Bunning, Rowena - Sheffield Hallam University - Department of Biosciences and Chemistry

个人简介

I was appointed as a Senior Lecturer in Cell Biology at Sheffield Hallam University in 1989 after having worked as a Postdoctoral Research Associate at the Department of Human Metabolism and Clinical Biochemistry, University of Sheffield for 6 years and prior to that for three years at the Strangeways Research Laboratory in Cambridge. I teach Cell Biology and Immunology and oversee the Biosciences and Chemistry MSc programme as Academic Delivery Manager. My research interests are focussed around mechanisms of extracellular matrix breakdown and how it can be prevented.

研究领域

The effects of cannabinoids on chondrocyte metabolism In addition to their well characterised psychotic effects, cannabinoids demonstrate analgesic, anti-inflammatory and immunosuppressive properties and have been observed to reduce joint damage in animal models of arthritis. We are interested in determining whether cannabinoids have any direct action on chondrocytes, which may be chondroprotective and account for some of their observed anti-arthritic effects. Our studies have shown inhibition of cytokine-stimulated nitric oxide production by cannabinoids R-(+)-win 55, 212-2 (win-55) and HU210 and inhibition of cytokine-stimulated cartilage proteoglycan and collagen breakdown in bovine cartilage and chondrocytes. More recently we have shown win-55 inhibits basal and IL-1β stimulated matrix metalloproteinase -3 and -13 production at the gene and protein level in human osteoarthritic chondrocytes. These results suggest the potential use of cannabinoids as anti-arthritic agents. The role of ADAMTS-1, -4, -5 and -9 in the pathogenesis of multiple sclerosis (MS) The ADAMTS (a disintegrin and metalloproteinase with thrombospondin type motif) group of enzymes are secreted enzymes. ADAMTS -1, -4, -5 and -9 are aggrecanases thought to be the main enzymes responsible for aggrecan breakdown in cartilage. The brain also has aggrecan-like proteoglycans in its extracellular matrix therefore these ADAMTSs may be involved in extracellular matrix breakdown in MS, allowing infiltration of damaging inflammatory cells. ADAMTS-1, -4 and -5 mRNA and protein have been demonstrated to be present in normal and MS white matter. We are now studying the expression of ADAMTS-9 in MS brain tissue and the role of ADAMTSs in breakdown of brain extracellular matrix, in particular the presence of versican and aggrecan neoepitopes produced by ADAMTS cleavage in normal and MS tissue as an indicator of in vivo ADAMTS activity.

近期论文

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Wang JR, Tian Y, Phillips KLE, Chiverton N, Haddock G, Bunning RAD, Cross AK, Shapiro IM, Le Maitre CL, Risbud MV (2013) Tumour necrosis factor and interleukin-1 dependent induction of CCL3 expression by nucleus pulposus cells promotes macrophage migration through CCR1. Arthritis and Rheumatism 65 (3) 832-842. Turner SL, Blair-Zajdel ME, Bunning RAD (2009) ADAMs and ADAMTSs in cancer. The British Journal of Biomedical Science 66, 117-127. Hurst LA, Bunning RAD, Couraud P-O, Romero IA, Weksler BB, Sharrack B, Woodroofe MN (2009) Expression of ADAM-17, TIMP3 and fractalkine in the human brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. Journal of Neuroimmunology 210, 108-112. Reid MJ, Cross AK, Haddock G, Allan SM, Stock CJ, Woodroofe NM, Buttle DJ, Bunning RAD (2009) ADAMTS-9 expression is up-regulated following transient middle cerebral artery occlusion (tMCAo) in the rat. Neuroscience Letters 452, 252-257. DUNN, Sara L., WILKINSON, Jeremy Mark, CRAWFORD, Aileen, BUNNING, Rowena A.D. and LE MAITRE, Christine L. (2016). Expression of cannabinoid receptors in human osteoarthritic cartilage: implications for future therapies. Cannabis and Cannabinoid Research, 1 (1), 3-15.