accomplishing our tasks lie in fundamental issues including surface chemistry, materials design, micro- and nanofluidics, dynamic interfacial physics & chemistry, and microfluidic/microelectronic chip design and fabrication. One long-term goal is provide truly predictive pattern recognition for early disease detection for individuals, and by definition, define various states of wellness. The earliest a disease can be detected is when the wellness state begins to falter. All of the bioanalytical technical advances can be related to developing the ability to map the detailed chemical patterns of an active biological system. This map includes the idea of pattern recognition in the sense of varying concentrations of ‘markers’ for specific disease states and pattern recognition of those concentrations over time. One of the biggest challenges in proposing to address early disease detection is defining quantitatively the baseline or normal fluctuations of the operating biological system. A clearly ideal starting point for developing these capabilities is the observation of established chemical markers of stress. Arguable, a system under stress is the first step away from wellness and toward disease. Our current projects focus on enabling the accurate and precise measurement of important bio-particles and molecules, which is a very difficult task. The huge variety and subtle differences between bio-particles and molecules presents a massive challenge to isolate and concentrate the important materials away from the unimportant. We have pioneered a new separation scheme enabled by the electronics industry fabrication strategies, resulting in micro and nanofluidics, where unheard of control of electric and flow fields can uniquely capture targets. We have demonstrated this on human and pathogen cells, along with a variety of important proteins. .

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Dielectrophoretic Differentiation and Separation of Staphylococcus based on Antibiotic Resistance. Paul V. Jones, Shannon Huey, Paige Davis, and Mark A. Hayes* Biophysical Journal, 2015, 108, in preparation. Sensitive Detection of Cardiac Biomarkers Using a Magnetic Microbead Immunoassay. Christine F. Woolley and Mark A. Hayes* Analyst 2015, 140, in preparation. Selective Concentration of Sindbis Virus with Gradient Insulator-based Dielectrophoresis. Jie Ding, Brenda G. Hogue, Robert Lawrence, and Mark A. Hayes* Analyst 2015, 140, in preparation. Localized Asymmetric Electric and Velocity Field Effects during Counterflow Gradient Focusing at a Converging Channel. Michael W. Keebaugh and Mark A. Hayes* Electrophoresis 2015, 36, submitted. Theoretical Limitations of Quantification for Noncompetitive Sandwich Immunoassays. Christine F. Woolley and Mark A. Hayes* Anal. Bioanal. 2015, 407(1), in revision. Development of the Resolution Theory for Gradient insulator-based Dielectrophoresis. Mark A. Hayes & Paul V. Jones Electrophoresis, 2015, 36, accepted February 2015. preprint Emerging Technologies for Biomedical Analysis. Christine F. Woolley and Mark A. Hayes* Analyst 2014, 139(10), 2277-2288 DOI: 10.1039/c4an00259h preprint Ground level environmental protein concentrations in various ecuadorian environments: potential uses of aerosolized protein for ecological research. Sarah J.R. Staton, Andrea Woodward, Josemar A. Castillo, Kelly Swing, Mark A. Hayes Ecological Indicators, 2014, 48, 389-395 DOI: 10.1016/j.ecolind.2014.08.036. article Development of the Resolution Theory for Electrophoretic Exclusion. Stacy M. Kenyon, Michael W. Keebaugh, & Mark A. Hayes Electrophoresis, 2014, 35, 2551-2559 DOI 10.1002/elps.201300572. article Simulation and visualization of velocity fields in simple electrokinetic devices. Prasun Mahanti*, Thomas Taylor, Douglas Cochran, Michael Keebaugh, and Mark A. Hayes Proceedings of Visualization and Data Analysis (VDA 2014) 2014, 10th annual, paper 9017-21, http://vda-conference.org/program.html Differentiation of Escherichia coli serotypes using DC gradient insulator dielectrophoresis. Paul V. Jones, Alexa F. DeMichele, LaKeta Kemp, and Mark A. Hayes Anal. Bioanal. 2014, 406(1), 183-192 DOI: 10.1007/s00216-013-7437-5. article Recent developments in micro-immunoassays: varied methods and their utility in clinical testing. Christine F. Woolley and Mark A. Hayes Bioanalysis, 2013, 5(2), 245-264. article Identifying indoor environmental patterns from bioaerosol material using HPLC. Sarah J. R. Staton, Josemar A. Castillo, Thomas J. Taylor, Pierre Herckes and Mark A. Hayes* Anal. Bioanal. 2013, 405(1), 352-357. DOI: 10.1007/s00216-012-6495-4. article Cutting a drop of water pinned by wire loops using a superhydrophobic surface and knife. Ryan Yanashima, Antonio A. García, James Aldridge, Noah Weiss, Mark A. Hayes, James H. Andrews PLoS ONE, 2012, 7(9): e45893. doi:10.1371/journal.pone.0045893. article Manipulation and capture of Aß amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP), Sarah J. R. Staton, Paul V. Jones, Ginger Ku, S. Douglass Gilman, Indu Kheterpal, and Mark A. Hayes* Analyst, 2012, 137(14), 3227-3229 DOI:10.1039/C2AN35138B. article Quantitative assessment of flow and electric fields for electrophoretic focusing at a converging channel entrance with interfacial electrode. Michael Keebaugh, Prasun Mahanti, Mark Hayes, Electrophoresis, 2012, 33(13), 1924-1930. DOI: 10.1002/elps.201200199. article Exploring the feasibility of bioaerosol analysis as a novel fingerprinting technique. Josemar A. Castillo, Sarah J. R. Staton, Thomas J. Taylor, Pierre Herckes, Mark A. Hayes* Anal. Bioanal. 2012, 403(1), 15-26. DOI: 10.1007/s00216-012-5725-0, PMID: 22311424. article Using Electrophoretic Exclusion to Manipulate Small Molecules and Particles on a Microdevice. Stacy M. Kenyon, Noah G. Weiss, & Mark A. Hayes Electrophoresis, 2012, 33, 1227-1235, DOI 10.1002/elps.201100622. article Presssure-Assisted Electrokinetic Supercharging for the Enhancement of Non-Steroidal Anti-Inflammatory Drugs. Michelle M. Meighan, Mohamed Dawod, Michael C. Breadmore, Roseanne Guijt, Mark A. Hayes. Journal of Chromatography A, 2011, 1218 6750-6755, PMID: 21855878 Dielectrophoretic mobility determination in DC insulator-based dielectrophoresis. Noah G. Weiss, Paul V. Jones, Prasun Mahanti, and Mark A. Hayes* Electrophoresis, 2011, 32, 2292-2297, PMID: 21823129. article Blood cell capture in a gradient dielectrophoretic microchannel. Paul V. Jones, Sarah J. R. Staton, and Mark A. Hayes* Anal. Bioanal. 2011, 401, 2103-2111, PMID: 21830138. article