Nesbeth, Darren - University College London - Department of Biochemical Engineering

个人简介

Management Committee, UCL CDT in Bioprocess Leadership (2015-) Judging Committee, iGEM (2011-) Affiliate Member, Institution of Chemical Engineers (2010-) Member, Royal Society of Biologists (2010-) Co-Chair, Synthetic Biology Task Group, European Federation of Biotechnology (2011-2012) Steering Committee, UCL SynBion Synthetic Biology Network (2010-2012) EPSRC Postdoctoral Research Fellow, UCL (2007-2010) MRC Postdoctoral Research Associate, Imperial College London (2005-2007) BBSRC Postdoctoral Research Fellow, King’s College London (2002-2004) Capital Markets Communications Consultant, Buchanan Communications (2000-2001)

研究领域

Darren N. Nesbeth's research is focused on the industrial application of synthetic biology for the re-design of living cells for improved upstream and downstream bioprocessing. His main area of expertise is in the use of molecular biology, intracellular trafficking and protein engineering to design cells with useful functions that are also robust to industrial environments. His research experience spans working with Escherichia coli, Pichia pastoris and Chinese hamster ovary (CHO) cells in the fields of biopharmaceutical manufacturing, cell and gene therapy and whole cell biocatalysis. Darren coordinates the EUR1.86M ERA-IB 'IPCRES - Integrated Process and Cell Refactoring System' consortium of 8 partners across Europe including Ingenza and SilicoLife. In addition to applied research Darren also investigates the fundamental properties of synthetic gene networks and the biologically realisible design of cells that autonomously optimise their ability to perform a pre-programmed function. His research has also explored the feasibility of establishing synthetic biology standards in bioprocess monitoring and genome design.

