COORSSEN, JENS - University of Calgary - Department of Biochemistry & Molecular Biology

个人简介

B.Sc. (Bachelor of Science) Ph.D. (Doctor of Philosophy)

研究领域

Delineation of the molecular mechanisms essential to exocytosis and endocytosis is critical to understanding a variety of disease states and to developing rational intervention strategies. My current work targets the Ca2+-triggered fusion steps. These studies utilize stage-specific model systems, permitting an integrated experimental approach combining techniques from physiology, proteomics, biochemistry, biophysics, molecular biology, and physical chemistry, together with specific, sensitive functional assays. Through collaborative work with colleagues at the NIH, these studies have shown that while certain proteins promote efficient exocytosis, they are not essential to the triggered fusion steps. By identifying essential conserved components, I hope to advance our understanding of synaptic function (including plasticity) and the regulation of secretion in other cell types. To this end, my collaborators and I at the University of Calgary, have also undertaken a functional proteomic analysis of the molecular mechanisms underlying simple forms of learning and memory.

近期论文

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Abbineni, P., Wright, E., Rogasevskaia, T., Killingsworth, M., Malladi, C. and Coorssen, J. (2014), 'The sea urchin egg and cortical vesicles as model systems to dissect the fast, Ca2+-triggered steps of regulated exocytosis', Neuromethods, vol 83 , pp 221 - 241. Rituper, B., Chowdhury, H., Jorgacevski, J., Coorssen, J., Kreft, M. and Zorec, R. (2013), 'Cholesterol-mediated membrane surface area dynamics in neuroendocrine cells', Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, vol 1831, no 7 , pp 1228 - 1238. Gauci, V., Padula, M. and Coorssen, J. (2013), 'Coomassie blue staining for high sensitivity gel-based proteomics', Journal of Proteomics, vol 90 , pp 96 - 106. Abbineni, P., Hibbert, J. and Coorssen, J. (2013), 'Critical role of cortical vesicles in dissecting regulated exocytosis : overview of insights into fundamental molecular mechanisms', Biological Bulletin, vol 224, no 3 , pp 200 - 217. Butt, R. and Coorssen, J. (2013), 'Coomassie Blue as a near-infrared fluorescent stain : a systematic comparison with Sypro Ruby for in-gel protein detection', Molecular and Cellular Proteomics, vol 12, no 12 , pp 3834 - 3850. Rogasevskaia, T., Churchward, M. and Coorssen, J. (2012), 'Anionic lipids in Ca?+ -triggered fusion', Cell Calcium, vol 52, no 3-4 , pp 259 - 269. Ormerod, K., Rogasevskaia, T., Coorssen, J. and Mercier, J. (2012), 'Cholesterol-independent effects of methyl-?-cyclodextrin on chemical synapses', PLoS ONE, vol 7, no 5 . Raveh, A., Valitsky, M., Shani, L., Coorssen, J., Blank, P., Zimmerberg, J. and Rahamimoff, R. (2012), 'Observations of calcium dynamics in cortical secretory vesicles', Cell Calcium, vol 52, no 3-4 , pp 217 - 225.