个人简介

ducation B.S. Chemistry, Seoul National University, Korea, 1987 M.S. Chemistry, Seoul National University, Korea, 1989 Ph.D. Biochemistry, University of Wisconsin-Madison, USA, 1994 Research Experiences Research Associate, Howard Hughes Medical Institute/Massachusetts Institute of Technology, 1994-1997 Principal Investigator, Samsung Biomedical Research Institute, 1997-1999 CEO and CSO, ToolGen, Inc., 1999-2005 Director, Center for Genome Engineering, Institute for Basic Science, 2014-present

研究领域

BIO

Current Research Interests We focus on developing programmable nucleases that enable genome editing in plants, animals, and cultured cells including human pluripotent stem cells. These nucleases cleave chromosomal DNA in a targeted manner, producing DNA double-strand breaks (DSBs), whose repair via endogenous mechanisms gives rise to targeted genome modification in cells and whole organisms. For the last ten years or so, we have developed three different types of programmable nucleases, namely, zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) derived from the type II CRISPR/Cas prokaryotic adaptive immune system. These nucleases are now used widely in almost every discipline in biology, biotechnology, and molecular medicine.

近期论文

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Kim, D., Bae, S., Park, J., Kim, E., Kim, S., Yu, H. R., Hwang, J., Kim, J.-I., Kim, J.-S. (2015) Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nature Methods. Advenced Online published Bae, S., Kweon, J., Kim, H. S., Kim, J.-S. (2014) Microhomology-based choice of Cas9 nuclease target sites. Nature Methods. 11, 705-706 Park, C.-Y., Kim, J., Kweon, J., Son, J. S., Lee, J. S., Yoo, J.-E., Cho, S.-R., Kim, J.-H., Kim J.-S., Kim D.-W. (2014) Targeted inversion and reversion of the blood coagulation factor 8 gene in human iPS cells using TALENs. PNAS Lee, J.-S., Kwak, S.-J., Kim, J., Noh, H. M., Kim, J.-S., Yu, K. (2014) RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein. G3 Moon, J., Lee, C., Kim, S. J., Choi, J.-Y., Lee, B. C., Kim, J.-S., Jang, G. (2014) Production of CMAH Knockout Preimplantation Embryos Derived From Immortalized Porcine Cells Via TALE Nucleases. Molecular Therapy Nucleic Acids. 3, 166 Kim, S., Kim, D., Cho, S.-W., Kim, J., Kim J.-S. (2014) Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Research. 24, 1012-1019 Kim, H., and Kim J.-S. (2014) A guide to genome engineering with programmable nucleases. Nature Reviews Genetics, 15, 321–334 Ramakrishna, S., Cho, S.-W., Kim, S., Song, M., Gopalappa, R., Kim J.-S. and Kim, H. (2014) Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations. Nature Communications. 5, 3378 Bae, S., Park, J., Kim J.-S. (2014) Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics, 30 (10): 1473-1475 Kim, J. M., Kim, D., Kim, S., Kim J.-S. (2014) Genotyping with CRISPR-Cas-derived RNA-guided endonucleases. Nature Communications, 5, 3157 Sung, Y. H., Kim, J. M., Kim, H.-T., Lee, J., Jeon, J., Jin, Y., Choi, J.-H., Ban, Y. H., Ha, S.-J., Kim, C.-H., Lee, H.-W., Kim J.-S. (2013) Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases. Genome research. 24, 125-131 Cho, S. W., Kim, S., Kim, Y., Kweon, J., Kim, H. S., Bae, S., Kim, J.-S. (2013) Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome research. 24, 132-141 Lee, S.-W., Kim, E., Kim, J.-S., Oha, M.-K. (2013) Artificial transcription regulator as a tool for improvement of cellular property in Saccharomyces cerevisiae. Chemical Engineering Science, 103, 42-49 Kim Y.-K., Wee G., Park J., Kim J., Baek D., Kim J.-S., Kim V. N. (2013) TALEN-based knockout library for human microRNAs. Nature Structural & Molecular Biology. 20, 1458–1464 Cho S.-W., Lee J, Carroll D., Lee J., Kim J.-S. (2013) Heritable gene knockout in C. elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics. 195, 1177-1180 Kim H., Kim M.-S., Wee G., Lee C.-I., Kim H., Kim J.-S. (2013) Magnetic Separation and Antibiotics Selection Enable Enrichment of Cells with ZFN/TALEN-Induced Mutations. PLoS One. 8 Kim Y., Kweon J., Kim A., Chon J.-K., Yoo J.-Y., Kim H.-J., Kim S., Lee C., Jeong E., Chung E., Kim D., Lee M.-S., Go E.-M., Song H.-J., Kim H., Cho N., Bang D., Kim S., Kim J.-S. (2013) A library of TAL effector nucleases spanning the human genome. Nat Biotechnol. 31, 251-258