Yao, Xiao-Jian - University of Manitoba - Department of Medical Microbiology and Infectious Diseases

研究领域

Investigation of molecular mechanisms underlying the early stage of HIV-1 replication, especially on HIV-1 integrase/cellular protein interactions required for HIV-1 DNA nuclear import, chromatin targeting and integration. Study on the mechanism involved in the combat between HIV-1 Vif and host defenders during HIV-1 replication, and development of new antiviral strategies by using host anti-HIV molecules. Development of new preventative and therapeutic strategies against HIV-1 infection and transmission. Development of a safe and sensitive avian influenza virus entry system for studying of the impact of H5N1 HA mutations on virus entry in human cells and for screening antiviral agents.

近期论文

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Ao Z-j, X-J Yao* and E.A. Cohen*. Assessment of the Role of the Central DNA Flap in Human Immunodeficiency Virus Type 1 Replication using a Single-Cycle Replication System. J. Virol. 2004, 78:3170-3177. (* corresponding authors) Yao X-J, N. Rougeau, G. Duisit, J. Lemay and é.A. Cohen. Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae. Retrovirology. 2004, 1(12): 1-11. Ao Z-j, K.R. Fowke, éric A. Cohen and X-j Yao*. Contribution of the C-terminal tri-lysine regions of HIV-1 integrase for efficient reverse transcription and viral DNA nuclear import. Retrovirology 2005, 2(62): 1-15. Ao Z-j, G-y Huang, H. Yao, Z-k Xu, M. Labine, A. Cochrane, X-j Yao*. Interaction of human immunodeficiency virus type 1 integrase with cellular nuclear import receptor importin 7 and its impact on viral replication. J Biol Chem. 2007, 282:13456-67. Ao Z-j, Z Yu, L Wang, Y-f Zheng, and X-J Yao*. Vpr14-88-Apobec3G fusion protein is efficiently incorporated into Vif-positive HIV-1 particles and inhibits viral replication. PLoS ONE 2008. 16;3(4):e1995 Liang B.B, M. Luo, T.B. Ball, X-j Yao, G Wilfred R. Cuff, Domselaar, M. Cheang, S. Jones, and F.A. Plummer. Systematic analysis of host immunological pressure on the envelope gene of human immunodeficiency virus type 1 by an immunobioinformatics approach. Curr. HIV Res. 2008.(4):370-9. Ao Z-j, A. Patel, K. Tran, X-y He, K. Fowke, K. Coombs, D. Kobasa, G. Kobinger, and X-j. Yao*. Characterization of a trypsin-dependent avian influenza H5N1 hemagglutinin pseudotyped HIV vector system and Screening for inhibitory peptides. Antiviral Research 2008. 79:12-18. Xu Z-k, Y-F Zheng, Z-j Ao, M. Clement, A. Mouland, G.V Kalpana, .P Belhumeur, E.A. Cohen, and X-j Yao*. Requirement of the chromatin binding region within the C-terminal catalytic core domain of HIV-1 integrase for the yeast lethality and viral replication. Retrovirology. 2008. 5(102)p1-15 Oyugi J., F.C.M. Vouriot, J. Alimonti, S. Wayne, M. Luo, A. Land, Z-j AO, X-j Yao, R.P. Sekaly, L.J. Elliott, J.N Simonsen, T..B Ball, W. Jaoko, J. Kimani, F.A. Plummer and K.R. Fowke. A common CD4 gene variant is associated with a greater risk of HIV-1 infection in Kenyan female sex workers. J. Infect. Dis. 2009. 199(9):1327-34. Patel A, Tran K, Gray M, Li Y, Ao Z, Yao X-j, Kobasa D, Kobinger GP. Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses. Vaccine. 2009 27(23):3083-9 Lehmann, MG, Abrahamyan LG, Milev MP, X-J Yao, Pante N, and Mouland AJ. Intracellular Transport of HIV-1 genomic RNA is Dependent on Dynein and Late Endosomal Vesicles. J. Biol. Chem. 2009, 284(21):14572-85. Awah FM, P.N. Uzoegwu, J.O. Oyugi b, J. Rutherford b, P. Ifeonu a, X-J Yao, K. R. Fowke, M.O. Eze. Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract. Food Chemistry. 2010, 119:1409–1416. Ji H-z, T.B. Ball, Z-j Ao, J. Kimani, X-j Yao, and F. Plummer. Reduced HIV-1 LTR transcription in subjects with protective IRF-1 polymorphisms: A potential mechanism mediating resistance to infection by HIV-1. Scandinavian Journal of Infectious Diseases. 2010.42:389-94. He, M-z, N. Yang, X-J Yao, C-l Sun and M. Yang. Modification and biological evaluation of novel 4-hydroxy-pyrone derivatives as non-peptidic HIV-1 protease inhibitors. Medicinal Chemistry Research. 2010. (Epub Feb 02) Awah F., P. Uzoegwu, P. Ifeonu, J. Rutherford, X-j. Yao, F. Fehrmann, K. Fowke, A. Tsopmo, J. Oyugi, and M. Eze. Superoxide Anion Production in Phytohemagglutinin A and Staphylococcal Enterotoxin B-activated Human Peripheral Blood Mononuclear Cells. Nigerian Journal of Biochemistry and Molecular Biology. 2010, 25:18-24. Ao,Z-j, K.Danappa Jayappa, Y-f Zheng, G. Kobinger, and X-j Yao*. Characterization of anti-HIV activity mediated by HIV-1 integrase C-terminal domain polypeptide expressed in susceptible cells. Journal of Antivirals and Antiretrovirals. 2010, 2:20-28. Y-f. Zheng, Z-j. Ao, K. Danappa Jayappa, and X-j Yao*. Characterization of HIV-1 Integrase’s Host Chromatin and LEDGF/p75 Binding Affinity by Mutagenesis Within Catalytic Core Domain of Integrase. Virology Journal. 2010, 7:68. He, M-z, H. Zhang, X-j Yao, M. Eckart, E Zuo and M. Yan. Design, Biological Evaluation and SAR of Novel Pseudo-peptide Incorporating Benzheterocycles as HIV-1 Protease Inhibitors. Chemical Biology & Drug Design.2010, 76:174–180. Ao, Z-J., K. Danappa Jayappa, B-C Wang, Y-F Zheng, S. Kung, E. Rassart, R. Depping, M. Kohler, EA. Cohen and X-j Yao*. Importin a3 interacts with HIV-1 integrase and is required for HIV-1 nuclear import and replication. Journal of Virology. 2010, 84:8650-8663. Ao Z-j, X-X Wang, A. Bello, K. Danappa Jayappa, Z. Yu, X-Y He, K. Fowke, Xi Chen, J-h Li, G. Kobinger, and X-j Yao* Characterization of the anti-HIV activity mediated by various R88-Apobec3G mutant fusion proteins in CD4+ T cells, PBMC and macrophages. Human Gene Therapy.2011,. Y-f Zheng, Z-j Ao, B- Wang, K Danappa Jayappa, and X-j Yao*. Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication. Journal of Biological Chemistry. 2011, 286:17722–17735. Awah F.M., P.N. Uzoegwu, P. Ifeonu, J.O. Oyugi, J. Rutherford, X-j Yao, F. Fehrmann, K.R. Fowke, and M.O. Eze, Free radical scavenging activity, phenolic contents and cytotoxicity of selected Nigerian medicinal plants. Food Chemistry, 2011, 131:1279-1286. Danappa Jayappa, K., Z-j Ao, M. Yang, J-z Wang, and X-j Yao*. Identification of critical motifs in HIV-1 integrase required for Importin a3 interaction and its involvement in viral cDNA nuclear import. Journal Molecular. Biology. 2011, 410:847-862. Patel A, Tran K, Gray M, Li Y, Yao X-j, Kobinger GP. Co-administration of certain DNA vaccine combinations expressing different H5N1 influenza virus antigens can be beneficial or detrimental to immune protection. Vaccine. 2011, 30:626-36. Ao Z-j., K. Danappa Jayappa, B-c Wang, Y-f Zheng, X-x Wang, J-y Peng, and X-j Yao*, Contribution of Host Nucleoporin 62 in HIV-1 Integrase Chromatin Association and Viral DNA Integration. Journal of Biological Chemistry. 2012. 287:10544-55. Danappa Jayappa, K, AZ-j Ao, X-j Yao*, The HIV-1 passage from cytoplasm to nucleus: the process involving a complex exchange between the components of HIV-1 and cellular machinery to access nucleus and successful integration. International Journal of Biochemistry and Molecular Biology. 2012, 3(1):105-116.