个人简介

Education B.S. Food Science and Biotechnology, Seoul National University, Korea, 2000 M.S. Chemistry, Seoul National University, Korea, 2002 Ph.D. Chemistry, Seoul National University, Korea, 2007 Research Experiences Assistant Professor, Department of Bio & Nano Chemistry, Kookmin University, 2010-2014 Postdoctoral Fellow, NIDDK, National Institutes of Health, USA, 2007-2010 Postdoctoral Researcher, The Research Institute of Basic Sciences, Seoul National University, 2007-2007

研究领域

BIO

Current Research Interests The protein-protein (or membrane lipid) interplays are central to almost every aspect of cell biology. We are interested in fundamental questions of how the interactions between proteins and proteins (or membranes) determine cell and organelle shape, how protein-protein or protein-lipid interactions turn on and off the signals that control essential cell processes including cytokinesis, which is the final stage of cell division. We are also highly interested in determining atomic structures of membrane proteins. It is easy to agree that "Seeing is believing". The roots of our research are in the power of x-ray crystallography to reveal the structures of biomolecules at atomic level, leading to insights in the physical basis of protein-protein interactions. The core approach is complemented by studies in solution or on membranes by hydrodynamic, small angle x-ray scattering, fluorescence imaging, and electron microscopy. The structural, biochemical, and biophysical experiments lead to new hypotheses about biological function and mechanism. We also try to demonstrate our new hypotheses in cells using various cell biological tools. Crystal structures of drug target proteins can also be used to discover new lead compounds by structure-based drug discovery (SBDD) method. We are interested in various drug target proteins to treat various diseases including cancer.

近期论文

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Son SH and Lee HH (2015), "Crystallization and preliminary X-ray crystallographic analysis of Z-ring associated protein (ZapD) from Escherichia coli", Acta Crystallogr. F71:194-198. Choe H, Ha JM, Joo JC, Kim H, Yoon H-J, Kim S, Son SH, Gengan RM, Jeon ST, Chang R, Jung KD, Kim YH* and Lee HH* (2015), “Structural insights into efficient CO2-reducing activity of an NAD-dependent formate dehydrogenase from Thiobacillus sp. KNK65MA”, Acta Crystallogr. D71 Epub (doi:10.1107/S1399004714025474) (*co-corresponding author) Kim S-K*, Park Y-C*, Lee HH*, Jeon ST, Min W-K, Seo J-H (2015), "Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli", Biotechnol Bioeng. 112(2):346-355. Lee WK, Son SH, Jin BS, Na JH, Kim SY, Eunice EK Kim, Yu YG* and Lee HH* (2013), "Structural and functional insights into the regulation mechanism of CK2 by IP6 and the intrinsically disordered protein Nopp140", Proc. NatI. Acad. Sci. USA 110(48):19360-19365. (*co-corresponding author) Matsuura A and Lee HH (2013), "Crystal structure of GTPase-activating domain from human MgcRacGAP", Biochem. Biophys. Res. Commun. 435(3):367-372. Son SH and Lee HH (2013), "The N-terminal domain of EzrA binds to the C terminus of FtsZ to inhibit Staphylococcus aureus FtsZ polymerization“, Biochem. Biophys. Res. Commun. 433(1):108-114. Han SG, Lee WK, Jin BS, Lee KI, Lee HH, and Yu YG* (2013), "Identification of Novel Irreversible Inhibitors of UDP-N-Acetylglucosamine Enolpyruvyl Transferase (MurA) from Haemophilus influenzae“, J. Microbiol. Biotechnol. 23(3):329-334. Lee HH* and Jung ST (2013), "Overexpression, crystallization, and preliminary X-ray crystallographic analysis of β-N-acetylglucosaminidase from Thermotoga maritima by the Tm0809 gene" Acta Crystallogr. F69(Pt 2):115-117. (*corresponding author) Matsuura A*, Yoon JY*, Yoon HJ*, Lee HH**, Suh SW** (2012), "Crystal structure of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii“, Mol Cells 34(4):407-412. (*equally contributed, **co-corresponding author) Son SH, Lee DY, Kim YJ, Ko S, Cho SJ, Jung HC, Lee HH (2012), "Cloning, expression, and polymerization assay of FtsZ protein from Staphylococcus aureus“, KJMB 40(3):274-277. Lee SJ, Lee S, Lee SK, Yoon H, Lee HH, Kim KK, Lee BJ, Lee BI, and Suh SW* (2012), "Crystal structures of the Staphylococcus aureus peptide deformylase in complex with two classes of new inhibitors“, Acta Crystallogr. Sec D 68(Pt 7):784-793. Yoon JY, Park CR, Lee HH* and Suh SW* (2012), "Overexpression, crystallization, and preliminary X-ray crystallographic analysis of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii“, Acta Crystallogr. 68(Pt 4):440-442. (*co-corresponding author) Lee HH*, Nemecek D*, Schindler C*, Smith WJ, Ghirlando R, Steven AC, Bonifacino JS, and Hurley JH (2012), "Assembly and Architecture of the Biogenesis of Lysosome-Related Organelles Complex-1 (BLOC-1)“, J. Biol. Chem. 287(8):5882-5890. (*equally contributed) Lee HH (2012), "High-resolution structure of shikimate dehydrogenase from Thermotoga maritima reveals a novel tightly closed conformation.“, Mol Cells 33(3):229-233. Lee HH (2011), "Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Archaeoglobus fulgidus“, Acta Crystallogr. 67(Pt 12):1556-1558. Yoon HJ, Kang JY. Mikami B, Lee HH*, Suh SW* (2011), “Crystal structure of phosphopantetheine adenylyltransferase from Enterococcus faecalis in the ligand-unbound state and in complex with ATP and pantetheine”, Mol Cells 32(5):431-435. (*co-corresponding author) Lee HH (2011), “Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Thermotoga maritima”, Acta Crystallogr. 67(Pt 7):824-826. Lee HH*, Jang JY, Yoon HJ, Kim SJ, Suh SW* (2010), “Crystal structures of two archaeal Pelotas reveal inter-domain structural plasticity”, Biochem. Biophy. Res. Commun. 399(4):600-606. (*co-corresponding author) Lee HH*, Suh SW* (2010), “Overexpression, crystallization, and preliminary X-ray crystallographic analysis of Pseudomonas aeruginosa MnmE, a GTPase involved in tRNA modification”, Acta Crystallogr. 66(Pt 8):905-908. (*co-corresponding author) Lee HH*, Yoon HJ*, Kang JY, Park JH, Kim DJ, Choi KH, Lee SK, Son J, Kim HJ, Suh SW (2009), “Crystal structure of Staphylococcus aureus phosphopantetheine adenylyltransferase in complex with 3’ -phosphoadenosine 5’-phosphosulfate reveals a new ligand binding mode”, Acta Crystallogr. F65: 987-991. (International Union of Crystallography) (*equally contributed)