Rivera, Mario - The University of Kansas

个人简介

B.S., 1984, Universidad Autónoma de Guadalajara Ph.D., 1991, University of Arizona Postdoctoral Fellow, 1991-1994, University of Arizona>

研究领域

Bioanalytical and Bioinorganic Chemistry

Iron is an essential nutrient for most organisms, including pathogenic organisms. Pathogenic bacteria attempting to colonize (infect) an organism are confronted with extremely low concentrations of free iron. Consequently, many pathogens have evolved sophisticated mechanisms for iron acquisition, including the utilization of heme iron. Thus, it is possible that targeting paths used by pathogenic bacteria to assimilate iron and heme-iron from their host is a viable approach to the development of new antibiotics. Many of the proteins involved in heme uptake and heme utilization in the opportunistic pathogen Pseudomonas aeruginosa have designated functions. However, their structure, dynamics and inter-protein interactions needed to facilitate host-heme capture, internalization and degradation in the cytosol, are largely unknown. We aim at contributing to fill this gap by studying the structure, function, dynamics and association of the soluble proteins that aid in the capture of heme from hemoglobin and help degrade it in the cytosol of P. aeruginosa. Current efforts entail recombinant DNA methodology, NMR spectroscopy and bioelectrochemistry in this laboratory and X-ray crystallography, EPR spectroscopy and resonance Raman spectroscopy via collaborations.

