Ananvoranich, Sirinart - University of Windsor - Department of Chemistry & Biochemistry

研究领域

Our primary interest is the study of biochemical mechanisms of the obligate intracellular parasite, Toxoplasma gondii. This protozoan parasite infects an estimated one-third of North-Americans and is probably one of the most wide-spread parasite on earth. In general, the infections in healthy adults are usually asymptomatic. However, the infections in neonates and immuno-compromised individuals can be devastating. T.gondii is indeed the causative agent of congenital toxoplasmosis, the leading cause of neurological birth defects, and toxoplasmic encephalitis the most common cause of focal brain lesions in AIDS patients. Fortunately T. gondii is an experimentally-accessible organism to which several molecular and genetic manipulation can be applied. The initial goal of the laboratory is to use antisense and ribozyme technology to disrupt the parasite nucleotide salvage pathway. The ultimate aims are to exploit these techniques to attack essential mechanisms of the parasite pathogenicity, such as invasion and differentiation.

近期论文

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Al-Anouti F, Tomavo S, Parmley S and Ananvoranich S (2004) The expression of lactate dehydrogenase is important for the cell cycle of Toxoplasma gondii Journal of Biological Chemistry 279: 52300 – 52311. Sheng J, Al-Anouti F and Ananvoranich S (2004) Engineered delta ribozymes can simultaneously knock down the expression of uracil phosphoribosyltransferase and hypoxanthine-xanthine-guanine phosphoribosyltransferase in Toxoplasma gondii. International Journal for Parasitololgy 34:253-263. Al-Anouti, F., Quach, T., and Ananvoranich S. (2003) Double-stranded RNA can mediate the suppression of uracil phosphoribosyltransferase expression in Toxoplasma gondii. Biochemical and Biophysical Research Communications 302: 316-323. Al-Anouti, F. and Ananvoranich S. (2002) Comparative analysis of antisense RNA, double-stranded RNA and delta ribozyme mediated gene regulation in Toxoplasma gondii. Antisense and Nucleic Acid Drug Development 4: 275-281. Ananvoranich, S., and Perreault J.P. (2000) The kinetics and magnesium requirements for the folding of antigenomic delta ribozymes. Biochemical and Biophysical Research Communications. 270: 600-607.