个人简介

教育背景 1999 年 化学学士学位，复旦大学 2002 年 物理化学硕士学位，复旦大学化学系 导师：陆靖教授 论文题目：时间分辨荧光和拉曼光谱的理论研究 2002 - 2007 生物无机博士学位，加州理工学院化学系，Pasadena, CA, USA 导师：Professor Jacqueline K. Barton （美国科学院院士, 加州理工学院化学与化工学院院长） 论文题目：核酸中的电荷与电子传导 工作经历 2007-2010 博士后，哈佛大学医学院/麻省总医院，Cambridge, MA, USA 导师：Ralph Weissleder 教授 2010 至今 南洋助理教授，南洋理工大学数理学院化学与生物化学系，新加坡

研究领域

Biological Chemistry & Chemical Biology

The research interests of Dr. Shao’s group lie in the nucleic acids chemistry and the applications in the field of biochemistry, biology, medicine and nanotechnology. Current projects going on at our lab include: 1. Charge transport in nucleic acids. Aromatic π-stacking of nucleobases in various secondary structures of nucleic acids provide efficient pathway for electron conductivity. The process, called DNA-mediated charge transport, has promising ramification in biology and DNA nanotechnology. We are current investigating the characteristic features and the biological and technological applications of both oxidative and reductive DNA-mediated charge transport in B-form helical DNA and variety of non-canonical DNA structures, such as A-form duplex and four-stranded guanine quadruplexes. 2. Chemistry, Biology and Medicinal Applications of Guanine Quadruplexes. Guanine quadruplexes (GQ) are a family of four-stranded DNA structures, which attracts high interests in the field of anticancer therapies, due to their regulatory effects on the human telomerase and the transcription of many oncogenes. Guanine quadruplexes become promising targets for developing molecular anticancer drugs. At our lab, various types of organic compounds and inorganic complexes are developed to achieve not only the selectivity of GQ over duplex DNA but also specificity among the topological GQ structures. The outcome of the projects will provide promising anticancer therapeutic compounds and also useful tools for investigating GQ involved biological processes. 3. Epigenetic Effects on the Redox Chemistry of Nucleic Acids Epigenetic nucleobases have extra functional groups without disturbing the Watson-crick H-bondings and hence do not alter the primary structure of downstream proteins. The most common epigenetic base in human genome is 5-methyl-cytosine (mC). In the past two years, more derivatives of mC have been discovered in mammalian cells. These epigenetic cytosines provide an extra layer on genomic code, epigenetic code, which can modulate the chromatin conformation and downstream protein expression via active cytosine (de)methylation processes. At our lab, the chemical and biological properties of these epigenetic cytosines are exploring by various chemical, biochemical, and biological methods, in order to reveal their biological functions and explore the potential applications in the epigenetic therapy.

