Rubinstein, John L. - University of Toronto - Department of Biochemistry

个人简介

John Rubinstein obtained his B.Sc from the University of Guelph in 1998. He received his PhD from Cambridge University (2002) where he worked in Medical Research Council laboratories under the supervision of Sir John E. Walker and Dr. Richard Henderson. Dr. Rubinstein was a Postdoctoral Research Associate at the MRC Laboratory of Molecular Biology before returning to Canada for a National Cancer Institute of Canada (NCIC) postdoctoral fellowship at the Banting and Best Department of Medical Research. He joined the Research Institute of The Hospital for Sick Children in 2006 and is a Professor in the Departments of Biochemistry and Medical Biophysics.

研究领域

Our group studies the structure and function of macromolecular assemblies using electron cryomicroscopy (cryo-EM), image analysis, molecular biology and molecular genetics. We also develop the tools of cryo-EM so that we can answer questions that are not amenable to the techniques that currently exist. This process usually occurs at the level of developing new algorithms and software for image analysis and performing calculations with images and models of molecular structure. Recent biological projects have included the structural study of the mitochondrial ATP synthase (a collaboration with the group of Sir John Walker, at the MRC Mitochondrial Biology Unit in Cambridge, UK), the Vacuolar-type ATPase, and the V/A-ATPase. We also study bacterial toxins that interact with mammalian cells.

近期论文

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Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. Anna Zhou, Alexis Rohou, Daniel G. Schep,John V. Bason, Martin G. Montgomery, John E. Walker, Niko Grigorieff, and John L. Rubinstein (2015) eLife e10180. Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase Jianhua Zhao, Samir Benlekbir, and John L. Rubinstein (2015) Nature 521, 241-5. Description and comparison of algorithms for correcting anisotropic magnification in cryo-EM images Jianhua Zhao, Marcus A. Brubaker, Samir Benlekbir, and John L. Rubinstein (2015) ArXiv 1501.05928 Alignment of cryo-EM movies of individual particles by optimization of image translations John L. Rubinstein and Marcus A. Brubaker (2014) ArXiv 1409.6789 Beyond blob-ology Martin T. J. Smith and John L. Rubinstein (2014) Science 345, 617-9. Fabrication of carbon films with ~500 nm holes for cryo-EM with a direct detector device. Chelsea R. Marr, Samir Benlekbir, and John L. Rubinstein (2013) Journal of Structural Biology 185, 42-7. Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11 Å resolution. Samir Benlekbir, Stephanie A. Bueler, and John L. Rubinstein (2012) Nature Structural and Molecular Biology 19, 1356-62. The arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM. Lindsay A. Baker, Ian N. Watt, Michael J. Runswick, John E. Walker, and John L. Rubinstein (2012) Proceedings of the National Academy of Sciences (USA) 109, 11675-80. Sub-nanometer resolution structure of the intact T. thermophilus H+-driven ATP synthase. Wilson Lau and John L. Rubinstein (2012) Nature 481, 214-8.