Wilderspin, Andy - University College London

个人简介

BSc Hons in Biochemistry, 2.1, University of Southampton, 1980 PhD in Biochemistry, National Institute for Medical Research, Mill Hill, London, 1984

研究领域

Cloning, mutagenesis, expression and purification of recombinant proteins for structural analysis and structure-based drug design, including STAT3, HIF-1, p300, HIVPR, PDE3 Structural analysis and DNA binding of the transcription factor STAT3 in both the phosphorylated and unphosphorylated state Design and use of fluorescence-based assays eg FRET, fluorescence polarisation and PEMSA to screen for inhibitors of protein-protein interactions Development of Nrf2 and Keap-1 protein-protein interaction assays in collaborative work with Dr Geoff Wells

近期论文

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Crystallization and preliminary X-ray investigation of recombinant simian immunodeficiency virus proteinase Journal article ; 1993 ; Journal of Molecular Biology links: IRIS ; UCL Discovery authors : Wilderspin AF,Sugrue RJ Development of a steady-state FRET-based assay to identify inhibitors of the Keap- 1-Nrf2 protein-protein interaction Journal article ; 2013 ; Protein Science links: IRIS ; UCL Discovery ; publisher authors [click to collapse] : Schaap Schapp M,Hancock R,Wilderspin A,Wells G Development of a steady-state FRET-based assay to identify inhibitors of the Keap1-Nrf2 protein-protein interaction Journal article ; 2013 ; Protein Science links: IRIS ; UCL Discovery ; doi ; publisher authors [click to collapse] : Schaap M,Hancock R,Wilderspin A,Wells G Investigation of the protein alkylation sites of the STAT3:STAT3 inhibitor Stattic by mass spectrometry Journal article ; 2013 ; Bioorganic and Medicinal Chemistry Letters links: IRIS ; UCL Discovery ; doi authors [click to collapse] : Heidelberger S,Zinzalla G,Antonow D,Essex S,Piku Basu B,Palmer J,Husby J,Jackson PJM,Rahman KM,Wilderspin AF,Zloh M,Thurston DE Investigation of the protein alkylation sites of the STAT3:STAT3 inhibitor Stattic by mass spectrometry Journal article ; 2013 ; Bioorganic and Medicinal Chemistry Letters links: IRIS ; UCL Discovery authors [click to collapse] : Heidelberger S,Zinzalla G,Antonow D,Essex S,Piku Basu B,Palmer J,Husby J,Jackson PJM,Rahman KM,Wilderspin AF,Zloh M,Thurston DE Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography Journal article ; 2013 ; FEBS Letters links: IRIS ; UCL Discovery ; doi authors [click to collapse] : Nkansah E,Shah R,Collie GW,Parkinson GN,Palmer J,Rahman KM,Bui TT,Drake AF,Husby J,Neidle S,Zinzalla G,Thurston DE,Wilderspin AF Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography. Journal article ; 2013 ; FEBS Lett ; England links: IRIS ; UCL Discovery ; doi ; author's authors [click to collapse] : Nkansah E,Shah R,Collie GW,Parkinson GN,Palmer J,Rahman KM,Bui TT,Drake AF,Husby J,Neidle S,Zinzalla G,Thurston DE,Wilderspin AF Protein transduction by lipidic peptide dendrimers Journal article ; 2006 ; Journal of Pharmaceutical Sciences links: IRIS ; UCL Discovery ; doi ; publisher authors [click to collapse] : Bayele HK,Ramaswamy C,Wilderspin AF,Srai KS,Toth I,Florence AT Purification and kinetic properties of cardiac calmodulin dependent cyclic nucleotide PDE (PDE-I) Journal article ; 1996 ; Biochemical Society Transactions links: IRIS ; UCL Discovery authors : Clapham JC,Wilderspin AF 97 Characterisation of drug resistant retroviral proteinase mutants Journal article ; 1997 ; Biochemical Society Transactions links: IRIS ; UCL Discovery authors [click to collapse] : Good VM,Anderson MM,Baker JJ,Moloudi A,James CH,Wilderspin AF Cloning of cyclic nucleotide phospodiesterase type I gene into the vector pPICαC for secreted expression in Pichia pastoris Journal article ; 1999 ; Journal of Pharmacy and Pharmacology links: IRIS ; UCL