近期论文

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Zhang H., Ba S., Mahajan D., Lee J. Y., Ye R., Shao F., Lu L., Li T., "Versatile Types of DNA-Based Nanobiosensors for Specific Detection of Cancer Biomarker FEN1 in Living Cells and Cell-Free Systems" Nano Lett. 2018, Accepted. Zhu X., Wu J., Shao F.,* Hu X.,* “A Reversible Thermal Cycling of DNA Material for Efficient Cellulose Hydrolysis" ACS Appl. Bio Mater. 2018, 1(4) 1118-1123. He L. Sun X., Zhang H., Shao F* "G‐quadruplex Nanowires Direct the Efficiency and Selectivity of Electrocatalytic CO₂ Reduction" Angew. Chem. Int. Ed. 2018, doi: 10.1002/anie.201806652. Meng Z, Kubar T, Mu Y, Shao F* "A MD-QM Theoretical Study of DNA-Mediated Charge Transport in Hydrated Ionic Liquids" J Chem. Theory. Comput. 2018, 14 (5), pp 2733–2742. Wu Q, Amrutkar S, Shao F* "Sulfinate based selective labelling of 5-hydroxy methylcytosine: Application to biotin pull down assay" Bioconj. Chem. 2018 29(2)， 245-249. He L, Meng ZY, Xu D, Shao F* "Dual Functional Dinuclear Platinum Complex with Selective Reactivity towards c-myc G-quadruplex" Sci Rep 2018 767, doi:10.1038/s41598-017-19095-y Wang LY, Meng ZY, Martina F, Shao H, Shao F* "Fabrication of Circular Assemblies with DNA Tetrahedrons: From Static Structures to a Dynamic Rotary Motor" Nucleic Acid Res. 2017, 45(21), 12090-12099. Wu JY, Meng, ZY, Lu YP, Shao F* “Efficient Long-Range Charge Transport through G-quadruplex” Chem Eur J. 2017 23(56) 13980-13985. Peng Y, Huang Y, Zhu Y, Chen B, Wang L, Lai Z, Zhang Z, Zhao M, Tan C, Yang N, Shao F, Han Y, Zhang H, “Ultrathin Two-Dimensional Covalent Organic Framework Nanosheets: Preparation and Application in Highly Sensitive and Selective DNA Detection” J. Am. Chem. Soc. 2017, 139 (25), 8698–8704. Wong JR, Shao F* "Hole Transport in A-form DNA/RNA Hybrid Duplexes" Scientific Reports, 2017, 7, 40293. 37. He L, Meng Z, Chen X, Li T, Shao F* "Aza-bridged bisphenanthrolinyl Pt(II) complexes: efficient stabilization and topological selectivity on telomeric G-quadruplexes" J. Inorg. Biochem 2017, 166, 135-140. Xuan S, Meng Z, Wu X, Wong J, Devi G, Yeow E, Shao F*, "Efficient DNA-Mediated Electron Transport in Ionic Liquids" ACS Sus Chem & Eng 2016 4 6703-6711. He L, Chen X, Meng Z, Wang J, Tian K, Li T, Shao F*, "Octahedral ruthenium complexes selectively stabilize G-quadruplexes" Chem. Comm. 2016, 52, 8095-8098. Devi G, He L, Xu B, Li T, Shao F*, "In-stem thiazole orange reveals the same triplex intermediate for pH and thermal unfolding of i-motifs" Chem. Comm. 2016, 52, 7261-7264. Wu Q, Wong JR, Yeo LQ P, Zhang D, Shao F*, "Methylation on CpG repeats modulates hydroxymethylcytosine induced duplex destabilization" RSC Adv. 2016, 6, 48858-48862. Li T, Zhang H, Hu L, Shao F*, "Topoisomerase-Based Preparation and AFM Imaging of Multi-Interlocked Circular DNA" Bioconj. Chem, 2016, 27, 616-620. Wong JR, Lee K, Shu J, Shao F*, "Magnetic Fields Facilitate DNA-Mediated Charge Transport" Biochemistry, 2015 54(21), 3392-3399. Shu J, Wang Q, Yong K, Shao F, Lee K "Programmable DNA-Mediated Multitasking Processor" J Phys Chem 2015 119(17), 5639-5644. Xu B, Devi G, Shao F*, "Regulation of Telomeric i-motif Stability by 5-methylcytosine and 5-hydroxymethylcytosine Modification" Org Biomol Chem, 2015, 13, 5646 - 5651. You H, Wu J, Shao F, Yan, J "Stability and kinetics of c-MYC promoter G-quadruplexes studied by single-molecule manipulation" J. Am. Chem. Soc. 2015, 137 (7), 2424–2427. Xuan S, Wu Q, Cui L, Shao F*, "5-Hydroxymethylcytosine and 5-Formylcytosine Containing Deoxyoligonucleotides: Facile Syntheses and Melting Temperature Studies" Bioorg. Med. Chem. Lett. 2015, 25, 1186–1191.