Smith, Corinne - University of Warwick - Department of Biomedical Science

个人简介

PhD University of Bristol 1992 1992 - 1995 Post-doctoral Research Associate, University of Bristol 1995 - 1999 Research Associate, MRC Laboratory of Molecular Biology, Cambridge 1999 - 2002 Research associate and MRC Career Development Fellow, Birkbeck College, London 2002 - 2004 MRC Career Development Fellow, University of Warwick 2004 - 2007 Assistant Professor, University of Warwick 2007 - now Associate Professor, University of Warwick

研究领域

The research in my laboratory involves investigating the structure and mechanism of clathrin coated vesicle formation during clathrin-mediated endocytosis using a range of structural and biophysical techniques. Receptor-mediated endocytosis allows a cell to choose and absorb the substances it requires. During endocytosis a clathrin-coated vesicle is formed which contains only specifically selected cargo. The clathrin coat is central to the ability of the vesicle to choose its contents and we aim, through a combination of structural and biophysical analysis to determine the factors that control its formation and disassembly We are currently investigating how the molecular chaperone, Hsc70, and its cofactor auxilin, cause clathrin cage disassembly in vitro. We are using two approaches to investigate this. In the first we use 3D cryo-electron microscopy techniques to analyse the structure of purified clathrin cages bound to a range of proteins including Hsc70 and auxilin. In the second approach we monitor the kinetics of the process of clathrin cage disassembly using a range of biophysical techniques such as fluorescence anisotropy, stopped flow kinetics, isothermal titrating calorimetry and light scattering. We are now broadening our studies to investigate the part played by adaptor proteins and nucleotide exchange factors in the formation and disassembly of clathrin cage complexes.

近期论文

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Millard, Christopher J., Varma, Niranjan, Saleh, Almutasem, Morris, Kyle L., Watson, Peter J., Bottrill, Andrew R., Fairall, Louise, Smith, Corinne J., Schwabe, John W. R.. 2016. The structure of the core NuRD repression complex provides insights into its interaction with chromatin. eLife, 5, pp. 1-21, View Barry, Nicolas P. E., Pitto-Barry, Anaà¯s, Sanchez, Ana M., Dove, Andrew P., Procter, Richard J., Soldevila-Barreda, Joan J., Kirby, Nigel, Hands-Portman, Ian, Smith, Corinne J., O'Reilly, Rachel K., Beanland, R., Sadler, P. J.. 2014. Fabrication of crystals from single metal atoms. Nature Communications, Volume 5, View Patel, Roshani, Vasilev, Cvetelin, Beck, Daniel, Monteferrante, Carmine G., Dijl, Jan Maarten van, Hunter, C. Neil, Smith, Corinne J., Robinson, Colin. 2014. A mutation leading to super-assembly of twin-arginine translocase (Tat) protein complexes. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1843 (Number 9), pp. 1978-1986, View Barry, Nicolas P. E., Pitto-Barry, Anaà¯s, Romero-Canelà³n, Isolda, Tran, Johanna, Soldevila-Barreda, Joan J., Hands-Portman, Ian, Smith, Corinne J., Kirby, Nigel, Dove, Andrew P., O'Reilly, Rachel K., Sadler, P.J. 2014. Precious metal carborane polymer nanoparticles : characterisation of micellar formulations and anticancer activity. Faraday Discussions, 175, pp. 229-240, View Beck, Daniel, Vasisht, Nishi, Baglieri, J., Monteferrante, Carmine G., Dijl, Jan Maarten van, Robinson, Colin, Smith, Corinne J.. 2013. Ultrastructural characterisation of Bacillus subtilis TatA complexes suggests they are too small to form homooligomeric translocation pores. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1833 (Number 8), pp. 1811-1819, View Pacheco-Gà³mez, Raàºl, Cheng, Xi, Hicks, Matthew R., Smith, Corinne J., Roper, David I., Addinall, Stephen G., Rodger, Alison, Dafforn, Tim. 2013. Tetramerization of ZapA is required for FtsZ bundling. Biochemical Journal, Volume 449 (Number 3), pp. 795-802, View Young, Anna, Stoilova-McPhie, Svetla, Rothnie, Alice, Vallis, Yvonne, Harvey-Smith, Phillip, Ranson, Neil, Kent, Helen, Brodsky, Frances M., Pearse, Barbara M. F., Roseman, Alan, Smith, Corinne J.. 2013. Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. Traffic, Volume 14 (Number 9), pp. 987-996, View Baglieri, J., Beck, D., Vasisht, Nishi, Smith, Corinne J., Robinson, Colin. 2012. Structure of TatA paralog, TatE, suggests a tructurally homogeneous form of Tat protein translocase that transports folded proteins of differing diameter. Journal of Biological Chemistry, Vol.287 (No.10), pp. 7335-7344, View Turner, Daniel J., Portman, Ian, Dafforn, Tim, Rodger, Alison, Roper, David I., Smith, Corinne J., Turner, Matthew S.. 2012. The Mechanics of FtsZ fibers. Biophysical Journal, Vol.102 (No.4), pp. 731-738, View