个人简介

Research Fellow, Department of Bacteriology, University of Bristol, 1979-1980 Research Fellow, Department of Biochemistry and Applied Molecular Biology, UMIST, 1981-1983 Lecturer, Department of Biochemistry, UCL, 1983-1992 Senior Lecturer, Department of Biochemistry and Molecular Biology, UCL, 1992-2003 Reader, Department of Biochemistry and Molecular Biology, UCL, 2003-2006 Professor of Molecular Microbiology, Department of Biochemistry and Molecular Biology, UCL, 2006-2012 Professor of Synthetic Biology for Bioprocessing, Department of Biochemical Engineering, UCL, 2012-present. 2012-present, UK representative to COST 2010 IChemE Innovation and Excellence Award 2010 Rita & John Cornforth Award from the Royal Society of Chemistry 2010-present, pool member BBSRC Committee B 2007-present, member of the Society for Applied Microbiology 2005-present, member of Centre of Excellence in Biocatalysis, Biotransformation and Biomanufacturing (CoEBio3) 2004-2007 member of the Pro-Bio Faraday Partnership 1986-present, member of the Biochemical Society 1983-present, member of the American Society of Microbiology 1982-present, member of the Society for General Microbiology

研究领域

John Ward’s research interests are in the use of bacteria their enzymes and phages to develop new chemicals, pathways and active entities for applications in synthetic biology, biocatalysis and host cell engineering for bioprocessing.John has developed the molecular biology and biochemistry to access many enzymes for biocatalysis for the Bioconversion-Chemistry-Engineering (BiCE) programme, these enzymes include dioxygenases, transketolase, Baeyer-Villiger mono-oxygenases, cytochrome P450's, transaminases and synthetic plant enzymes such as norcoclaurine synthase. The aim, within the BiCE programme is to produce robust biocatalysts and to have many examples of each enzyme type available in our ‘toolbox’ to enable the rapid development of new biocatalytic steps and pathways. The programme brings together a group of researchers from UCL with a long history of collaborative research including Prof Helen Hailes (Chemistry, UCL) together with Prof Paul Dalby, Prof Gary Lye, Dr Frank Baganz, Dr Martina Micheletti, Dr. Darren Nesbeth and Dr Nicolas Szita. It is supported by a group of 13 leading national and international companies who comprise the BiCE Industrial Steering Group. In addition, with Prof Helen Hailes in the Department of Chemistry, John is developing the de novo construction of biosynthetic pathways for chiral molecules such as plant alkaloids and recently has discovered antimicrobial activities in novel compounds from this alkaloid project. John is using metagenomics which is a non culturing method of accessing novel enzymes and pathways from the microbiomes of various environments such as the human tongue with Prof Brian Henderson. John collaborates with Prof Christine Orengo (SMB, UCL) who provides the bioinformatics support for the metagenomics projects.The hunt for novel enzymes extends to collaborations in Astrobiology with Dr Ian Crawford and Prof Peter Muller, and the UCL Origin of Life Reactor a Leverhulme Trust funded project with Dr Nick Lane and Dr Julian Evans. The future inputs for the chemical and pharmaceutical industry need to move from non-renewable petrochemicals to sustainable materials such as plant biomass. The whole bioprocess from the physical, enzymic and chemical treatment to separate the biopolymers such as cellulose, hemicelluolose, pectin and protein through to the depolymerisation of the key polymers and the use of synthetic biology to create engineered pathways to make high value products is being tackled. John is the lead in Synbion, one of the UK’s Networks in Synthetic Biology

Basic Life Sciences Bioprocessing Nanotechnology Origins Personalised Medicine Synthetic Biology

