Craig, Douglas B. - University of Manitoba

个人简介

B.Sc. (1988) Mount Allison Ph.D. (1993) Dalhousie Post-Doc: Manitoba (1993-1994), Alberta (1994-1997)

研究领域

Chemical studies typically involve measurements of large ensembles of molecules with data representing averages. With respect to enzymes, we now know such average values represent a simplification. Single enzyme molecule assay utilizing ultrasensivitive capillary electrophoresis laser-induced fluorescence instrumentation have demonstrated that individual enzyme molecules are not identical. Individual molecules differ with respect to rate and activation energy of catalysis. My research interests are in the study of the basis and function of the heterogeneity of enzyme molecules and its role within the cell. A second interest is the development of ultrasensitive methods for the analysis of biological molecules, particulary enzymes and proteins.

近期论文

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Craig, DB, Hanlon-Dearman, F, Beaudry, S, Shek, K & King, SD (2015) Comparison of the single molecule activity distributions of recombinant and non-recombinant bovine intestinal alkaline phosphatase. Protein Expression & Purification 114, 95-98. Craig, DB, Reinfelds, G & Henderson, A (2014) 12-Channel Peltier array temperature control unit for single molecule enzymology studies using capillary electrophoresis. Electrophoresis 35, 2408-2411. Ahmadi, S, Absalan, G, Craig, DB & Goltz, DM (2014) Photochemical properties of purpurin and its implications for capillary electrophoresis with laser induced fluorescence. Dyes and Pigments 105, 57-62. Ahmadi, S, Craig, DB & Goltz, DM (2013) Micellar electrokinetic chromatography with laser-induced fluorescence detection for separation of red and yellow historical dyes. Chromatography 1, 9-23. Craig, DB (2013) Single enzyme molecule assay with time resolution using capillary electrophoresis. Rev. Anal. Chem. 32, 103-112. Craig, DB & Henderson, A (2013) Electrophoretic heterogeneity limits the utility of streptavidin-beta-galactosidase as a probe in free zone capillary electrophoresis separations. Protein J. 32, 81-88. Craig, DB, Bayaraa, B, Lee, D & Charleton, J (2013) Effect of induction temperature and partial thermal denaturation on the catalytic and electrophoretic heterogeneity of beta-galactosidase from two Escherichia coli strains. J. Liq. Chromatogr. R. T. 36, 2944-2959. Craig, DB, Morris, TT & Ong-Justiniano, CMQ (2012) Measurement of the activity of individual subunits of single molecules of the tetrameric enzyme beta-galactosidase. Anal. Chem. 84, 4598-4602. Craig, DB & Chase, LN (2012) Arrhenius plot for a reaction catalyzed by a single molecule of beta-galactosidase. Anal. Chem. 84, 2044-2047. Craig, DB, Schwab, T & Sterner, R (2012) Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of beta-galactosidase. Biochem. Cell. Biol. 90, 540-547. Craig, DB (2012) A simple system for the measurement of the distribution of activities of individual molecules of E. coli beta-galactosidase. Anal. Methods 4, 85-88. Goltz, DM, Ahmadi, S, Absalan, G & Craig, DB (2012) Separation of historical dyes using capillary electrophoresis with laser-induced fluorescence detection. J. Liq. Chromatogr. R. T. 35, 2054-2065. Craig, DB & Nichols, ER "Sample preparation for single molecule enzymology using capillary electrophoresis with fluorescence detection" in "Comprehensive sampling and sample preparation", volume 3; J Pawliszyn, XC Le, XF Li, HK Lee Eds; Elsevier, Academic Press: Oxford UK, pp 323-337 (2012). Craig, DB, Eggertson, MJ, Chikamatsu, M & Horwood, CA (2011) Single molecule assay of Escherichia coli beta-galactosidase using two competing substrates simultaneously; DDAO-beta-D-galactoside and resorufin-beta-D-galactoside. Anal. Lett. 44, 1835-1841. Craig, DB, Haslam, AM, Silverstein, HJ, Chikamatsu, M, Shadabi, E & Nichols, ER (2010) Effect of size, quaternary struture and translational error on the static and dynamic heterogeneity of beta-galactosidase and measurement of the electrophoretic dynamic heterogeneity. Protein J. 29, 398-406. Craig, DB (2010) Heterogeneous properties of individual molecules of beta-galactosisdase from the thermophilic bacteria Geobacillus stearothermophilus. Protein J. 29, 55-61. Craig, DB, Haslam, AM, Coombs, JML & Nichols, ER (2010) Kinetic studies on unmodified individual Escherichia coli beta-galactosidase molecule in free solution. Biochem. Cell Biol. 88, 451-458. Nichols, ER, Shadabi, E & Craig, DB (2009) Effect of alteration of translation error rate on enzyme microheterogeneity as assessed by variation in single molecule electrophoretic mobility and catalytic activity. Biochem. Cell Biol. 87, 517-529. Craig, DB & Nichols, ER (2008) Continuous flow assay for the simultaneous measurement of the electrophoretic mobility, catalytic activity and its variation over time of individual molecules of Escherichia coli beta-galactosidase. Electrophoresis 29, 4298-4303. Nichols, ER & Craig, DB (2008) Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microhetermgeneity. Electrophoresis 29, 4257-4269. Nichols, ER & Craig, DB (2008) Single molecule assays reveal differences between in vivo and in vitro synthesized beta-galactosidase. Protein J. 27, 376-383. Nichols, ER, Gavina, JMA, McLeod, RG & Craig, DB (2007) Single molecule assays of beta-galactosidase from two wild-type strains of E. coli: Effects of protease inhibitors on microheterogeneity and different relative activities with differing substrates. Protein J. 26, 95-105. Craig, DB, Ash, HL & Nachtigall, JT (2006) Sphingomonas paucimobilis is capable of modifying polycyclic aromatic compounds present at sub-nanomolar concentrations in a complex medium. J. Environ. Biol. 27, 305-308. Craig, DB & Nichols, ER (2006) Spectroscopic measurement of the redox potential of cytochrome c for the undergraduate biochemistry laboratory. J. Chem. Ed. 83, 1325-1326. Craig, DB, Wetzl, BK, Duerkop, A & Wolfbeis, OS (2005) Determination of picomolar concentrations of protein using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection. Electrophoresis 26, 2208-2213.