个人简介

BS, University of South Carolina, 1999 PhD, University of Rochester, 2006 HHMI Postdoctoral Fellow, U of MD Baltimore County, 2006-2009 Assistant Professor of Chemistry, Miami University of OH, 2009-2012

研究领域

Biochemistry/Biophysical Chemistry/Structural Biology

Research in the Tolbert group endeavors to understand the molecular mechanisms RNA viruses use to express their genomes. Nuclear magnetic resonance (NMR) spectroscopy and other solution biophysical methods are used to determine 3D structures and physiochemical properties of viral RNA regulatory elements both free and bound to their cognate host proteins. The research in the group is highly interdisciplinary where trainees have the opportunity to gain experience in biophysical chemistry, molecular biology, computational biology, and virology. Our primary focus is to better understand how the human immunodeficiency virus (HIV), the etiological agent of AIDS, regulates RNA processing events. Towards that end, we determined the first high-resolution structure of a key HIV regulatory RNA, the Exon Splicing Silencer 3 (ESS3), and elucidated its thermodynamic binding profile to the human protein hnRNP A1. Other projects include determining the molecular mechanisms of transcriptional control of HIV latency and translational control of Enterovirus 71 (EV71), the etiological agent of Hand, Foot, and Mouth Disease.

近期论文

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Rollins C, Levengood JD, Rife BD, Salemi M, and Tolbert BS (2014). “Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element”. Biochemistry 53(13), 2172-2184. Levengood JD, Tolbert M, Li ML, and Tolbert BS (2013). “High-Affinity interactions of hnRNP A1 with conserved RNA structural elements is required for translation and replication of Enterovirus 71″. RNA Biology 10(6), 1-10 Blakeley BD, DePorter SM, Mohan U, Burai R, Tolbert BS, and McNaughton BR (2012). “Methods for identifying and characterizing interactions involving RNA”. Tetrahedron 68, 8837-8855. Feldman EA, Ni S, Sahu ID, Mishler CH, Levengood JD, Kushnir Y, McCarrick RM, Lorigan GA, Tolbert BS, Callahan SM, and Kennedy MA (2012). “Differential binding between PatS C-terminal peptide fragments and HetR from Anabaena sp. PCC 7120″. Biochemistry 51, 2436-42. Levengood JD, Rollins C, Mishler CH, Johnson CA, Miner G, Rajan P, Znosko BM, and Tolbert BS (2012). “Solution structure of the HIV-1 exon splicing silencer 3″. J Mol Biol. 414, 680-98. Feldmann EA, Ni S, Sahu ID, Mishler CH, Risser DD, Murakami JL, Tom SK, McCarrick RM, Lorigan GA, Tolbert BS, Callahan SM, and Kennedy MA (2011). “Evidence for direct binding between HetR from Anabaena sp. PCC 7129 and PatS-5″. Biochemistry 50, 9212-24. Miyazaki Y, Irobalieva RN, Tolbert BS, Smalls-Mantey A, Iyalla K, Loeliger K, D’Souza V, Khant H, Schmid MF, Garcia EL, Telesnitsky A, Chiu W, and Summers MF (2010). “Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography”. J Mol Biol. 404, 751-772 Tolbert BS, Miyazaki Y, Barton S, Kinde B, Stark P, Singh R, Bax A, Case D, Summers MF (2010). “Major groove width variations in RNA structures determined by NMR and impact of 13C residual chemical shift anisotropy and 1H-13C residual dipolar coupling on refinement”. J Biomol NMR, 47, 205-219.