刘佳 - 上海科技大学 - 免疫化学研究所

个人简介

Education 2012, Ph. D in Biochemistry, University of Maryland, College Park, United States 2006, B. S. in Biological Sciences, Nankai University, P. R. China Professional 2014-present, Research Associate Professor, Shanghai Advanced Immunochemical Studies (SIAIS), ShanghaiTech University 2012-2014, postdoctoral fellow, The Scripps Research Institute, La Jolla

研究领域

Antibody-drug conjugates: plant-derived toxins, improved potency Antibody-drug conjugates (ADCs) are complex molecules that consist of monoclonal antibodies and cytotoxic drugs. It combines the unique targeting capabilities of antibodies and the efficient cancer-killing abilities of toxins. The ADC research in our lab is facilitated by the cytotoxins derived from natural products. The main feature of these compounds is the broad-spectrum cancer-killing activity. Drug lead discovery using focused compound libraries Empowered by the High-throughput Screening Platform at SIAIS, it is now possible to screen for a variety of biologically active molecules from our focused compound libraries. One of the current projects is to identify small molecules regulating cell differentiation and programming. Gene-free gene therapy: a safer solution for genetic diseases After decades of research, gene therapy is transforming from a pure concept to a practical therapeutic strategy for genetic diseases. Despite of the myriad nuclease tools such as ZFN, TALEN and CRISPR/Cas9, delivery remains a major challenge. Our lab is focused on protein-based, gene-free delivery method. Compared with other methods, protein delivery can dramatically reduce off-target effect and is widely applicable for different cell types.

近期论文

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Liu, J., Sun, Y., Zhang, X., Liang, X., Wu, Y., Wang, Y. and Jiang, X. (2014) Spirooxindoles, a potential novel class of anti-inflammatory agents. Inflamm. Cell Signal., 1, e372. Gaj, T., Liu, J., Anderson, K. E., Sirk, S. J., Barbas, C. F. III (2014) Protein delivery using Cys2-His2 zinc-finger domains. ACS Chem. Biol. 9, 1662-1667. Sun, Y., Liu, J., Sun, T., Zhang, X., Yao, J., Kai, M., Jiang, X. and Wang, R. (2014) Anti-cancer small molecule JP-8g exhibits potent in vivo anti-inflammatory activity. Sci. Rep. 14, 4:4372. Liu, J., Gaj, T., Patterson, J. T., Sirk, S. J. and Barbas, C. F., III (2014) Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering. PLoS One 9, e85755. Liu, J., Cropp, T. A. (2013) Rational protein sequence diversification by multi-codon scanning mutagenesis. in Methods in Molecular Biology (Walker, J. M. Eds.), 978, pp. 217-218, Humana Press, Totowa. Liu, J. and Cropp, T. A. (2012) Experimental methods for scanning unnatural amino acid mutagenesis, in Methods in Molecular Biology (Pollegioni, L. and Servi, S., Eds.), 794, pp. 187-197, Humana Press, Totowa. Liu, J. and Cropp, T. A. (2012) A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons. Protein Eng. Des. Sel. 25, 67-72. Castaneda, C. A., Liu, J., Chaturvedi, A., Urszula, N., Cropp, T. A. and Fushman, D. (2011) Nonenzymatic assembly of natural polyubiquitin chains of any linkage composition and isotopic labeling scheme. J. Am. Chem. Soc., 133, 17855-17868. Castaneda, C. A., Liu, J., Kashyap, T. R., Singh, R. K., Fushman, D. and Cropp, T. A. (2011) Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups. Chem. Commun. 47, 2026-2028. Liu, J., Castaneda, C. A., Wilkins, B. J., Fushman, D. and Cropp, T. A. (2010) Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids. Bioorg. Med. Chem. Lett. 20, 5613-5616. Wilkins, B. J., Marionni, S., Young, D. D., Liu, J., Wang, Y., Di Salvo, M. L., Deiters, A. and Cropp, T. A. (2010) Site-specific incorporation of fluorotyrosines into proteins in Escherichia coli by photochemical disguise. Biochemistry 49, 1557-1559. Chen, D., Liu, J., Liu, J. and Chen, X. (2010) Evolution of the catalytic activity of Arabidopsis thaliana glutathione transferase zeta class-1 by saturation mutagenesis. Biosci. Biotechnol. Biochem. 74, 1458-1461. Chen, X., Liu, J., Yang, P. and Chen, D. (2010) Identifying functional residues in Arabidopsis thaliana zeta class glutathione S-transferase through screening inactive point mutants. Biochemistry (Moscow) 75, 110-114. Tao, S., Chen, X., Liu, J., Ming, M., Chong, N. and Chen, D. (2008) Characterization of Ser73 in Arabidopsis thaliana glutathione S-transferase zeta class. J. Genet. Genomics 35, 507-512. Liu, J., Gaj, T., Wallen, M. C. and Barbas, C. F., 3rd (2014) Gene-free gene therapy by cell-penetrating zinc finger nuclease proteins. In ShanghaiTech-SIAIS Bioforum 2014-International Symposium on Cellular Processing of Information, Shanghai, P. R. China, November 17-21, 2014. Liu, J. and Cropp, T. A. (2010) Codon scanning mutagenesis by asymmetric transposons. In 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010. Liu, J. and Cropp, T. A. (2010) Facile codon deletion mutagenesis using asymmetric transposons. In 3rd Frontiers at the Chemistry and Biology Interface, College Park, MD, United States, May 1, 2010. Liu, J. and Cropp, T. A. (2009) Identification and characterization of fluorescent GFP mutants with internal deletions. In 238th ACS National Meeting, Washington, DC, United States, August 16-20, 2009.