Frigerio, Lorenzo - University of Warwick - Department of Biotechnology

个人简介

PhD Plant Genetics. Universita Cattolica del Sacro Cuore 1995

研究领域

Most plant proteins of interest for human and animal nutrition, or of biotechnological value are located in, or travel through, the secretory pathway. After synthesis, secretory proteins are translocated into the endoplasmic reticulum (ER), where they acquire their mature tertiary and quaternary structure. They are then transported along the secretory system to their final destinations: the ER itself, the Golgi complex, the 'endosomes', the vacuoles, the plasma membrane or the extracellular space. Our research group is interested in three major processes that regulate the plant secretory system: Sorting of proteins to plant storage vacuoles: We have isolated two novel vacuolar sorting signals (from ricin and phaseolin) and are now analysing their ability to interact with vacuolar sorting receptors. We use a combination of biochemistry, genetics and live confocal microscopy. We have recently generated a panel of fluorescent reporter proteins targeted to the vacuolar lumen or the vacuolar membrane. These are being used to study vacuolar biogenesis and targeting in developing Arabidopsis seeds. As part of the EU 'Pharma-Planta' consortium, we are also studying the intracellular targeting and fate of recombinant protein of medical importance, in particular monoclonal antibodies and HIV antigens. ER quality control and protein degradation: how does the endoplasmic reticulum dispose of proteins that fail to fold or assemble correctly? Where does degradation occur? Which molecular chaperones are involved in the degradative process? How does the plant ER handle the synthesis of heterologous proteins with complex folding/assembly patterns, such as immunoglobulins? In collaboration with the Toxin Group, these questions are addressed in vivo by studying the intracellular fate of model secretory proteins, such as the plant toxin ricin, a secretory immunoglobulin A and the storage protein phaseolin. These studies employ biochemistry, cell biology and proteomic techniques. ER shape and function: we have recently initiated the study of the plant reticulon family. Reticulons have been described as major regulators of ER shape in mammalian and yeast cells. The Arabidopsis genome contains 21 members of the reticulon family. We analysed the smallest isoform, RTNLB13, and found that its overexpression in plant cells constricts the ER tubules, thus limiting diffusion of soluble proteins in the ER lumen. In collaboration with Prof Chris Hawes (Oxford Brookes), we are now assessing the effect of reticulon overexpression on the overall function of the anterograde secretory pathway

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Lopez, David, Amira, Maroua Ben, Brown, Daniel, Muries, Beatriz, Brunel-Michac, Nicole, Bourgerie, Sylvain, Porcheron, Benoit, Lemoine, Remi, Chrestin, Hervé, Mollison, Ewan, Di Cola, Alessandra, Frigerio, Lorenzo, Julien, Jean-Louis, Gousset-Dupont, Aurélie, Fumanal, Boris, Label, Philippe, Pujade-Renaud, Valérie, Auguin, Daniel, Venisse, Jean-Stéphane. 2016. The Hevea brasiliensis XIP aquaporin subfamily : genomic, structural and functional characterizations with relevance to intensive latex harvesting. Plant Molecular Biology, 91 (4), pp. 375-396, View Reinhardt , Hagen, Hachez , Charles, Bienert, Manuela Désirée, Beebo , Azeez, Swarup , Kamal, Voss , Ute, Bouhidel , Karim, Frigerio, Lorenzo, Schjoerring, Jan K., Bennett , Malcolm J., Chaumont , Francois. 2016. Tonoplast aquaporins facilitate lateral root emergence. Plant Physiology, 170 (3), pp. 1640-1654, View Breeze, Emily, Dzimitrowicz, Natasha, Kriechbaumer, Verena, Brooks, Rhiannon, Botchway, Stanley W., Brady, Jacob P., Hawes, Chris, Dixon, Ann, Schnell, Jason R., Fricker, Mark D., Frigerio, Lorenzo. 2016. A C-terminal amphipathic helix is necessary for the in-vivo tubule-shaping function of plant reticulon. Proceedings of the National Academy of Sciences of the United States of America, 113 (39), pp. 10902-10907, View Knox, Kirsten, Wang, Pengwei, Kriechbaumer, Verena, Tilsner, Jens, Frigerio, Lorenzo, Sparkes, Imogen, Hawes, C. R., Oparka, Karl J.. 2015. Putting the squeeze on PDs : a role for RETICULONS in primary plasmodesmata formation. Plant Physiology, 168 (4), pp. 1563-1572, View Kriechbaumer, Verena, Botchway, Stanley W., Slade, Susan E., Knox, Kirsten, Frigerio, Lorenzo, Oparka, Karl J., Hawes, Chris. 2015. Reticulomics : protein-protein interaction studies with two plasmodesmata-localised reticulon family proteins identify binding partners enriched at plasmodesmata, ER and the plasma membrane. Plant Physiology, 169 (3), pp. 1933-1945, View Battelli, Riccardo, Lombardi, Lara, Picciarelli, Piero, Lorenzi, Roberto, Frigerio, Lorenzo, Rogers, Hilary J.. 2014. Expression and localisation of a senescence-associated KDEL-cysteine protease from Lilium longiflorum tepals. Plant Science, Volume 214, pp. 38-46, View Brandizzi, Federica, Frigerio, Lorenzo, Howell, Stephen H., Schäfer, Patrick. 2014. Endoplasmic reticulum : shape and function in stress translation. Frontiers in Plant Science, Volume 5, pp. 1-2, View Feeney, Mistianne, Frigerio, Lorenzo, Cui, Y., Menassa, Rima. 2013. Following vegetative to embryonic cellular changes in leaves of arabidopsis thaliana over-expressing LEAFY COTYLEDON2. Plant Physiology, Volume 162 (Number 4), pp. 1881-1896, View