个人简介

B.Sc., University of British Columbia Ph.D., University of Cambridge

研究领域

Signalling in Drosophila

The development of a multicellular organism is an incredibly complex process, and we focus on two aspects, tissue morphogenesis and formation of the nervous system. The morphogenesis of epithelial tissues sculpts an organism into its final form, and we study epithelial morphogenesis in the ovary and the embryo. This work may aid in understanding common birth defects, as well as provide clues as to why so many cancers are derived from epithelial cells. We are also interested in the formation and regulation of synapses at the neuromuscular junction, work that may provide insights into the causes of neurodegenerative diseases.

近期论文

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Harden, N., Wang, SJ. and Krieger, C. (2016). Making the connection-shared molecular machinery and evolutionary links underlie the formation and plasticity of occluding junctions and synapses. J. Cell Sci. 129: 3067-3076. Abstract Krieger, C., Wang SJ, Yoo, SH and Harden, N. (2016). Adducin at the neuromuscular junction in amyotrophic lateral sclerosis: hanging on for dear life. Front. Cell. Neurosci. Paper Wang, S., Yoo, S., Kim, H.y., Wang, M., Zheng, C., Parkhouse, W., Krieger, C., Harden, N. (2015). Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay. J. Vis. Exp. Paper Vlachos, S., Jangam, S., Conder, R., Chou, M., Nystul, T. and Harden, N. (2015) A Pak-regulated cell intercalation event leading to a novel radial cell polarity is involved in positioning of the follicle stem cell niche in the Drosophila ovary. Development 142: 82-91. Abstract Wang SJ, Tsai A, Wang M, Yoo S, Kim HY, Yoo B, Chui V, Kisiel M, Stewart B, Parkhouse W, Harden N, and Krieger C. (2014) Phospho-regulated Drosophila adducin is a determinant of synaptic plasticity in a complex with Dlg and PIP2 at the larval neuromuscular junction. Biol. Open 3: 1196-1206. full text Shen, W., Chen, X., Cormier, O., Cheng, D., Reed, B. and Harden, N. (2013) Modulation of morphogenesis by Egfr during dorsal closure in Drosophila. PLoS ONE 8(4): e60180. full text Wang, S., Yang, J., Tsai, A., Kuca, T., Sanny, J., Lee, J., Dong, K., Harden, N. and Krieger, C. (2011) Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane. Dev. Biol. 357: 392-403. Abstract Vlachos, S., and Harden, N. (2011). Genetic evidence for antagonism between Pak protein kinase and Rho1 small GTPase signaling in regulation of the actin cytoskeleton during Drosophila oogenesis. Genetics 187: 501-512. Abstract Bahri, S., Wang, S., Conder, R., Choy, J., Vlachos, S., Dong, K., Merino, C., Sigrist, S., Molnar, C., Yang, X., Manser, E., and Harden, N. (2010) The leading edge during dorsal closure as a model for epithelial plasticity: Pak is required for recruitment of the Scribble complex and septate junction formation. Development 137:2023-2032. Abstract Fricke, R., Gohl, C., Dharmalingam, E., Grevelhörster, A., Zahedi, B., Harden, N., Kessels, M., Qualmann, B., and Bogdan, S. (2009) Drosophila Cip4/Toca-1 integrates membrane trafficking and actin dynamics through WASP and SCAR/WAVE. Curr. Biol. 19:1429-1473. Abstract Zahedi, B., Shen, W., Xu, X., Chen, X., Mahey, M., and Harden, N. (2008) Leading edge-secreted Dpp cooperates with ACK-dependent signaling from the amnioserosa to regulate myosin levels during dorsal closure. Dev. Dyn. 237:2936-2946. Abstract Conder, R., Yu, H., Zahedi, B., and Harden, N. (2007) The serine/threonine kinase dPak is required for polarized assembly of F-actin bundles and apical-basal polarity in the Drosophila follicular epithelium. Dev. Biol. 305:470-482. Abstract Sanny, J., Chui, V., Langmann, C., Pereira, C., Zahedi, B., and Harden, N. (2006) Drosophila RhoGAP68F is a putative GTPase activating protein for RhoA participating in gastrulation. Dev. Genes Evol. 216:543-550. Abstract Tsuda, M., Langmann, C., Harden, N., and Aigaki, T. (2005). The RING-finger scaffold protein Plenty of SH3s targets TAK1 to control immunity signalling in Drosophila. EMBO Rep 6:1082-1087. Abstract Harden, N. (2005) Of grainy heads and broken skins. Science 308:364-365. Abstract Conder, R., Yu, H., Ricos, M., Hing, H., Chia, W., Lim, L., and Harden, N. (2004) dPak is required for integrity of the leading edge cytoskeleton during Drosophila dorsal closure but does not signal through the JNK cascade. Dev. Biol. 276: 378-390. Abstract Wei, H.-C., Sanny, J., Shu, H., Baillie, D. L. , Brill, J. A., Price, J. V. and Harden, N. (2003). The Sac1 lipid phosphatase regulates cell shape change and the JNK cascade during dorsal closure in Drosophila. Curr. Biol. 13: 1882-1887. Abstract Schenck, A., Bardoni, B., Langmann, C., Harden, N., Mandel, J. L. and Giangrande, A. (2003). CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the Fragile X protein. Neuron 38: 887-898. Abstract Harden, N. (2002). Signaling pathways directing the movement and fusion of epithelial sheets: lessons from dorsal closure in Drosophila. Differentiation 70:181-203. Abstract Sem, K.P., Zahedi, B., Tan, I., Deak, M., Lim, L., and Harden, N. (2002). ACK family tyrosine kinase activity is a component of Dcdc42 signaling during dorsal closure in Drosophila melanogaster. Mol. Cell. Biol. 22:3685-97. Abstract Harden, N., Ricos, M., Yee, K., Sanny, J., Langmann, C., Yu, H., Chia, W., and Lim, L. (2002). Drac1 and Crumbs participate in amnioserosa morphogenesis during dorsal closure in Drosophila. J Cell Sci. 115:2119-29. Abstract Technical Publication. Harden, N. and Lim, L. Bio-Rad Application Note MRC-A27. Characterization of developmental regulation of the cytoskeleton using confocal microscopy. Hing, H., Xiao, J., Harden, N., Lim, L., and S. L. Zipursky. (1999). Pak functions downstream of Dock to regulate photoreceptor axon guidance in Drosophila. Cell 97: 853-863. Abstract Ricos, M., Harden, N., Sem, K. P., Lim, L. and Chia, W. (1999). Dcdc42 acts in TGF-beta signaling during Drosophila morphogenesis: distinct roles for the Drac1/JNK and Dcdc42/TGF-beta cascades in cytoskeletal regulation. J. Cell Sci. 112: 1225-1235. Abstract Harden, N., Ricos, M., Ong, Y. M., Chia, W. and Lim, L. (1999). Participation of small GTPases in dorsal closure of the Drosophila embryo: distinct roles for Rho subfamily proteins in epithelial morphogenesis. J. Cell Sci. 112: 273-284. Abstract