Hislop, James - University of Aberdeen - School of Medicine, Medical Sciences and Nutrition

个人简介

I received my B.Sc in Pharmacology from the University of Bristol in 1997, before going on to undertake a Ph.D in the department of Medicine, also at the University of Bristol, under the supervision of Prof Craig McArdle, where I investigated the role of endocytosis in the function of the Gonadotropin Releasing Hormone Receptor. On completion of my PhD, I took a postdoctoral position at the University of California, San Francisco, where I continued my training in the laboratory of Prof Mark von Zastrow, a renowned leader in the field of endocytic trafficking in the function of G-protein-coupled receptors, where I investigated the role of Ubiquitin in endosomal trafficking. In 2011, I returned to the UK to work in the Gene Therapy Group at Imperial College London, in the laboratory of Prof Nicholas Mazarakis, where I studied how lentiviral vectors undergo trafficking within motor neurones. I was appointed Lecturer in Pharmacology at the University of Aberdeen in January 2013

研究领域

My overall research involves investigating the mechanisms by which G-protein-coupled Receptors undergo endocytic trafficking and how this process influences receptor function and cellular signalling. G-protein coupled receptors (GPCRs), represent the largest family of cell surface receptorsand are the prime regulators of many physiological systems. Their undoubted importance is demonstrated by the fact that as many as 50% of marketed drugs target GPCRs. The signalling fidelity of GPCRs is maintained in part by a regulated trafficking process involving the removal of desensitised receptors by internalisation followed by either recycling to the plasma membrane (resensitisation) or targeting to the lysosome for downregulation. Although a great deal of progress has been made in understanding the processes underlying receptor endocytosis, including the critical role of receptor kinases and arrestin, comparatively little is known about the processes determining the postendocytic fate of GPCRs. My specific interests lie in how the levels of receptor are maintained or downregulated in the extended presence of agonist, and what mechanisms determine how receptors are sorted to the lysosome.

近期论文

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Thompson, D., McArthur, S., Hislop, JN., Flower, RJ. & Perretti, M. (2014). 'Identification of a novel recycling sequence in the C-tail of FPR2/ALX: association with cell protection from apoptosis'. The Journal of Biological Chemistry, vol 289, pp. 36166-36178. DOI: [ONLINE] DOI: 10.1074/JBC.M114.612630 [ONLINE] AURA: 36166.FULL.PDF Hislop, JN., Islam, TA., Eleftheriadou, I., Carpentier, DCJ., Trabalza, A., Parkinson, M., Schiavo, G. & Mazarakis, ND. (2014). 'Rabies virus envelope glycoprotein targets lentiviral vectors to the axonal retrograde pathway in motor neurons'. The Journal of Biological Chemistry, vol 289, no. 23, pp. 16148-16163. DOI: [ONLINE] DOI: 10.1074/JBC.M114.549980 Trabalza, A., Georgiadis, C., Eleftheriadou, I., Hislop, JN., Ellison, SM., Karavassilis, ME. & Mazarakis, ND. (2013). 'Venezuelan equine encephalitis virus glycoprotein pseudotyping confers neurotropism to lentiviral vectors'. Gene Therapy, vol 20, no. 7, pp. 723-732. DOI: [ONLINE] DOI: 10.1038/GT.2012.85 Henry, AG., Hislop, JN., Grove, J., Thorn, K., Marsh, M. & von Zastrow, M. (2012). 'Regulation of endocytic clathrin dynamics by cargo ubiquitination'. Developmental Cell, vol 23, no. 3, pp. 519-532. DOI: [ONLINE] DOI: 10.1016/J.DEVCEL.2012.08.003 [ONLINE] AURA: 1_S2.0_S153458071200367X_MAIN.PDF Hislop, JN., Henry, AG. & von Zastrow, M. (2011). 'Ubiquitination in the first cytoplasmic loop of μ-opioid receptors reveals a hierarchical mechanism of lysosomal down-regulation'. The Journal of Biological Chemistry, vol 286, no. 46, pp. 40193-40204. DOI: [ONLINE] DOI: 10.1074/JBC.M111.288555 Henry, AG., White, IJ., Marsh, M., von Zastrow, M. & Hislop, JN. (2011). 'The role of ubiquitination in lysosomal trafficking of δ-opioid receptors'. Traffic, vol 12, no. 2, pp. 170-184. DOI: [ONLINE] DOI: 10.1111/J.1600-0854.2010.01145.X Hislop, JN. & von Zastrow, M. (2011). 'Role of ubiquitination in endocytic trafficking of G-protein-coupled receptors'. Traffic, vol 12, no. 2, pp. 137-148. DOI: [ONLINE] DOI: 10.1111/J.1600-0854.2010.01121.X Hislop, JN., Henry, AG., Marchese, A. & von Zastrow, M. (2009). 'Ubiquitination regulates proteolytic processing of G protein-coupled receptors after their sorting to lysosomes'. The Journal of Biological Chemistry, vol 284, no. 29, pp. 19361-19370. DOI: [ONLINE] DOI: 10.1074/JBC.M109.001644 Tanowitz, M., Hislop, JN. & von Zastrow, M. (2008). 'Alternative splicing determines the post-endocytic sorting fate of G-protein-coupled receptors'. The Journal of Biological Chemistry, vol 283, no. 51, pp. 35614-35621. DOI: [ONLINE] DOI: 10.1074/JBC.M806588200 Hislop, JN., Caunt, CJ., Sedgley, KR., Kelly, E., Mundell, S., Green, LD. & McArdle, CA. (2005). 'Internalization of gonadotropin-releasing hormone receptors (GnRHRs): does arrestin binding to the C-terminal tail target GnRHRs for dynamin-dependent internalization?'. Journal of Molecular Endocrinology, vol 35, no. 1, pp. 177-89. DOI: [ONLINE] DOI: 10.1677/JME.1.01809