Discovery authors [click to collapse] : Sidhu R,Clapham JC,Wilderspin AF Investigation of transfection and cell viability using high concentrations of lipidic dendrimeric lysine Journal article ; 1999 ; Journal of Pharmacy and Pharmacology links: IRIS ; UCL Discovery authors [click to collapse] : Shah DS,Sakthivel T,Toth I,Florence AT,Wilderspin AF Cloning and expression of cyclic nucleotide phosphodiesterase type 1 gene in E. coli Journal article ; 2000 ; Journal of Pharmacy and Pharmacology links: IRIS ; UCL Discovery authors [click to collapse] : Sidhu R,Clapham JC,Wilderspin AF Characterisation of drug resistant retroviral proteinase mutants. Journal article ; 1997 ; Biochem Soc Trans ; England links: IRIS ; UCL Discovery ; author's authors [click to collapse] : Good VM,Anderson MM,Baker JJ,Moloudi A,James CH,Wilderspin AF Autocatalytic processing of mutant SIV-PR polyproteins. Journal article ; 1998 ; Biochem Soc Trans ; England links: IRIS ; UCL Discovery ; author's authors [click to collapse] : Moloudi A,Good VM,Anderson MM,Baker JJ,James CH,Wilderspin AF Alternative Native Flap Conformation Revealed by 2�3 � Resolution Structure of SIV Proteinase Journal article ; 1994 ; Journal of Molecular Biology links: IRIS ; UCL Discovery ; doi ; publisher authors : Wilderspin AF,Sugrue RJ Novel cationic lipidic peptide dendrimer vectors in vitro gene delivery Journal article ; 1999 ; STP Pharma Sciences links: IRIS ; UCL Discovery authors [click to collapse] : Toth I,Sakthivel T,Wilderspin AF,Bayele H,O'Donnell M,Perry K,Pasi KJ,Lee CA,Florence AT DNA transfection and transfected cell viability using amphipathic asymmetric dendrimers Journal article ; 2000 ; International Journal of Pharmaceutics links: IRIS ; UCL Discovery ; doi ; publisher authors [click to collapse] : Shah DS,Sakthivel T,Toth I,Florence AT,Wilderspin AF Dendriplexes and their characterisation Journal article ; 2003 ; International Journal of Pharmaceutics links: IRIS ; UCL Discovery ; doi ; publisher authors [click to collapse] : Ramaswamy C,Sakthivel T,Wilderspin AF,Florence AT A proteomic approach to the identification of cytochrome P450 isoforms in male and female rat liver by nanoscale liquid chromatography-electrospray ionization-tandem mass spectrometry Journal article ; 2004 ; Drug Metabolism and Disposition links: IRIS ; UCL Discovery authors [click to collapse] : Nisar S,Lane CS,Wilderspin AF,Welham KJ,Griffiths WJ,Patterson LH Solid-Phase Synthesis of Chlorofusin Analogues Journal article ; 2007 ; Journal of Organic Chemistry links: IRIS ; UCL Discovery ; doi ; publisher authors [click to collapse] : Woon ECY,Arcieri M,Wilderspin AF,Malkinson JP,Searcey M Identification of an inhibitory Zn2+ binding site on the human glycine receptor alpha1 subunit Journal article ; 1999 ; Journal of Physiology links: IRIS ; UCL Discovery ; doi ; publisher authors [click to collapse] : Harvey RJ,Thomas P,James CH,Wilderspin AF,Smart TG Investigation of the association and flexibility of cationic lipidic peptide dendrons by NMR spectroscopy. Journal article ; 2005 ; Magn Reson Chem ; England links: IRIS ; UCL Discovery ; doi ; author's authors [click to collapse] : Zloh M,Ramaswamy C,Sakthivel T,Wilderspin A,Florence AT A novel small-molecule inhibitor of IL-6 signalling. Journal article ; 2010 ; Bioorganic & Medicinal Chemistry Letters links: IRIS ; doi ; publisher authors [click to collapse] : Zinzalla G,Haque MR,Basu BP,Anderson J,Kaye SL,Haider SM,Hasan F,Antonow D,Essex S,Rahman KM,Palmer J,Morgenstern D,Wilderspin AF,Neidle S,Thurston DE Versatile peptide dendrimers for nucleic acid delivery. Journal article ; 2005 ; J Pharm Sci ; United States links: IRIS ; UCL Discovery ; doi ; author's authors [click to collapse] : Bayele HK,Sakthivel T,O'Donell M,Pasi KJ,Wilderspin AF,Lee CA,Toth I,Florence AT