个人简介

Ph.D. in Chemistry, University of Padova, 1996 “Laurea” degree (B. Sc.) in Chemistry, University of Padova, 1992

研究领域

biophysical chemistry /structural biology /nmr spectroscopy /protein-protein interaction

Many processes in a living cell are regulated through the specific interaction between proteins. Targeting the disruption of protein-protein complexes for therapeutic purposes is considered a very attractive, albeit difficult, approach. In our research, we take advantage of information on the protein's overall fold, primary and secondary structure at the interface and flexibility to identify putative binding sites and design and test molecules that interfere with or block protein-protein interaction. We focus on peptides, peptidomimetics, and small molecular weight compounds.

近期论文

查看导师最新文章 （温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Song GJ, Leslie KL, Barrick S, Mamonova T, Fitzpatrick JM, Drombosky KW, Peyser N, Wang B, Pellegrini M, Bauer PM, Friedman PA, Mierke DF, Bisello A. "Phosphorylation of Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) by Akt Promotes Stability and Mitogenic Function of S-phase Kinase-associated Protein-2 (Skp2)." J Biol Chem. 2015 290(5):2879-87. doi: 10.1074/jbc.M114.609768. PMID: 25492869. http://www.ncbi.nlm.nih.gov/pubmed/25492869 Yatawara A, Gaidos G, Rupasinghe CN, O'Hara BA, Pellegrini M, Atwood WJ, Mierke DF. "Small-molecule inhibitors of JC polyomavirus infection." J Pept Sci. 2014 doi: 10.1002/psc.2731. PMID: 25522925. http://www.ncbi.nlm.nih.gov/pubmed/25522925 Guo B, Audu CO, Cochran JC, Mierke DF, Pellegrini M. Protein Engineering of the N-terminus of NEMO: structure stabilization and rescue of IKKβ binding. Biochemistry. 2014 53(43):6776-85. PMID: 25286246 DOI:10.1021/bi500861x. http://www.ncbi.nlm.nih.gov/pubmed/25286246 Guo B, Gurel PS, Shu R, Higgs HN, Pellegrini M, Mierke DF.Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR. Chem Commun (Camb). 2014 Sep 6;50(81):12037-9. doi: 10.1039/c4cc04399e. PMID: 25170530. http://www.ncbi.nlm.nih.gov/pubmed/25170530 Fitzpatrick JM, Pellegrini M, Cushing PR, Mierke DF. Small Molecule Inhibition of the Na+/H+ Exchange Regulatory Factor 1 and Parathyroid Hormone 1 Receptor Interaction. Biochemistry. 2014 ;53(37):5916-22. PMID: 25171053. http://www.ncbi.nlm.nih.gov/pubmed/25171053 Audu CO, Plati JJ, Pellegrini M, Mierke DF. Engineering a soluble parathyroid hormone GPCR mimetic. Proteins. 2014 Jul;82(7):1370-5. doi: 10.1002/prot.24503. Epub 2014 Jan 15. http://www.ncbi.nlm.nih.gov/pubmed/24375686 Audu, C. O.; Cochran, J. C.; Pellegrini, M.; Mierke, D. F. Recombinant Production of TEV Cleaved Human Parathyroid Hormone. (2013) Journal of Peptide Science, 19, 504–510. PMCID:PMC3755510. http://www.ncbi.nlm.nih.gov/pubmed/23794508 Pellegrini M, Willen L, Perroud M, Krushinskie D, Strauch K, Cuervo H, Day ES, Schneider P, Zheng TS.: Structure of the extracellular domains of human and xenopus Fn14: Implications in the evolution of TWEAK and Fn14 interactions. FEBS J. 2013 Apr;280(8):1818-29. http://www.ncbi.nlm.nih.gov/pubmed/23438059