近期论文

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"Concerted Motions Networking Pores and Distant Ferroxidase Centers Enable Bacterioferritin Function and Iron Traffic", Huili Yao; Huan Rui; Ritesh Kumar; Kate Eshelman; Scott Lovell, Kevin P. Battaile; Wonpil Im; and Mario Rivera, Biochemistry 2015, 54, 1611-1627. "Heme-Iron Utilization by Leptospira interrogans Requires a Heme Oxygenase and a Plastidic-Type Ferredoxin-NADP(+) Reductase", Anabel Soldano; Huili Yao; Mario Rivera; Eduardo A. Ceccarelli; Daniela L. Catalano-Dupuy, Biochimica et biophysica acta, 2014, 1840, 3208-3217. "Replacing the Axial Ligand Tyrosine 75 or its Hydrogen Bond Partner Histidine 83 Minimally Affects Hemin Acquisition by the Hemophore HasAp from Pseudomonas aeruginosa", Ritesh Kumar , Hirotoshi Matsumura , Scott W. Lovell , Huili Yao , Juan Carlos Rodriguez , Kevin P. Battaile , Pierre Moenne-Loccoz , and Mario Rivera, Biochemistry, 2014, 53, 2112-2125. "Local packing modulates diversity of iron pathways and cooperative behavior in eukaryotic and prokaryotic ferritins", Anatoly M. Ruvinsky, Ilya A. Vakser and Mario Rivera, Chem. Phys. 2014,140, 115104. "Bacterioferritin: Structure Function and Protein–Protein Interactions", Mario Rivera, Handbook of Porphyrin Science, Vol. 30, p136-179, 2014. "The Hemophore HasA from Yersinia pestis (HasAyp) Coordinates Hemin with a Single Residue, Tyr75, and with Minimal Conformational Change", Kumar, R., Slovell, S., Matsumura, H., Battaile, K., Moënne-Loccoz, P., and Rivera, M., Biochemistry, 2013, 52 (16), 2705–2707 "Heme Uptake and Metabolism in Bacteria", Benson, D.R., and Rivera, M., Met. Ions Life Sci., 2013, 12, 279-332. "Protein Dynamics and Ion Traffic in Bacterioferritin", Rui H, Rivera, M, Im W., Biochemistry, 2012, 51, 9900−9910. "Efficient and selective isotopic labeling of hemes to facilitate the study of multiheme proteins", Fonseca, B.M., Tien, M., Rivera, M., Shi, L., and Louro, R.O. Biotechniques. 2012 April, 1-7, DOI: 10.2144/000113859. "The Structure of the BfrB-Bfd Complex Reveals Protein-Protein Interactions Enabling Iron Release from Bacterioferritin", Yao H., Wang Y., Lovell S., Kumar R., Ruvinsky A.M., Battaile K.P., Vakser I.A., Rivera M., J Am Chem Soc. 2012 Aug 1. PMID: 22812654. "Accommodating a Nonconservative Internal Mutation by Water-Mediated Hydrogen Bonding between β-Sheet Strands: A Comparison of Human and Rat Type B (Mitochondrial) Cytochrome b5", Parthasarathy, S., Altuve, A., Terzyan, S., Zhang, X., Kuczera, K., Rivera, M., Benson, D., Biochemistry, 2011, 50 (24), pp 5544–5554. "Two Distinct Ferritin-like Molecules in Pseudomonas aeruginosa: The Product of the bfrA Gene Is a Bacterial Ferritin (FtnA) and Not a Bacterioferritin (Bfr)", Yao, H., Jepkorir, G., Lovell, S., Nama, P., Weeratunga, S., Battaile, K., Rivera, M., Biochemistry 2011, 14;50 (23), 5236-48. "Kinetic and Spectroscopic Studies of Hemin Acquisition in the Hemophore HasAp from Pseudomonas aeruginosa", Yukl, E.T., Jepkorir G., Alontaga A.Y., Pautsch, L., Rodríguez J.C., Rivera M., Moënne-Loccoz, P., Biochemistry 2010, 49, 6646–6654. "Structural, NMR Spectroscopic, and Computational Investigation of Hemin Loading in the Hemophore HasAp from Pseudomonas aeruginosa." Jepkorir G, Rodríguez J.C., Rui H., Im W., Lovell S., Battaile K.P., Alontaga A.Y., Yukl E.T., Moënne-Loccoz P., Rivera M., J. Am Chem Soc. 2010, June, 132(28) pp 9857-9872, PMID: 20572666. "Structural Studies of Bacterioferritin B from Pseudomonas aeruginosa Suggest a Gating Mechanism for Iron Uptake via the Ferroxidase Center", Weeratunga, S.K., Lovell, S., Yao,H., Battaile, K.P., Fischer, C.J., Gee, C., and Rivera, M., Biochemistry, 2010, 49 (6), pp 1160–1175. "Structural basis for the photoconversion of a phytochrome to the activated Pfr form", Ulijasz, A.T., Cornilescu, C.C., Zhang, J., Rivera, M., Markley, J.L., and Vierstra, R.D., Nature, 2010, January 463(7278), 250-4. "Cyanochromes Are Blue/Green Light Photoreversible Photoreceptors Defined by a Stable Double Cysteine Linkage to a Phycoviolobilin-type Chromophore", Ulijasz A.T., Cornilescu G., von Stetten D., Cornilescu C., Velazquez Escobar F., Zhang J., Stankey R.J., Rivera M., Hildebrandt P., Vierstra R.D..J Biol Chem. 2009 Oct 23;284(43):29757-72. "Binding of Pseudomonas aeruginosa Apobacterioferritin-Associated Ferredoxin to Bacterioferritin B Promotes Heme Mediation of Electron Delivery and Mobilization of Core Mineral Iron", Weeratunga, S.K., Gee, C.E., Lovell, S., Zeng, Y., Woodin, C.L. and Rivera, M., Biochemistry 2009, 48, 7420–7431. "Structural Characterization of the Hemophore HasAp from Pseudomonas aeruginosa: NMR Spectroscopy Reveals Protein-Protein Interactions between Holo-HasAp and Hemoglobin", Alontaga, A., Rodriguez, J., Schönbrunn, E., Becker, A., Funke, T., Yukl, E., Hayashi, T., Stobaugh, J., Moënne-Loccoz, P., and Rivera, M., Biochemistry, 2009, 48 (1), 96-109. "The Dual Role of Heme as Cofactor and Substrate in the Biosynthesis of Carbon Monoxide", Rivera, M. and Rodriguez, J.C., Met. Ions Life Sci. 2009, 6, 241-293