近期论文

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Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrickTM design, serum-free virus production and microcarrier-based cultivation of CV-1 cells. Journal article; 2017; Heliyon Links: IRIS Authors: Liu S,Ruban L,Zhou Y,Wang Y,Nesbeth DN click to collapse A high performance bench scale process for isolation from inclusion bodies, refolding and dimerisation of a thiol-engineered recombinant therapeutic protein. Journal article; 2016; Biotechnology and Bioprocess Engineering Links: IRIS Authors: Schofield DM,Nesbeth DN click to collapse A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction Journal article; 2016; SpringerPlus Links: IRIS | UCL Discovery Authors: Templar A,Marsh D,Nesbeth DN click to collapse Bacterial Cells as Engineered Chassis. Chapter; 2016; Links: IRIS | UCL Discovery | Publisher Authors: Borg Y,Templar A,Schofield D,Nesbeth DN click to collapse Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrickTM design, serum-free virus production and microcarrier-based cultivation of CV-1 cells. Working discussion paper; 2016; Links: IRIS | DOI | Publisher Authors: Liu S,Ruban L,Wang Y,Zhou Y,Nesbeth DN click to collapse Eukaryotae Synthetica: Synthetic Biology in Yeast, Microalgae and Mammalian Cells. Chapter; 2016; Links: IRIS | UCL Discovery | Author's Authors: Schofield D,Templar A,Borg Y,Daer R,Haynes K,Nesbeth DN click to collapse Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring Journal article; 2016; JOURNAL OF MICROBIOLOGICAL METHODS Links: IRIS | UCL Discovery | Author's | DOI Authors: Templar A,Woodhouse S,Keshavarz-Moore E,Nesbeth DN click to collapse Measuring E. coli and bacteriophage DNA in cell sonicates to evaluate the CAL1 reaction as a synthetic biology standard for qPCR. Journal article; 2016; Biomolecular Detection and Quantification Links: IRIS Authors: Templar A,Schofield DM,Nesbeth DN click to collapse Open source approaches to establishing Roseobacter clade bacteria as synthetic biology chassis for biogeoengineering. Journal article; 2016; PeerJ Links: IRIS | DOI Authors: Borg Y,Grigonyte A,Boeing P,Wolfenden B,Smith P,Beaufoy W,Rose S,Ratisai T,Zaikin A,Nesbeth DN click to collapse Open source approaches to establishing Roseobacter clade bacteria as synthetic biology chassis for biogeoengineering. Fig 2 raw data Dataset; 2016; Links: IRIS | DOI Authors: Borg Y,Grigonyte A,Boeing P,Wolfenden B,Smith P,Beaufoy W,Rose S,Ratisai T,Zaikin A,Nesbeth D click to collapse Open source approaches to establishing Roseobacter clade bacteria as synthetic biology chassis for biogeoengineering. Fig 3 raw data Dataset; 2016; Links: IRIS | DOI Authors: Borg Y,Grigonyte A,Boeing P,Wolfenden B,Smith P,Beaufoy W,Rose S,Ratisai T,Zaikin A,Nesbeth D click to collapse Open source approaches to establishing Roseobacter clade bacteria as synthetic biology chassis for biogeoengineering. Fig 4 raw data Dataset; 2016; Links: IRIS | DOI Authors: Borg Y,Grigonyte A,Boeing P,Wolfenden B,Smith P,Beaufoy W,Rose S,Ratisai T,Zaikin A,Nesbeth D click to collapse Optimising cell design steps for efficient removal of E. coli host cell DNA impurities from Fab' fragment process streams. Conference presentation; 2016; Links: IRIS | UCL Discovery | Publisher Authors: Schofield D,Sirka E,Ward JM,Keshavarz-moore E,Nesbeth DN click to collapse Promoter engineering to optimise recombinant periplasmic Fab' fragment production in Escherichia coli. Journal article; 2016; Biotechnology Progress Links: IRIS | UCL Discovery | DOI | Publisher Authors: Schofield D,Templar A,Newton J,Nesbeth DN click to collapse Synthetic biology approaches to bioprocess intensification in bacterial and mammalian production platforms Thesis/Dissertation; 2016; Links: IRIS Authors: Schofield D Synthetic Biology Handbook Book; 2016; Links: IRIS | Publisher Synthetic biology routes to bio-artificial intelligence Journal article; 2016; Essays in Biochemistry Links: IRIS Authors: Nesbeth DN,Zaikin A,Saka Y,Carmen Romano M,Giuraniuc CV,Kanakov O,Laptyeva T click to collapse Design, Mathematical Modelling, Construction and Testing of Synthetic Gene Network Oscillators to Establish Roseobacter Clade Bacteria and the Protozoan Trypanosoma brucei as Synthetic Biology Chassis. Thesis/Dissertation; 2015; Links: IRIS | UCL Discovery Authors: Borg Y Hyaluronan treatment of biotinylated packaging cells increases titre of progeny retrovirus affinity-captured onto paramagnetic particles. Journal article; 2015; The Winnower Links: IRIS | UCL Discovery | DOI | Publisher Authors: Nesbeth DN IMAC capture of recombinant protein from unclarified mammalian cell feed streams Journal article; 2015; Biotechnology and Bioengineering Links: IRIS | UCL Discovery | DOI Authors: Kinna A,Tolner B,Rota EM,Titechener-Hooker N,Nesbeth DN,Chester K click to collapse A serum-free adapted CV-1 cell line as a potential host cell line for an oncolytic vaccinia virus production Conference ; 2014; Links: IRIS | UCL Discovery Authors: Liu S,Wang Y,Nesbeth D,Ruban L,Zhou Y click to collapse Complex and unexpected dynamics in simple genetic regulatory networks. Journal article; 2014; International Journal of Modern Physics B Links: IRIS | UCL Discovery | Publisher Authors: Borg YB,Ekkehard U,Afnan A,Alsaedi A,Nesbeth D,Zaikin A click to collapse DIYbio:Academe Collaboration to Expand BioBrickTM Design Space into Marine Bacterial Chassis Conference ; 2014; Links: IRIS Authors: Borg Y,Grigonyte A,Lu Y,Boeing P,Wolfenden B,Ratisai T,Rose S,Gomez-Pereira P,Zaikin A,Nesbeth D click to collapse DIYbio:Academe collaboration to expand BioBrickTM design space into marine bacterial chassis. Conference presentation; Links: IRIS Authors: Nesbeth DN EDEM1 targets misfolded HLA-B27 dimers for endoplasmic reticulum associated degradation Journal article; 2014; Links: IRIS | UCL Discovery Authors: Guiliano DB,Fussell H,Lenart I,Tsao E,Nesbeth DN,Fletcher AJ,Campbell EC,Yousaf N,Williams S,Santos S,Cameron A,Towers GJ,Kellam P,Hebert DN,Gould K,Powis SJ,Antoniou AN click to collapse