Coldwell, Mark J - University of Southampton - Discipline of Biological Sciences

个人简介

Career History 2008-Present: Lecturer in Biochemistry. University of Southampton, UK. 2008-Present: Biochemical Society Local Ambassador for University of Southampton, UK. 2001-2008: Postdoctoral Research Fellow at the University of Sussex, UK. Academic Qualifications 1997-2001: PhD in Biochemistry. University of Leicester, UK. 1994-1997: BSc (Hons) in Biological Sciences (Molecular Biology). University of Leicester, UK.

研究领域

Throughout my research career, I have been investigating eukaryotic post-transcriptional control, in particular the initiation phase of translation. This key point in gene expression can rapidly alter the temporal and spatial expression of either the whole transcriptome or specific mRNAs, without requiring new transcription. Central to translation initiation is the selection of the initiation codon, which is usually an AUG. However, other codons may be used for translation giving rise to N-terminally extended or truncated polypeptides. We are investigating the usage and regulation of these alternative initiation codons in order to examine the importance of alternative initiation codon selection in the generation of protein isoform diversity, a previously neglected aspect of gene expression. This work will modify and extend our understanding of the protein-coding potential of all eukaryotic genomes, as the underlying mechanisms of translational control are conserved across species. Selection of the correct initiation codon is mediated by several initiation factors, and part of this project explores the regulation of AUG usage versus non-AUG initiation codons. It is clear that the selection of certain initiation codons may have beneficial or detrimental effects on the cell and it is important to establish in which stages of cell growth and/or disease progression that this form of translational control occurs. Deregulation of the appropriate selection of translation initiation codons may be important in the status of certain diseases, including cancer.

近期论文

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PEITC-mediated inhibition of mRNA translation is associated with both inhibition of mTORC1 and increased eIF2alpha phosphorylation in established cell lines and primary human leukemia cells - Yeomans, Alison, Lemm, Elizabeth, Wilmore, Sarah, Cavell, Breeze, Valle-Argos, Beatriz, Krysov, Sergey, Hidalgo, Marina, Leonard, Elodie, Willis, Anne E., Forconi, Francesco, Stevenson, Freda, Steele, Andrew, Coldwell, Mark and Packham, Graham Published:2016Publication:OncotargetPage Range:1-13doi:10.18632/oncotarget.11655PMID:27579538 Engagement of the B-cell receptor of chronic lymphocytic leukemia cells drives global and MYC-specific mRNA translation. - Yeomans, Alison, Thirdborough, Stephen M., Valle-Argos, Beatriz, Linley, Adam, Krysov, Sergey, Hidalgo, Marina Sanchez, Leonard, Elodie, Ishfaq, Muhammad, Wagner, Simon D., Willis, Anne E., Steele, Andrew, Stevenson, Freda, Forconi, Francesco, Coldwell, Mark J. and Packham, Graham Published:2015Publication:BloodVolume:127, (4)Page Range:449-57doi:10.1182/blood-2015-07-660969PMID:26491071 ABC50 mutants modify translation start codon selection - Stewart, Joanna D, Cowan, Joanne L., Perry, Lisa S., Coldwell, Mark J. and Proud, Christopher G. Published:2015Publication:The Biochemical JournalPage Range:1-25doi:10.1042/BJ20141453PMID:25597744 mTORCing about myogenic differentiation - Iadevaia, Valentina, Cowan, Joanne L. and Coldwell, Mark J. Published:2014Publication:Cell CycleVolume:14, (1)Page Range:3-4doi:10.4161/15384101.2014.988107PMID:25551658 Phosphorylation of eIF4GII and 4E-BP1 in response to nocodazole treatment: a reappraisal of translation initiation during mitosis - Coldwell, Mark J., Cowan, Joanne L., Vlasak, Markete, Mead, Abbie, Willett, Mark, Perry, Lisa S. and Morley, Simon J. Published:2013Publication:Cell CycleVolume:12, (23)Page Range:1-14doi:10.4161/cc.26588PMID:24091728