GILCH, SABINE - University of Calgary - Department of Biochemistry & Molecular Biology

个人简介

B.Sc. (Bachelor of Science) Ph.D. (Doctor of Philosophy) M.Sc. (Master of Science)

研究领域

We use cell culture and animal models to study the molecular and cellular biology of prion infections. Prions are unique pathogens that consist solely of a misfolded isoform of the cellular prion protein PrPc, denominated PrPSc. Prominent examples for prion diseases are Creutzfeldt-Jacob disease (CJD) in humans, bovine spongiform encephalopathy (BSE) in cows and chronic wasting disease (CWD) in cervids. Our research program addresses the question how prion infection interferes with neuronal metabolism, and how this may lead to neurodegeneration. Furthermore, we use peptide aptamers for interference with prion propagation and for characterization of the PrPc-PrPSc binding interface. Another focus of research is CWD and aims to understand the molecular basis of the unusual peripheral distribution and shedding of CWD prions, e.g. in saliva, urine or feces. The long-term goal of our research is to translate basic research into novel diagnostic or therapeutic targets to combat prion diseases.

近期论文

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Early and Non-Invasive Detection of Chronic Wasting Disease Prions in Elk Feces by Real-Time Quaking Induced Conversion.Cheng YC, Hannaoui S, John TR, Dudas S, Czub S, Gilch S.PLoS One. 2016 Nov 9;11(11):e0166187. doi: 10.1371/journal.pone.0166187. Prion infection impairs lysosomal degradation capacity by interfering with rab7 membrane attachment in neuronal cells.Shim SY, Karri S, Law S, Schatzl HM, Gilch S.Sci Rep. 2016 Feb 11;6:21658. doi: 10.1038/srep21658. Oxidative Decarboxylation of Short-Chain Fatty Acids to 1-Alkenes.Dennig A, Kuhn M, Tassoti S, Thiessenhusen A, Gilch S, Bülter T, Haas T, Hall M, Faber K.Angew Chem Int Ed Engl. 2015 Jul 20;54(30):8819-22. doi: 10.1002/anie.201502925. Cholesterol balance in prion diseases and Alzheimer's disease.Hannaoui S, Shim SY, Cheng YC, Corda E, Gilch S.Viruses. 2014 Nov 20;6(11):4505-35. doi: 10.3390/v6114505. Review. Assessing proteinase K resistance of fish prion proteins in a scrapie-infected mouse neuroblastoma cell line.Salta E, Kanata E, Ouzounis CA, Gilch S, Schätzl H, Sklaviadis T.Viruses. 2014 Nov 13;6(11):4398-421. doi: 10.3390/v6114398. Early detection of chronic wasting disease prions in urine of pre-symptomatic deer by real-time quaking-induced conversion assay.John TR, Schätzl HM, Gilch S.Prion. 2013 May-Jun;7(3):253-8. doi: 10.4161/pri.24430. A genome-wide survey for prion-regulated miRNAs associated with cholesterol homeostasis.Montag J, Brameier M, Schmädicke AC, Gilch S, Schätzl HM, Motzkus D.BMC Genomics. 2012 Sep 17;13:486. doi: 10.1186/1471-2164-13-486. Failure of prion protein oxidative folding guides the formation of toxic transmembrane forms.Lisa S, Domingo B, Martínez J, Gilch S, Llopis JF, Schätzl HM, Gasset M.J Biol Chem. 2012 Oct 26;287(44):36693-701. doi: 10.1074/jbc.M112.398776. Severe acute respiratory syndrome coronavirus replication is severely impaired by MG132 due to proteasome-independent inhibition of M-calpain.Schneider M, Ackermann K, Stuart M, Wex C, Protzer U, Schätzl HM, Gilch S.J Virol. 2012 Sep;86(18):10112-22. doi: 10.1128/JVI.01001-12. Proteasomal dysfunction and endoplasmic reticulum stress enhance trafficking of prion protein aggregates through the secretory pathway and increase accumulation of pathologic prion protein.Nunziante M, Ackermann K, Dietrich K, Wolf H, Gädtke L, Gilch S, Vorberg I, Groschup M, Schätzl HM.J Biol Chem. 2011 Sep 30;286(39):33942-53. doi: 10.1074/jbc.M111.272617. Chronic wasting disease.Gilch S, Chitoor N, Taguchi Y, Stuart M, Jewell JE, Schätzl HM.Top Curr Chem. 2011;305:51-77. doi: 10.1007/128_2011_159. Review. Prion protein interaction with stress-inducible protein 1 enhances neuronal protein synthesis via mTOR.Roffé M, Beraldo FH, Bester R, Nunziante M, Bach C, Mancini G, Gilch S, Vorberg I, Castilho BA, Martins VR, Hajj GN.Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13147-52. doi: 10.1073/pnas.1000784107. Electron paramagnetic studies of the copper and iron containing soluble ammonia monooxygenase from Nitrosomonas europaea.Gilch S, Meyer O, Schmidt I.Biometals. 2010 Aug;23(4):613-22. doi: 10.1007/s10534-010-9308-2. Inhibition of cholesterol recycling impairs cellular PrP(Sc) propagation.Gilch S, Bach C, Lutzny G, Vorberg I, Schätzl HM.Cell Mol Life Sci. 2009 Dec;66(24):3979-91. doi: 10.1007/s00018-009-0158-4. Prion-induced activation of cholesterogenic gene expression by Srebp2 in neuronal cells.Bach C, Gilch S, Rost R, Greenwood AD, Horsch M, Hajj GN, Brodesser S, Facius A, Schädler S, Sandhoff K, Beckers J, Leib-Mösch C, Schätzl HM, Vorberg I.J Biol Chem. 2009 Nov 6;284(45):31260-9. doi: 10.1074/jbc.M109.004382. A soluble form of ammonia monooxygenase in Nitrosomonas europaea.Gilch S, Meyer O, Schmidt I.Biol Chem. 2009 Sep;390(9):863-73. doi: 10.1515/BC.2009.085. Aptamers against prion proteins and prions.Gilch S, Schätzl HM.Cell Mol Life Sci. 2009 Aug;66(15):2445-55. doi: 10.1007/s00018-009-0031-5. Review. Therapy in prion diseases: from molecular and cellular biology to therapeutic targets.Krammer C, Vorberg I, Schätzl HM, Gilch S.Infect Disord Drug Targets. 2009 Feb;9(1):3-14. Autophagy induction by trehalose counteracts cellular prion infection.Aguib Y, Heiseke A, Gilch S, Riemer C, Baier M, Schätzl HM, Ertmer A.Autophagy. 2009 Apr;5(3):361-9. Interaction of the mechanism-based inactivator acetylene with ammonia monooxygenase of Nitrosomonas europaea.Gilch S, Vogel M, Lorenz MW, Meyer O, Schmidt I.Microbiology. 2009 Jan;155(Pt 1):279-84. doi: 10.1099/mic.0.023721-0.