近期论文

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A cell engineering strategy to enhance supercoiled plasmid DNA production for gene therapy Journal article; 2016; Biotechnology and Bioengineering Links: IRIS | UCL Discovery | DOI Authors: Hassan S,Keshavarz-Moore E,Ward J click to collapse Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis Journal article; 2016; ChemistrySelect Links: IRIS | DOI Authors: Jeffries JWE,Dawson N,Orengo C,Moody TS,Quinn DJ,Hailes HC,Ward JM click to collapse Micromolar colorimetric detection of 2-hydroxy ketones with the water-soluble tetrazolium WST-1 Journal article; 2016; ANALYTICAL BIOCHEMISTRY Links: IRIS | Author's | DOI Authors: Bommer M,Ward JM click to collapse Novel Computational Protocols for Functionally Classifying and Characterising Serine Beta-Lactamases Journal article; 2016; PLoS Computational Biology Links: IRIS | UCL Discovery | DOI Authors: Lee D,Das S,Dawson NL,Dobrijevic D,Ward J,Orengo C click to collapse Optimising cell design steps for efficient removal of E. coli host cell DNA impurities from Fab' fragment process streams. Conference presentation; 2016; Links: IRIS | UCL Discovery | Publisher Authors: Schofield D,Sirka E,Ward JM,Keshavarz-moore E,Nesbeth DN click to collapse Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose Journal article; 2016; GREEN CHEMISTRY Links: IRIS | UCL Discovery | Author's | DOI Authors: Subrizi F,Cardenas-Fernandez M,Lye GJ,Ward JM,Dalby PA,Sheppard TD,Hailes HC click to collapse Dopamine-first' mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile Journal article; 2015; FEBS JOURNAL Links: IRIS | UCL Discovery | Author's | DOI Authors: Lichman BR,Gershater MC,Lamming ED,Pesnot T,Sula A,Keep NH,Hailes HC,Ward JM click to collapse A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase Journal article; 2015; CHEMICAL COMMUNICATIONS Links: IRIS | UCL Discovery | Author's | DOI Authors: Baud D,Ladkau N,Moody TS,Ward JM,Hailes HC click to collapse CATH FunFHMMer web server: protein functional annotations using functional family assignments Journal article; 2015; NUCLEIC ACIDS RESEARCH Links: IRIS | UCL Discovery | Author's | DOI Authors: Das S,Sillitoe I,Lee D,Lees JG,Dawson NL,Ward J,Orengo CA click to collapse Isolation of Radiation-Resistant Bacteria from Mars Analog Antarctic Dry Valleys by Preselection, and the Correlation between Radiation and Desiccation Resistance Journal article; 2015; ASTROBIOLOGY Links: IRIS | Author's | DOI Authors: Musilova M,Wright G,Ward JM,Dartnell LR click to collapse Modelling and optimisation of the one-pot, multi-enzymatic synthesis of chiral amino-alcohols based on microscale kinetic parameter determination Journal article; 2015; CHEMICAL ENGINEERING SCIENCE Links: IRIS | UCL Discovery | Author's | DOI Authors: Rios-Solis L,Morris P,Grant C,Odeleye AOO,Hailes HC,Ward JM,Dalby PA,Baganz F,Lye GJ click to collapse Multi-step biocatalytic strategies for chiral amino alcohol synthesis Journal article; 2015; ENZYME AND MICROBIAL TECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Villegas-Torres MF,Martinez-Torres RJ,Cazares-Koerner A,Hailes H,Baganz F,Ward J click to collapse omega-Transaminases for the amination of functionalised cyclic ketones Journal article; 2015; ORGANIC & BIOMOLECULAR CHEMISTRY Links: IRIS | UCL Discovery | Author's | DOI Authors: Richter N,Simon RC,Lechner H,Kroutil W,Ward JM,Hailes HC click to collapse One-pot triangular chemoenzymatic cascades for the syntheses of chiral alkaloids from dopamine Journal article; 2015; GREEN CHEMISTRY Links: IRIS | Author's | DOI Authors: Lichman BR,Lamming ED,Pesnot T,Smith JM,Hailes HC,Ward JM click to collapse Single active-site mutants are sufficient to enhance serine:pyruvate -transaminase activity in an -transaminase Journal article; 2015; FEBS JOURNAL Links: IRIS | Author's | DOI Authors: Deszcz D,Affaticati P,Ladkau N,Gegel A,Ward JM,Hailes HC,Dalby PA click to collapse Tetrahydroisoquinolines affect the whole-cell phenotype of Mycobacterium tuberculosis by inhibiting the ATP-dependent MurE ligase Journal article; 2015; JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY Links: IRIS | UCL Discovery | Author's | DOI Authors: Guzman JD,Pesnot T,Barrera DA,Davies HM,McMahon E,Evangelopoulos D,Mortazavi PN,Munshi T,Maitra A,Lamming ED,Angell R,Gershater MC,Redmond JM,Needham D,Ward JM,Cuca LE,Hailes HC,Bhakta S click to collapse The protein fraction from wheat-based dried distiller's grain with solubles (DDGS): extraction and valorization Journal article; 2015; NEW BIOTECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Villegas-Torres MF,Ward JM,Lye GJ click to collapse Use of the M13 viral chassis, fluid dynamics and linear dichroism in pathogen detection Journal article; 2015; EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS Links: IRIS | Author's Authors: Pacheco-Gomez R,Carr-Smith J,Hicks MR,Sandhu S,Haydn LA,Daniella LP,Arkill KP,Rodger A,Ward J,Tucker J,Dafforn TR click to collapse A composition Patent; 2014; Links: IRIS Authors: Ward JM,Hales JE,Aeppli G,Daffron T click to collapse An origin-of-life reactor to simulate alkaline hydrothermal vents. Journal article; 2014; J Mol Evol Links: IRIS | UCL Discovery | Author's | DOI Authors: Herschy B,Whicher A,Camprubi E,Watson C,Dartnell L,Ward J,Evans JR,Lane N click to collapse Efficient 2-step biocatalytic strategies for the synthesis of all nor(pseudo) ephedrine isomers Journal article; 2014; GREEN CHEMISTRY Links: IRIS | UCL Discovery | Author's | DOI Authors: Sehl T,Hailes HC,Ward JM,Menyes U,Pohl M,Rother D click to collapse Evaluation of CV2025 omega-transaminase for the bioconversion of lignin breakdown products into value-added chemicals: synthesis of vanillylamine from vanillin Journal article; 2014; BIOCATALYSIS AND BIOTRANSFORMATION Links: IRIS | UCL Discovery | Author's | DOI Authors: Du CJ,Rios-Solis L,Ward JM,Dalby PA,Lye GJ click to collapse Extraction of the protein fraction of dry distillers grains with solubles, implementing biocatalytic and chemical methods Journal article; 2014; NEW BIOTECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Torres MV,Lye G,Ward J click to collapse Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes. Journal article; 2014; Sci Rep Links: IRIS | UCL Discovery | Author's | DOI Authors: Grant C,Deszcz D,Wei YC,Martínez-Torres RJ,Morris P,Folliard T,Sreenivasan R,Ward J,Dalby P,Woodley JM,Baganz F click to collapse Long-term stabilization of reflective foams in sea water Journal article; 2014; RSC Adv. Links: IRIS | UCL Discovery | DOI Authors: Aziz A,Hailes HC,Ward JM,Evans JRG click to collapse